Three-Dimensional Printed Design of Antibiotic-Releasing Esophageal Patches for Antimicrobial Activity Prevention

Kim, Seong Dong; Kim, In Gul; Tran, Hao Nguyen; Cho, Hanna; Janarthanan, Gopinathan; Noh, Insup; Chung, Eun‐Jae

doi:10.1089/ten.tea.2020.0268

Cited by 11 publications

(8 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The device used in this study is the upgraded version of our previously reported twin screw system for homogeneous mixing and bioprinting. [ 8 ] The high shear in the TSE system may cause damage to the gel network causing loss in gel viscosity and strength. As the shear rate increases with the rpm, gel viscosity and strength were determined at different screw rpm to find the working zone of the device with this gel.…”

Section: Resultsmentioning

confidence: 99%

“…The MTT assay was then done according to the previous protocol. [8,22] The optical density (OD) values were measured at the wavelength of 570 nm and the relative cell viability (%) standardized to the day 1 control was reported.…”

Section: In Vitro Cytotoxicity Assay (Mtt Assay)mentioning

confidence: 99%

“…To regulate the biological and structural features of the printed tissue engineering construct, the 3D bioprinting of an advanced multicomponent bioink demands the homogenous distribution of bioink components, leading to the controlled release of drugs from the fabricated gels. [ 8 ] To ensure the uniform mixing of micro/nanomaterials and other components in a bioink, we have introduced a one‐step approach by designing a semi‐automated twin‐screw extruder (TSE) head that performs simultaneous mixing and 3D bioprinting of multicomponent advanced bioink with minimal cell damages. [ 9 ] The designed TSE extruder head has proved to disperse the nanoparticles efficiently in a short time.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

High Molecular Weight Fucoidan Loading Into and Release from Hyaluronate‐Based Prefabricated Hydrogel and its Nanogel Particles Controlled by Variable Pitch and Differential Extensional Shear Technology of Advanced Twin Screw‐Based System

Khatun

Bhattacharyya

Taheri

et al. 2022

Adv Materials Technologies

Self Cite

View full text Add to dashboard Cite

Efficient incorporation of high molecular weight drug into prefabricated hydrogel and its delivery are big challenges in its applications to drug delivery and tissue regeneration using a 3D bioprinting system. In this work, a new method of loading a model high molecular weight drug (fucoidan with 200 kDa) into prefabricated hyaluronate‐based terpolymeric gel is developed by using an advanced screw‐based extrusion printing system. This system effectively incorporates the fucoidan biomolecules into the prefabricated crosslinked gel within 60 s at significantly higher percentages without much change in gel properties. Less damage in gel network is achieved through variable pitch and differential extensional shear mechanism by optimization of bioprinting conditions. Importantly, fucoidan‐loaded nanoparticles (NPs) are developed using the advanced extrusion system at high screw rpm and with increased residence time using recirculation. The encapsulation efficiency, sustained release of fucoidan, and in vitro cell culture studies confirm the nontoxic nature of the drug‐loaded gels and nanogels at lower doses, exhibiting the biomedical application potentials of this advanced screw‐based extrusion printing system and drug‐loaded NPs formation in pharmaceuticals, 3D bioprinting, tissue engineering as well as maximal drug loading into and sustained release from prefabricated gels.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: In Vitro Cytotoxicity Assay (Mtt Assay)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

High Molecular Weight Fucoidan Loading Into and Release from Hyaluronate‐Based Prefabricated Hydrogel and its Nanogel Particles Controlled by Variable Pitch and Differential Extensional Shear Technology of Advanced Twin Screw‐Based System

Khatun

Bhattacharyya

Taheri

et al. 2022

Adv Materials Technologies

Self Cite

View full text Add to dashboard Cite

show abstract

“…In previous studies, we tested polymeric scaffolds with various structures (eg. 3D printing and electrospinning) for 3D esophageal reconstruction [5][6][7][8]. Among them, it was confirmed that the strong elasticity of polyurethane (PU) electrospun nanofiber provide the most appropriate physical properties to withstand esophageal peristalsis [5,9].…”

Section: Introductionmentioning

confidence: 93%

Assessment of Esophageal Reconstruction via Bioreactor Cultivation of a Synthetic Scaffold in a Canine Model

Kim¹,

Wu²,

Choi³

et al. 2023

Clin Exp Otorhinolaryngol

Self Cite

View full text Add to dashboard Cite

Objectives: The use of tissue-engineered materials for esophageal reconstruction is a technically challenging task in animal and requires bioreactor training for enhancing cellular reactivity. Recently, there have been many attempts at esophageal tissue engineering, but the success rate has been limited due to difficulty in initial epithelialization in the special environment of peristalsis. The purpose of this study was to evaluate the potential of an artificial esophagus that can enhance the regeneration of esophageal mucosa and muscle through the optimal combination of a double-layered polymeric scaffold and a custom-designed MSC-based bioreactor system in canine model.Methods: We fabricated a novel double-layered scaffold as a tissue-engineered esophagus using an electrospinning technique. Prior to transplantation, human-derived mesenchymal stem cells were seeded into the lumen of the scaffold, and bioreactor cultivation was performed to enhance cellular reactivity. After 3 days of cultivation using a bioreactor system, tissue-engineered artificial esophagus was transplanted into a partial esophageal defect (5 x 3 cm-long resection) in a canine model. Results: From the SEM analysis, electrospun fibers in a tubular scaffold were found to be randomly and circumferentially oriented toword the inner and outer surfaces, respectively. Complete recovery of the esophageal mucosa was confirmed by endoscopic analysis and SEM. Esophagogastroduodenoscopy (EGD) and CT analysis also showed that there were no signs of leakage or stricture and that there were normal lumen with complete epithelialization. Significant regeneration of the mucosal layer was observed by keratin 5 immunostaining. From the

show abstract

“…The cytotoxicity study for the MPLE gel was evaluated according to a previous protocol reported from our labs [43][44][45]. Brie y, the round disk-shaped gel samples (500 µl, diameter = 10 mm) were prepared by spreading the gel on a glass coverslip and then rinsing it in a petri dish containing 10 ml of distilled water for 5 days by replacing the water daily.…”

Section: In Vitro Cytotoxicity Assaysmentioning

confidence: 99%

Control of maleic acid-propylene diepoxide hydrogel for 3D printing application for flexible tissue engineering scaffold with high resolution by end capping and graft polymerization

Tran

Kim

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

Background Control of 3D printing of highly tough hydrogel inks with adequate printability, scaffold fidelity and mechanical properties are highly desirable for biomedical and tissue engineering applications. However, developing a biocompatible tough ink with high-resolution printability, biodegradability, adhesion, and integration with surrounding tissues is a big challenge in 3D printing. The aim of this study was to develop extrusion-based 3D printing of viscous hydrogel composing of maleic acid and propylene diepoxide by controlling continuous mechanisms of condensation and radical polymerization. Methods The molecular weight of highly adhesive propagating poly(malate-co-propylene oxide) copolymer was controlled by capping its growing chain with mono-functional lipoic acid during condensation reaction to form lipoic acid capped gel (LP-capped gel). Poly(ethylene oxide)-diacrylate, PEGDA, is graft-polymerized to the LP-capped backbone polymer (MPLE gel) by UV irradiation during 3D printing process to control the properties of printability, cell adhesiveness and fidelity with high resolution and mechanical properties (MPLE scaffold). Both the highly adhesive LP-capped gel and printing-controlled MPLE gel/scaffolds are diversely characterized and compared with for their applications to the extrusion-based printability, including biocompatibility, self-healing, drug releasing, adhesiveness, multi-layered high-resolution printing, and in vitro/in vivo tests. Results The ratio at 1:0.3 (w/v) between LP-capped gel and PEGDA was chosen for the optimal results for 3D printing at high resolution (90–140µm in strut thickness) with various complex geometries (lattice, rhombus, and honeycomb). In addition, the long-term release profiles of bioactive molecules were well-controlled by incorporating high molecular BSA (21 days, 98.4 ± 0.69%), or small molecule ORN (14 days, 97.1 ± 1.98%) into the MPLE gel scaffolds for the tests of potential therapeutic applications. More importantly, the MPLE gels represents excellent in vitro cyto-compatibility against osteoblast-like cells (MC3T3) with viability value at 96.43% ± 7.48% over 7 culturing days. For in-vivo studies, the flexible MPLE scaffolds showed significant improvement on angiogenesis with minor inflammatory response after 4-week implantation in mice. Conclusion The MPLE gel inks was well-controlled for the fabrication of flexible complex tissue engineering scaffold with high resolutions, shear-thinning and post-printing fidelity, by modulating the composition of the highly adhesive LP-capped gel and inert PEGDA as well as end capping of lipoic acid to the propagating poly(malate-co-propylene oxide) copolymer.

show abstract

Three-Dimensional Printed Design of Antibiotic-Releasing Esophageal Patches for Antimicrobial Activity Prevention

Cited by 11 publications

References 38 publications

High Molecular Weight Fucoidan Loading Into and Release from Hyaluronate‐Based Prefabricated Hydrogel and its Nanogel Particles Controlled by Variable Pitch and Differential Extensional Shear Technology of Advanced Twin Screw‐Based System

High Molecular Weight Fucoidan Loading Into and Release from Hyaluronate‐Based Prefabricated Hydrogel and its Nanogel Particles Controlled by Variable Pitch and Differential Extensional Shear Technology of Advanced Twin Screw‐Based System

Assessment of Esophageal Reconstruction via Bioreactor Cultivation of a Synthetic Scaffold in a Canine Model

Control of maleic acid-propylene diepoxide hydrogel for 3D printing application for flexible tissue engineering scaffold with high resolution by end capping and graft polymerization

Contact Info

Product

Resources

About