Three-Dimensional Organization of Lymphatics and their Relationship to Blood Vessels in Rabbit Small Intestine. A Scanning Electron Microscopic Study of Corrosion Casts

Tumor Cell Transendothelial Passage in the Absorbing Lymphatic Vessel of Transgenic Adenocarcinoma Mouse Prostate

“…In the second half of the 1900s, research on the lymphatic vascular system conducted using injection methods of coloring agents (Prussian blue and so forth), resins (Neoprene, metacrilate, Mercox, and so forth), and fluorescence micrography, the confocal microscopy, and lymphangioscintigraphy made significant contributions regarding the spatial distribution of lymphatic networks in different human organs and those of various animals. [1][2][3] Interesting results were also obtained using cinematographic documentation in vivo on the kinetics of valves and lymph flow. 4 In the last decade, the use of a series of molecules such as LYVE-1, Prox-1, podoplanin, D2-40, and vascular endothelial growth factor (VEGF) receptor-3 for identifying the lymphatic vessel [5][6][7][8] has made interesting contributions on the nature and function of the lymphatic vessel and the microenvironment surrounding it.…”

Structure of Lymphatics in Rat Cecum with Special Reference to Submucosal Collecting Lymphatics Endowed with Smooth Muscle Cells and Valves. I. A Scanning Electron Microscopic Study.

“…The arrowhead indicates the valve location. X 50 were prepared according to the method previously described by us (OHTANI and OHTSUKA, 1985;OHTANI and MURAKAMI, 1992a). The casting medium, a mixture of five volume Mercox (Oken Shoji, Tokyo) and one volume of monomeric methyl methacrylate, was puncture-injected intraparenchymally into the deep mucosa.…”

“…SEM has also disclosed the ultrastructure of both luminal and abluminal surfaces of the lymphatic endothelium (CASTENHOLZ, 1984;NAKAMURA, 1986;USHIKI, 1990). The three-dimensional distribution of the lymphatic system in some organs has been demonstrated by corrosion casting/SEM (CASTENHOLZ, 1984;OHTANI and OHTSUKA, 1985;OHTANI et al, 1986;OHTANI, 1987;OHTANI andMURAKAMI, 1990, 1992b). Recently methods have been developed to demonstrate the lymphatic system more precisely and equivocally.…”

Three-dimensional Organization of Lymphatics in the Dog Small Intestine: A Scanning Electron Microscopic Study on Corrosion Casts.

“…Using this method, the three-dimensional organization of Lymphatics in the small intestine of the rabbit and rat has been replicated by direct injection of Mercox resin into the intestinal parenchyma, to be observed by SEM (OHTANI and OHTSUKA, 1985;OHTANI, 1987). In the canine intestine, however, production of precise casts of lymphatics by resin injection into parenchyma was difficult.…”