Three-dimensional neuronal cell culture: in pursuit of novel treatments for neurodegenerative disease

Carter, Sarah‐Sophia D.; Liu, Xiao; Yue, Zhilian; Wallace, Gordon G.

doi:10.1557/mrc.2017.96

Cited by 5 publications

(9 citation statements)

References 86 publications

(118 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…7,9,50,52−54 Other interesting applications are focusing on neurodegenerative diseases. 17 In all these cases, the fabrication technique should ideally be highly repeatable, fast, and simple while providing high versatility and capability to rapidly adapt parameters to the specific cellular model. The size and aspect ratio of pillars can be easily finely tuned by properly changing the writing parameters and conditions employed during the PDMS mold mask-less fabrication, namely, the laser source pulse power, number of pulses, repetition rate, and pressure of the vacuum chamber.…”

Section: Resultsmentioning

confidence: 99%

“…4,15,16 Electrically inert polymer substrates (among many others, PDMS, SU-8, polycarbonate, PLA) have been largely employed as well, mainly for tissue engineering and regenerative medicine applications. 17,18 A variety of synthetic and bioderived polymers have been also developed ad hoc as biocompatible scaffolds for 3D cell cultures. Their distinct advantages over inorganic materials comprise easier and faster processing, increased design flexibility and versatility, softness, and outstanding biocompatibility.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

High-Aspect-Ratio Semiconducting Polymer Pillars for 3D Cell Cultures

Tullii

Giona

Lodola

et al. 2019

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Hybrid interfaces between living cells and nano/microstructured scaffolds have huge application potential in biotechnology, spanning from regenerative medicine and stem cell therapies to localized drug delivery and from biosensing and tissue engineering to neural computing. However, 3D architectures based on semiconducting polymers, endowed with responsivity to visible light, have never been considered. Here, we apply for the first time a push-coating technique to realize high aspect ratio polymeric pillars, based on polythiophene, showing optimal biocompatibility and allowing for the realization of soft, 3D cell cultures of both primary neurons and cell line models. HEK-293 cells cultured on top of polymer pillars display a remarkable change in the cell morphology and a sizable enhancement of the membrane capacitance due to the cell membrane thinning in correspondence to the pillars’ top surface, without negatively affecting cell proliferation. Electrophysiology properties and synapse number of primary neurons are also very well preserved. In perspective, high aspect ratio semiconducting polymer pillars may find interesting applications as soft, photoactive elements for cell activity sensing and modulation.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

High-Aspect-Ratio Semiconducting Polymer Pillars for 3D Cell Cultures

Tullii

Giona

Lodola

et al. 2019

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

“…[136] In recent years, the integration of 3D bioprinting with temporal dynamics, known as 4D bioprinting, has opened new possibilities to create dynamic bioengineered structures. [153] Indeed, structures which can change form or function within a certain time frame, in response to external stimuli such as temperature, water, pressure, or light, present important advantages in adapting to the dynamic needs of the tissue constructs. [154,155] A collection of dynamic biomaterials, such as supramolecular biomaterials, [150] thermo-responsive biomaterials, self-folding swelling gels, and shape memory materials (SMMs) [156][157][158] has emerged in the past few years.…”

Section: Synthetic Polymersmentioning

confidence: 99%

Emerging Three‐Dimensional Integrated Systems for Biomimetic Neural In Vitro Cultures

Seiti

Ginestra

Ceretti

et al. 2022

Adv Materials Inter

View full text Add to dashboard Cite

lial cells, and pericytes. [2] The intricate interconnections and spatial organization between neurons as well as between neuronal and non-neuronal cells enable the functional complexity and diversity of the brain, which ultimately generates motor and sensory function, as well as cognitive processes such as memory, learning, and emotions.Despite its critical functions, the brain has very limited ability to self-repair and regenerate upon neurological disease or injury. [3] The regeneration of damaged tissue in the brain after trauma is primarily impeded by glial scar formation and reactive astrocytes. [4] Conversely, neurodegenerative diseases are characterized by progressive dysfunction and loss of subpopulation of specialized cells such as dopaminergic neurons and oligodendrocytes. [5] Moreover, a number of changes occur in the brain extracellular matrix (bECM) in pathological situations, such as alterations in the expression of proteoglycans, protein aggregation, and amyloidosis during the progression of Alzheimer's disease (AD). [6] To investigate the mechanisms of regeneration and degeneration as well as those which regulate neural circuit formation, morphogenesis, and development, animal models have traditionally been the main research modality. However, these models have presented major challenges due to their genetic, biochemical, and metabolic differences to human physiology, along with the need for expensive and time-consuming protocols and associated ethical issues. In vitro models have been established as efficient alternatives, allowing recreating in vivolike micro-environments to study cellular differentiation, electrical activity, and their relationship to molecular signaling. Additionally, 3D in vitro models represent processes such as cell adhesion, migration, as well as of nutrient and metabolic waste transport in a more biomimetic context than conventional 2D culture. [7][8][9] Multidisciplinary approaches in the field of tissue engineering have emerged since the 1990s with the goal of fabricating biocompatible 3D bioengineered architectures composed of predefined compositions of cells, biomaterials, and biological factors. Ideally, these elements can be combined to generate biomimetic ECM formation and cellular phenotypes. [10][11][12] In parallel with technical advances in materials and fabrication techniques, the development of stem cell biology, and in particular the advent of 3D in vitro neural cultures have gained interest in recent years as promising biomimetic micro-environments which can regulate cell response to neural guidance cues. Several bioengineered technologies have been developed in combination with biomaterials to produce 3D models, such as scaffoldbased neural constructs, neurospheres, cerebral organoids, and brain-onchip devices. While the strategic selection of bioengineering approaches and associated biomaterials has opened a multitude of opportunities in building increasingly advanced neural tissues, technical limitations remain a challenge in modeling the complexity of ...

show abstract

“…Extrusion printing requires biomaterials to have sufficient rheological properties, such as a high enough viscosity, to maintain the strand shape prior to crosslinking. Extrusion printing typically has lower resolution than inkjet or laser systems [154,155].…”

Section: Strategies For Biofabricating Neural Modelsmentioning

confidence: 99%

“…Extrusion printing is commonly employed in bioprinting due to its economy, ease of use, and capability to print with high cell density with a wide range of materials [154,170]. Resolution is dependent upon nozzle diameter typically in the range of 50–500 µm [155].…”

Section: 3d Bioprintingmentioning

confidence: 99%

Layer-By-Layer: The Case for 3D Bioprinting Neurons to Create Patient-Specific Epilepsy Models

2019

View full text Add to dashboard Cite

The ability to create three-dimensional (3D) models of brain tissue from patient-derived cells, would open new possibilities in studying the neuropathology of disorders such as epilepsy and schizophrenia. While organoid culture has provided impressive examples of patient-specific models, the generation of organised 3D structures remains a challenge. 3D bioprinting is a rapidly developing technology where living cells, encapsulated in suitable bioink matrices, are printed to form 3D structures. 3D bioprinting may provide the capability to organise neuronal populations in 3D, through layer-by-layer deposition, and thereby recapitulate the complexity of neural tissue. However, printing neuron cells raises particular challenges since the biomaterial environment must be of appropriate softness to allow for the neurite extension, properties which are anathema to building self-supporting 3D structures. Here, we review the topic of 3D bioprinting of neurons, including critical discussions of hardware and bio-ink formulation requirements.

show abstract

Three-dimensional neuronal cell culture: in pursuit of novel treatments for neurodegenerative disease

Cited by 5 publications

References 86 publications

High-Aspect-Ratio Semiconducting Polymer Pillars for 3D Cell Cultures

High-Aspect-Ratio Semiconducting Polymer Pillars for 3D Cell Cultures

Emerging Three‐Dimensional Integrated Systems for Biomimetic Neural In Vitro Cultures

Layer-By-Layer: The Case for 3D Bioprinting Neurons to Create Patient-Specific Epilepsy Models

Contact Info

Product

Resources

About