Dissolved organic nitrogen (DON) is the dominant form of fixed nitrogen in most low and middle latitude ocean surface waters. Here, we report measurements of DON isotopic composition (δ 15 N) from the west South China Sea (SCS), with the goal of providing new insight into DON cycling. The concentration of DON in the surface ocean is correlated (r = 0.70, p < 0.0001) with chlorophyll a concentration, indicating DON production in these surface waters. The concentration and δ 15 N of DON fall in a relatively narrow range in the surface ocean (4.6 ± 0.1 μM and 4.3 ± 0.2‰ vs. air, respectively; ±SD), similar to other ocean regions. The mean DON δ 15 N above 50 m (4.5 ± 0.3‰) is similar to the δ 15 N of nitrate in the "shallow subsurface" (i.e., immediately below the euphotic zone; 4.6 ± 0.2‰) but is higher than the δ 15 N of suspended particles in the surface ocean (~2.3‰). This set of isotopic relationships has been observed previously (e.g., in the oligotrophic North Atlantic and North Pacific) and can be explained by the cycling of N between particulate organic nitrogen (PON), DON, and ammonium, in which an isotope effect associated with DON degradation preferentially concentrates 15 N in DON. Consistent with this view, a negative correlation (r = 0.70) between the concentration and the δ 15 N of DON is observed in the upper 75 m, suggesting an isotope effect of~4.9 ± 0.4‰ for DON degradation. Comparing the DON δ 15 N data from the SCS with other regions, we find that the δ 15 N difference between euphotic zone DON and shallow subsurface nitrate δ 15 N (Δδ 15 N (DON-NO3)) rises from ocean regions of inferred net DON production to regions of net DON consumption, with the SCS representing an intermediate case. Plain Language Summary In most areas of the (sub)tropical surface ocean, dissolved inorganic nitrogen (e.g., nitrate) is fully consumed by phytoplankton as a critical nutrient in their growth. In contrast, the concentration of dissolved organic nitrogen (DON) in these waters is 1-3 orders of magnitude higher than dissolved inorganic nitrogen. The high concentration of DON in oligotrophic oceans is often explained as a result of its chemical recalcitrance, which prevents it from being rapidly assimilated and used by plankton. However, the concentration of DON does vary, and it may play an important role in upper ocean N cycle. In this study, DON in the South China Sea (SCS) was investigated for its concentration and natural isotopic composition (i.e., its ratio of heavier 15 N relative to lighter 14 N). We find evidence for both production and consumption of DON in the shallow waters of the SCS. Our findings thus support the possibility that DON is an important part of the upper ocean N cycle. In addition, a comparison of the SCS DON data with those from other ocean regions is consistent with previous suggestions of lateral transfer of DON from regions of upwelling and vertical mixing to lower-nutrient, more stratified tropical and subtropical waters.