Three‐dimensional organization of the elastic fibres in the rat lung

Gonçalves, Carlos Alberto; Figueiredo, Maria Helena; Bairos, Vasco

doi:10.1002/ar.1092430108

Cited by 18 publications

(15 citation statements)

References 12 publications

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“…Arising from the need to exploit and integrate their distinctly different mechanical properties, in animal tissues, elastic tissue fibers are almost constantly found in close topographical relationship to the collagenous fibers (Elden, 1968;Gosline, 1976;Gosline & French, 1979;Alter, 2004). In the compliant mammalian lung, elastic tissue occurs abundantly and diffusely (Gonçalves et al 1995;Tochima et al 2004). Carton et al (1964) observed that more elastic tissue was present in the lungs of two mammalian species than was theoretically expected.…”

Section: Discussionmentioning

confidence: 99%

“…the area containing the air and the blood capillaries, is literally 'suspended' between the axial (A) and peripheral (E) pillars by a dense network of collagen fibers. Gonçalves et al 1995;Maina, 2002;Tochima et al 2004). The corresponding structures of the avian lung have not been investigated in detail.…”

Section: Discussionmentioning

confidence: 99%

“…Once the latex set, it provided a scaffold that preserved the natural spatial arrangement of the CFs: in uncast preparations, during digestion, CFs folded, curled, and collapsed once the supporting lung tissue was removed. The procedure of combining casting and digestion was successfully used by Gonçalves et al (1995) to study the organization of the elastic fibers in the rat lung. Here, a 10-cm 3 syringe was used to inject latex rubber through the pulmonary trunk until moderate tension developed in the lung.…”

Section: Casting and Digestion Of The Lung For Collagen Fibersmentioning

confidence: 99%

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Implicit mechanistic role of the collagen, smooth muscle, and elastic tissue components in strengthening the air and blood capillaries of the avian lung

2010

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To identify the forces that may exist in the parabronchus of the avian lung and that which may explain the reported strengths of the terminal respiratory units, the air capillaries and the blood capillaries, the arrangement of the parabronchial collagen fibers (CF) of the lung of the domestic fowl, Gallus gallus variant domesticus was investigated by discriminatory staining, selective alkali digestion, and vascular casting followed by alkali digestion. On the luminal circumference, the atrial and the infundibular CF are directly connected to the smooth muscle fibers and the elastic tissue fibers. The CF in this part of the parabronchus form the internal column (the axial scaffold), whereas the CF in the interparabronchial septa and those associated with the walls of the interparabronchial blood vessels form the external, i.e. the peripheral, parabronchial CF scaffold. Thin CF penetrate the exchange tissue directly from the interparabronchial septa and indirectly by accompanying the intraparabronchial blood vessels. Forming a dense network that supports the air and blood capillaries, the CF weave through the exchange tissue. The exchange tissue, specifically the air and blood capillaries, is effectively suspended between CF pillars by an intricate system of thin CF, elastic and smooth muscle fibers. The CF course through the basement membranes of the walls of the blood and air capillaries. Based on the architecture of the smooth muscle fibers, the CF, the elastic muscle fibers, and structures like the interparabronchial septa and their associated blood vessels, it is envisaged that dynamic tensional, resistive, and compressive forces exist in the parabronchus, forming a tensegrity (tension integrity) system that gives the lung rigidity while strengthening the air and blood capillaries.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Casting and Digestion Of The Lung For Collagen Fibersmentioning

confidence: 99%

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Implicit mechanistic role of the collagen, smooth muscle, and elastic tissue components in strengthening the air and blood capillaries of the avian lung

2010

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“…In the acinus, elastin is present in the axial backbone of the alveolar ducts and alveolar entrances, as well as in the external sheath of the extra-alveolar microvessels [5][6][7][8]. This axial system is interconnected to the peripheral interstitial system that AFFILIATIONS *Rouen University Hospital, extends through the interlobular septa towards the pleura [8,9].…”

mentioning

confidence: 99%

Humanin vivofluorescence microimaging of the alveolar ducts and sacs during bronchoscopy

Thiberville¹,

Salaün²,

Lachkar³

et al. 2009

Eur Respir J

218

196

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The aim of the present study was to assess fibred confocal fluorescence microscopy (FCFM) as a tool for imaging the alveolar respiratory system in vivo during bronchoscopy.A 488-nm excitation wavelength FCFM device was used in 41 healthy subjects including 17 active smokers. After topical anaesthesia, the 1.4-mm miniprobe was introduced into the bronchoscope working channel and advanced distally to the alveoli. Morphometric and cellular analyses were performed on selected frames harbouring a minimal compression effect.In vivo acinar microimaging was obtained from each lung segment except for the apical and posterior segments of both upper lobes. Reproducible patterns, corresponding to the elastic framework of the axial and peripheral interstitial systems, were recorded from 192 separate acini. The mean¡SD thickness of the acinar elastic fibres was 10¡2.7 mm. Alveolar mouth diameters (mean¡SD 278¡53 mm) were normally distributed but appeared smaller in the right upper lobe and right medial basal segment. Lobular microvessels (median diameter 90 mm) were equally distributed throughout the lungs. Alveolar macrophages were not detectable in nonsmokers, whereas a specific tobacco-tar-induced fluorescence was observed in smoking subjects, providing fine details of the alveolar walls and macrophages. A strong correlation was found between the number of cigarettes smoked per day and the amount of large and mobile macrophages observed in vivo, as well as with the intensity of the macrophage alveolitis.Fibred confocal fluorescence microscopy enables accurate exploration of the peripheral lung in vivo in both smokers and nonsmokers.

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Lung elastic fibres from embryonic to adult birds

et al. 2008

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In mammals it is well known the role of the elastic fibres in the lung development and its fundamental importance in the process of distending and recoil of the organ as a whole [1]. The bird' lungs are compact and virtually non-expansible [2]. After morphological studies on elastic fibres distribution and quantification by image analysis [3] we carried out a study on biochemical methods to quantify elastin in the avian lung, using domestic fowl (Gallus gallus) as an experimental model. We made use of two analytical methods for the elastin determination in these animal lungs from the 14 th day of hatching until the 42 nd day after hatchling. As analytical methodologies we used high-performance liquid chromatography (HPLC) and a colorimetric method based on the use of a stain that has been only applied in histological techniques. The HPLC analysis was carried out in a reverse phase system with a binary gradient, detection by UV wavelength set at 254 nm [4]. By this way we were able to quantify desmosine and isodesmosine in the studied samples. The colorimetric method was used by applying a commercial kit ("Fastin™ Elastin Assay") which quantifies soluble tropoelastin and insoluble elastin made soluble (α-elastin) after treatment with hot oxalic acid. The results obtained by HPLC were statistically different if comparing the incubation period with the after-hatchling days (p<0,001). This fact is in agreement with the immaturity of elastin in the period of incubation, without the presence of cross-linkings. The determinations by the colorimetric method provided results without statistical difference between both analyzed periods (p=0,581). This confirms the presence of elastin in the incubation period since this method determines the soluble elastin (α-elastin) as well as the insoluble elastin made soluble after treatment with hot oxalic acid, in the after-hatchling period. Comparing the results obtained by both analytical methods, it is remarkable that only in the afterhatchling period there is a positive and significative relation between both methods.

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Three‐dimensional organization of the elastic fibres in the rat lung

Cited by 18 publications

References 12 publications

Implicit mechanistic role of the collagen, smooth muscle, and elastic tissue components in strengthening the air and blood capillaries of the avian lung

Implicit mechanistic role of the collagen, smooth muscle, and elastic tissue components in strengthening the air and blood capillaries of the avian lung

Humanin vivofluorescence microimaging of the alveolar ducts and sacs during bronchoscopy

Lung elastic fibres from embryonic to adult birds

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