Three-Dimensional Measurement of Ice Crystals in Frozen Materials by Near-Infrared Imaging Spectroscopy

Do, Gabsoo; Araki, Toshimitsu; Bae, Yeonghwan; Ishikura, Ko; Sagara, Yasuyuki

doi:10.1080/07373937.2015.1029073

Cited by 8 publications

(7 citation statements)

References 25 publications

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“…Transmission electron microscopy can be used to image the growth of crystals and the behavior of particles repelled by the latter, but such experiments cannot yet be run routinely and the interaction of the electron beam with the sample is always questionable . Near-infrared imaging spectroscopy was used to investigate the morphology of ice crystals in biological materials, but freezing was not performed in situ and the spatial resolution is still not sufficient to image small crystals …”

Section: Introductionsupporting

confidence: 54%

Section: Introductionmentioning

confidence: 99%

“…26 Near-infrared imaging spectroscopy was used to investigate the morphology of ice crystals in biological materials, but freezing was not performed in situ and the spatial resolution is still not sufficient to image small crystals. 27 Here we show how confocal laser scanning microscopy and image analysis can be used to investigate the growth of ice crystals as small as tens of micrometers in situ in ZrAc solutions, stained with sulforhodamine B, in a confined thin slab. We will show that we can follow and reconstruct the ice crystals topography and quantitatively estimate the kinetics of the advancing ice front while freezing using a table-top instrument.…”

Section: ■ Introductionmentioning

confidence: 99%

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Time-Lapse, in Situ Imaging of Ice Crystal Growth Using Confocal Microscopy

et al. 2016

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Ice crystals nucleate and grow when a water solution is cooled below its freezing point. The growth velocities and morphologies of the ice crystals depend on many parameters, such as the temperature of ice growth, the melting temperature, and the interactions of solutes with the growing crystals. Three types of morphologies may appear: dendritic, cellular (or fingerlike), or the faceted equilibrium form. Understanding and controlling which type of morphology is formed is essential in several domains, from biology to geophysics and materials science. Obtaining, in situ, three dimensional observations without introducing artifacts due to the experimental technique is nevertheless challenging. Here we show how we can use laser scanning confocal microscopy to follow in real-time the growth of smoothed and faceted ice crystals in zirconium acetate solutions. Both qualitative and quantitative observations can be made. In particular, we can precisely measure the lateral growth velocity of the crystals, a measure otherwise difficult to obtain. Such observations should help us understand the influence of the parameters that control the growth of ice crystals in various systems.

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Section: Introductionsupporting

confidence: 54%

Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

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Time-Lapse, in Situ Imaging of Ice Crystal Growth Using Confocal Microscopy

et al. 2016

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“…4). Ice crystals were also identified by NIR imaging spectroscopy [8]. The NIR images of ice crystals in the frozen sample appeared darker than the other components at 1500 nm due to weak reflected light at this peak wavelength.…”

Section: Recognition Of Bubbles Ice Crystals and Milk Solidsmentioning

confidence: 99%

“…The MSIPS [4][5][6] has been applied to measure the three-dimensional (3D) morphology of agricultural products with complex shapes and to identify ice crystals in frozen materials. The CMSIS [7,8] has a function to eliminate pre-treatment processes such as fluorescence staining, freeze-substitution, and freeze dehydration by adapting a near-infrared (NIR) spectroscopic imaging technique. The physical changes of samples dur- ing pre-treatment processes could thus be eliminated.…”

Section: Introductionmentioning

confidence: 99%

Microscale to Macroscale Measurement of Bubbles in Frozen Materials with Cryogenic Microtome Spectral Imaging System

Sase

Bae

et al. 2017

Japan J. Food Eng.

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The internal structures of frozen materials and the formation of bubbles significantly affect the physical properties of such materials as ice cream. Various measurement techniques including optical and scanning electron microscopies, and X-ray microcomputed tomography have been employed to obser ve bubbles and the internal structures of frozen samples. However, these measurement techniques revealed only a limited measurement range and low resolution. The objectives of this work were to develop a cr yogenic microtome spectral imaging system (CMtSIS) to measure microscale to macroscale with respect to bubbles and internal structures formed in some frozen materials. The CMtSIS, which consisted of a microtome, automatic high-precision XY stage and a near-infrared (NIR) spectral imaging system, enable the capture of consecutive cross-sectional images of a frozen sample exposed by multi-slicing with a minimum thickness of 0.001 mm and a maximum area of 60× 60 mm. Bubbles in an ice cream sample were identified as defocused spots in two-dimensional (2D) CMtSIS images due to the difference in the focal distance created by the vacant spaces. NIR images of ice crystals in the frozen sample appeared darker than the other components at 1500 nm due to weak reflected light at this peak wavelength. Milk solids were also observed by the flux differences in light reflected from the different interfaces in the ice cream.

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Investigating the Freezing of Colloids: Experimental Techniques to Probe Solidification Patterns, Crystal Growth, and Particle Movement

Deville

2017

Engineering Materials and Processes

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Three-Dimensional Measurement of Ice Crystals in Frozen Materials by Near-Infrared Imaging Spectroscopy

Cited by 8 publications

References 25 publications

Time-Lapse, in Situ Imaging of Ice Crystal Growth Using Confocal Microscopy

Time-Lapse, in Situ Imaging of Ice Crystal Growth Using Confocal Microscopy

Microscale to Macroscale Measurement of Bubbles in Frozen Materials with Cryogenic Microtome Spectral Imaging System

Investigating the Freezing of Colloids: Experimental Techniques to Probe Solidification Patterns, Crystal Growth, and Particle Movement

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