Three-dimensional microstructured tissue scaffolds fabricated by two-photon laser scanning photolithography

Hsieh, Tseng Ming; Ng, Chien Wei Benjamin; Narayanan, Karthikeyan; Wan, Andrew C.A.; Ying, Jackie Y.

doi:10.1016/j.biomaterials.2010.06.029

Cited by 70 publications

(49 citation statements)

References 17 publications

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“…c o m / l o c a t e / m s e c 3D structures over a large area. Therefore we are able to overcome the limitations of polymer processing methods mentioned by Hsieh [13] and Demirbag [14], namely the lack of control over parameters such as pore geometry and permeability. The herein presented method enables to control 3D structuring and shaping of the porous material without destroying the initial microstructure and connections between pores.…”

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 98%

Thermoforming techniques for manufacturing porous scaffolds for application in 3D cell cultivation

Borowiec

Hampl

Gebinoga

et al. 2015

Materials Science and Engineering: C

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Section: Contents Lists Available At Sciencedirectmentioning

confidence: 98%

Thermoforming techniques for manufacturing porous scaffolds for application in 3D cell cultivation

Borowiec

Hampl

Gebinoga

et al. 2015

Materials Science and Engineering: C

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“…As mentioned before, conventional lithographic techniques, relying on single-photon absorption, provide limited spatial resolution on the order of a few tens of microns at best. In contrast, multiphoton lithography (or multiphoton processing) has recently proven to be a very promising technological platform for overcoming this limitation [11,80,[107][108][109][110][111].…”

Section: Multiphoton Lithographymentioning

confidence: 99%

Additive manufacturing of photosensitive hydrogels for tissue engineering applications

et al. 2014

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Hydrogels are extensively explored as scaffolding materials for 2D/3D cell culture and tissue engineering. Owing to the substantial complexity of tissues, it is increasingly important to develop 3D biomimetic hydrogels with user-defined architectures and controllable biological functions. To this end, one promising approach is to utilize photolithography-based additive manufacturing technologies (AMTs) in combination with photosensitive hydrogels. We here review recent advances in photolithography-based additive manufacturing of 3D hydrogels for tissue engineering applications. Given the importance of materials selection, we firstly give an overview of water-soluble photoinitiators for single-and two-photon polymerization, photopolymerizable hydrogel precursors and light-triggered chemistries for hydrogel formation.Through the text we discuss the design considerations of hydrogel precursors and synthetic approaches to polymerizable hydrogel precursors of synthetic and natural origins. Next, we shift to how photopolymerizable hydrogels could integrate with photolithography-based AMTs for creating well-defined hydrogel structures. We illustrate the working-principles of both single-and two-photon lithography and case studies of their applications in tissue engineering. In particular, two-photon lithography is highlighted as a powerful tool for 3D functionalization/construction of hydrogel constructs with μm-scale resolution. Within the text we also explain the chemical reactions involved in two-photon-induced biofunctionalization and polymerization. In the end, we summarize the limitations of available hydrogel systems and photolithography-based AMTs as well as a future outlook on potential optimizations.

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“…The current capability of TPP allows the generation of 3D structures with a resolution down to 100 nm [10]. For tissue engineering applications, TPP has been used to manufacture 3D scaffolds for hepatocyte culture by triggering freeradical polymerization processes within polymerizable precursors and to fabricate micro-patterns of biomolecules for guiding cell migration within 3D PEG-based hydrogels [11,12]. The hydrogel network produced with well-defined chemical and physical properties has been further functionalized and modified for elucidating more complex relationships in cell behavior and tissue development and also for introducing pathways to engineer complex tissues [13].…”

Section: Two-photon Polymerizationmentioning

confidence: 99%

Selective Laser Sintering and Its Biomedical Applications

Duan

Wang

2013

Laser Technology in Biomimetics

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Selective laser sintering (SLS), a mature and versatile rapid prototyping (RP) technology, uses a laser beam to selectively sinter powdered materials to form three-dimensional objects, porous or non-porous, according to the computer-aided design which can be based on data obtained from advanced medical imaging technologies such as magnetic resonance imaging (MRI) and computer tomography (CT). In this chapter, major RP technologies suitable for biomedical applications are briefly introduced first. A review is made on SLS, including its working principle, modification of commercial SLS machines for fabricating biomedical products, biomedical SLS materials, and optimization of SLS parameters. Finally, a detailed presentation is given on the biomedical application of SLS, focusing on the fabrication of tissue engineering scaffolds and drug or biomolecule delivery vehicles. It is shown that SLS has great potential for many biomimetic and biomedical applications.

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Three-dimensional microstructured tissue scaffolds fabricated by two-photon laser scanning photolithography

Cited by 70 publications

References 17 publications

Thermoforming techniques for manufacturing porous scaffolds for application in 3D cell cultivation

Thermoforming techniques for manufacturing porous scaffolds for application in 3D cell cultivation

Additive manufacturing of photosensitive hydrogels for tissue engineering applications

Selective Laser Sintering and Its Biomedical Applications

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