Three-Dimensional Otic Neuronal Progenitor Spheroids Derived from Human Embryonic Stem Cells

Heuer, Rachel A.; Nella, Kevin T.; Chang, Hsiang-Tsun; Coots, Kyle S.; Oleksijew, Andrew M.; Roque, Christian B.; Silva, Luisa Helena Andrade; McGuire, Tammy L.; Homma, Kazuaki; Matsuoka, Akihiro

doi:10.1089/ten.tea.2020.0078

Cited by 9 publications

(6 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To facilitate the inner ear transplantation through the round window, wt-ONPs and hi-ONPs were initially arranged in three-dimensional spheroids (Additional file 1 : Fig. S4A), as previously described [ 18 , 19 ]. Spheroids were transplanted into the left cochlea, while the right cochlea served as a control.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Enhanced survival of hypoimmunogenic otic progenitors following intracochlear xenotransplantation: repercussions for stem cell therapy in hearing loss models

et al. 2023

Self Cite

View full text Add to dashboard Cite

Stem cell replacement holds the potential for sensorineural hearing loss (SNHL) treatment. However, its translation into clinical practice requires strategies for improving stem cell survival following intracochlear transplantation. Considering recent findings showing that the inner ear contains a resident population of immune cells, we hypothesized that immune evasion would improve the survival and residence time of transplanted stem cells in the cochlea, potentially leading to better outcomes. To test this, we leveraged genetic engineering techniques to develop hypoimmunogenic human-induced pluripotent stem cells (hi-iPSC), which lack human leukocyte antigen expression. We found that gene editing does not affect the biological properties of hi-iPSCs, including their capacity to differentiate into otic neural progenitors (ONPs). Compared to wild-type ONPs, more hypoimmunogenic ONPs (derived from hi-iPSCs) were found in the inner ear of immunocompetent mice ten days following cochlear xenotransplantation. This approach may open a new avenue for experimental and clinical SNHL treatments.

show abstract

Section: Resultsmentioning

confidence: 99%

“…Seven days before transplantation, wt-ONPs and hi-ONPs were arranged in three-dimensional spheroids, as previously described [ 18 , 19 ]. The spheroids were then inserted into the left cochlea of immunocompetent C57BL/6 mice, through the round window (Additional file 1 : Fig.…”

Section: Methodsmentioning

confidence: 99%

Enhanced survival of hypoimmunogenic otic progenitors following intracochlear xenotransplantation: repercussions for stem cell therapy in hearing loss models

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

“…The AFM-like soft engagement enables the establishment of reliable organoid-diaphragm contact—vital for achieving a high signal-to-noise ratio. Besides, compared to previous cantilever indentation system 22 , 37 to measure biomechanical properties of organoids, this AFM-like soft engagement approach to immobilize the non-adherent organoids is advantageous. It is fast, convenient, reversible and can be applied to a single organoid in a natural culture dish condition.…”

Section: Resultsmentioning

confidence: 99%

A soft and ultrasensitive force sensing diaphragm for probing cardiac organoids instantaneously and wirelessly

et al. 2022

View full text Add to dashboard Cite

Time-lapse mechanical properties of stem cell derived cardiac organoids are important biological cues for understanding contraction dynamics of human heart tissues, cardiovascular functions and diseases. However, it remains difficult to directly, instantaneously and accurately characterize such mechanical properties in real-time and in situ because cardiac organoids are topologically complex, three-dimensional soft tissues suspended in biological media, which creates a mismatch in mechanics and topology with state-of-the-art force sensors that are typically rigid, planar and bulky. Here, we present a soft resistive force-sensing diaphragm based on ultrasensitive resistive nanocracked platinum film, which can be integrated into an all-soft culture well via an oxygen plasma-enabled bonding process. We show that a reliable organoid-diaphragm contact can be established by an ‘Atomic Force Microscope-like’ engaging process. This allows for instantaneous detection of the organoids’ minute contractile forces and beating patterns during electrical stimulation, resuscitation, drug dosing, tissue culture, and disease modelling.

show abstract

“…al demonstrated that these hESC-derived ONP spheroids are more conducive to survival and differentiation in transplantation by micropipette. In their study, the spheroids maintained characteristics similar to ONPs, which are precursors to SGNs, thereby demonstrating that this procedure is viable for transplantation of stem cells within the inner ear [26].…”

Section: Embryonic Stem Cell (Esc) Therapymentioning

confidence: 86%

The Use Of Stem Cells In The Treatment Of Sensorineural Hearing Loss

Keller¹,

Gallicchio²

2021

Journal of Stem Cell Research

View full text Add to dashboard Cite

Hearing loss affects millions of Americans in every age group, and it can be either conductive or sensorineural. Sensorineural hearing loss (SNHL) specifically has etiologies including trauma, infectious conditions, ototoxin exposure, and trauma. This type of hearing loss affects the conversion of mechanical sound to an electrical signal within the inner ear, and profound SNHL can usually only be treated with a cochlear implant. Neural stem cells (NSCs), embryonic stem cells (ESCs), mesenchymal stem cells (MSCs), and induced pluripotent stem cells (iPSCs) are all promising candidates for the treatment of SNHL. They have the potential to be induced to differentiate into sensory hair cells and spiral ganglion neurons (SGNs) in damaged cochleae. However, more research is still needed to improve the protocol for differentiating stem cells into hair cells and SGNs.

show abstract

Three-Dimensional Otic Neuronal Progenitor Spheroids Derived from Human Embryonic Stem Cells

Cited by 9 publications

References 59 publications

Enhanced survival of hypoimmunogenic otic progenitors following intracochlear xenotransplantation: repercussions for stem cell therapy in hearing loss models

Enhanced survival of hypoimmunogenic otic progenitors following intracochlear xenotransplantation: repercussions for stem cell therapy in hearing loss models

A soft and ultrasensitive force sensing diaphragm for probing cardiac organoids instantaneously and wirelessly

The Use Of Stem Cells In The Treatment Of Sensorineural Hearing Loss

Contact Info

Product

Resources

About