Three‐dimensional motility cycle in leukocytes

Murray, John D.; Vawter‐Hugart, Holly; Voss, Edward; Soll, David R.

doi:10.1002/cm.970220308

Cited by 60 publications

(46 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Dictyostelium cells are similar to leukocytes, in that they are fast-moving cells with an irregular shape (Murray et al, 1992;Wessels et al, 1994). Talin (Kreitmeier et al, 1995) and alphaactinin (Brier et al, 1983) have been identified as adhesionrelated proteins and their subcellular localization is similar to that of actin.…”

mentioning

confidence: 91%

Dynamics of novel feet of Dictyostelium cells during migration

Uchida

Yumura

2004

Journal of Cell Science

View full text Add to dashboard Cite

We observed the dynamics of actin foci in live Dictyostelium cells expressing GFP-actin. Actin foci were dynamic structures, but they were fixed on the substratum during cell migration. Interference reflection microscopy revealed that the ventral cell membrane was closer to the substratum at sites of actin foci. Furthermore, some actin foci were incorporated into the retraction fibers, ripped off from the cells and eventually shed on the substratum after the cells moved away. The velocity of the cells was inversely proportional to the number of actin foci. Measurement of traction force using a silicone substratum demonstrated that the traction force was transmitted to the substratum through actin foci. Taken together, several lines of evidence strongly suggest that actin foci function as the active `feet' of Dictyostelium cells. We also found evidence suggesting that changing step is regulated in a coordinated manner during cell migration. Possible mechanisms by which these cells migrate across substrata are discussed in this context.

show abstract

mentioning

confidence: 91%

Dynamics of novel feet of Dictyostelium cells during migration

Uchida

Yumura

2004

Journal of Cell Science

View full text Add to dashboard Cite

show abstract

“…An example of switching migration strategy has been reported in the case of tumor cells, which can convert from a mesenchymal to an amoeboid motility mode following metalloproteinase inhibition (9,10). In addition, studies in the amoeba Dictyostelium (11) and in neutrophils (12) have suggested the possibility of motility kinetics with discontinuous adhesion characteristics, but the causes and molecular details remain unknown. Given the number of T cell functions that rely on migration to specific tissues and interaction with other cells (8,13), we sought to examine the general principles of motility in lymphocytes, particularly in relation to substrate adhesion and motility modes.…”

mentioning

confidence: 99%

Myosin-IIA and ICAM-1 Regulate the Interchange between Two Distinct Modes of T Cell Migration

Jacobelli

Bennett²,

Pandurangi³

et al. 2009

The Journal of Immunology

112

137

View full text Add to dashboard Cite

How T cells achieve rapid chemotactic motility under certain circumstances and efficient cell surface surveillance in others is not fully understood. We show that T lymphocytes are motile in two distinct modes: a fast “amoeboid-like” mode, which uses sequential discontinuous contacts to the substrate; and a slower mode using a single continuously translating adhesion, similar to mesenchymal motility. Myosin-IIA is necessary for fast amoeboid motility, and our data suggests that this occurs via cyclical rear-mediated compressions that eliminate existing adhesions while licensing subsequent ones at the front of the cell. Regulation of Myosin-IIA function in T cells is thus a key mechanism to regulate surface contact area and crawling velocity within different environments. This can provide T lymphocytes with motile and adhesive properties that are uniquely suited toward alternative requirements for immune surveillance and response.

show abstract

“…The 4D description of the basic motile behavior of D. discoideum amoebae presented here provides a contextual framework for developing models of motility, interpreting behavioral defects of mutants, deducing the roles cytoskeletal and regulatory molecules play in motility, and determining how chemotactic gradients manipulate basic motile behavior. Studies are now in progress to test which of the characteristics of D. discoideum motility hold true for other cell types, most notably human polymorphonuclear leukocytes (48,66), and how they are manipulated by spatial and temporal gradients of the chemoattractant cyclic AMP (63).…”

Section: Discussionmentioning

confidence: 99%

How a Cell Crawls and the Role of Cortical Myosin II

et al. 2009

Self Cite

View full text Add to dashboard Cite

The movements of Dictyostelium discoideum amoebae translocating on a glass surface in the absence of chemoattractant have been reconstructed at 5-second intervals and motion analyzed by employing 3D-DIAS software. A morphometric analysis of pseudopods, the main cell body, and the uropod provides a comprehensive description of the basic motile behavior of a cell in four dimensions (4D), resulting in a list of 18 characteristics. A similar analysis of the myosin II phosphorylation mutant 3XASP reveals a role for the cortical localization of myosin II in the suppression of lateral pseudopods, formation of the uropod, cytoplasmic distribution of cytoplasm in the main cell body, and efficient motility. The results of the morphometric analysis suggest that pseudopods, the main cell body, and the uropod represent three motility compartments that are coordinated for efficient translocation. It provides a contextual framework for interpreting the effects of mutations, inhibitors, and chemoattractants on the basic motile behavior of D. discoideum. The generality of the characteristics of the basic motile behavior of D. discoideum must now be tested by similar 4D analyses of the motility of amoeboid cells of higher eukaryotic cells, in particular human polymorphonuclear leukocytes.Amoeboid behavior is a characteristic of cells spanning the entire animal kingdom (5,6,15,56). It is basic to the life history of soil amoebae, embryogenesis, white blood cell function, tissue regeneration, neural development, and cancer metastasis (52). Therefore, the molecular biology of this process has been the focus of intensive investigation (2,15,40,50,53,57). In marked contrast, the basic motile behavior of amoeboid cells, which provides the contextual framework for interpreting the results of genetic and biochemical studies, has received far less attention. We still lack an accurate and quantitative fourdimensional (4D) description of how an amoeboid cell translocates along a substratum in the absence of a chemotactic signal. The lack of such a description has led to perceptions of single cell migration that are 2D, sometimes oversimplified, and sometimes actually inaccurate. These perceptions, unfortunately, have served as the basis for modeling cell locomotion, interpreting the behavioral defects of cytoskeletal and regulatory mutants, and assessing the effects of inhibitors and stimuli, most notably chemoattractants.To obtain a 4D description of cell behavior, we have employed 3D-DIAS software (25,61,62,64,78) to reconstruct and motion analyze the basic motile behavior of Dictyostelium discoideum amoebae translocating along a substratum in the absence of chemoattractant (63,71,76,77,79,80). By quantitating in space and time the extension and retraction of pseudopods, changes in shape, anterior progress of the main cell body and uropod, and contact of the ventral surface of the cell with the substratum, we have developed a complex morphometric description of cell migration that reveals a number of characteristics. In addition, by simil...

show abstract

Three‐dimensional motility cycle in leukocytes

Cited by 60 publications

References 27 publications

Dynamics of novel feet of Dictyostelium cells during migration

Dynamics of novel feet of Dictyostelium cells during migration

Myosin-IIA and ICAM-1 Regulate the Interchange between Two Distinct Modes of T Cell Migration

How a Cell Crawls and the Role of Cortical Myosin II

Contact Info

Product

Resources

About