Three-Dimensional Magnetically-Oriented Microcrystal Array: A Large Sample for Neutron Diffraction Analysis

Kimura, Tsunehisa; Kimura, Fumiko; Matsumoto, Kenji; Metoki, Naoto

doi:10.5772/35296

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2016

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“…Biaxial crystal alignments are achieved by the following mechanism (Kimura et al, 2012). The magnetic anisotropy of biaxial crystals, including the orthorhombic, monoclinic and triclinic systems, is described by their magnetic susceptibility tensor v, which is characterized by its principal values 1 , 2 and 3 (we define 1 > 2 > 3 ) and corresponding principal axes.…”

Section: Introductionmentioning

confidence: 99%

Neutron and X-ray single-crystal diffraction from protein microcrystalsviamagnetically oriented microcrystal arrays in gels

Tsukui

Kimura

Kusaka

et al. 2016

Acta Cryst Sect D Struct Biol

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Protein microcrystals magnetically aligned in D2O hydrogels were subjected to neutron diffraction measurements, and reflections were observed for the first time to a resolution of 3.4 Å from lysozyme microcrystals (∼10 × 10 × 50 µm). This result demonstrated the possibility that magnetically oriented microcrystals consolidated in D2O gels may provide a promising means to obtain single-crystal neutron diffraction from proteins that do not crystallize at the sizes required for neutron diffraction structure determination. In addition, lysozyme microcrystals aligned in H2O hydrogels allowed structure determination at a resolution of 1.76 Å at room temperature by X-ray diffraction. The use of gels has advantages since the microcrystals are measured under hydrated conditions.

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Section: Introductionmentioning

confidence: 99%