Three‐dimensional reconstruction of the guinea pig inner ear, comparison of OPFOS and light microscopy, applications of 3D reconstruction

Hofman, Rutger; Segenhout, J. M.; Wit, H. P.

doi:10.1111/j.1365-2818.2009.03115.x

Cited by 24 publications

(25 citation statements)

References 23 publications

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“…Additionally, we measured the length of the 8.5 cochlear half-turns (including the hook region) on a XY projection of the cochlear spiral that was derived from 3D reconstruction data of the guinea pig inner ear (Fig. 4b, adapted from [32]; reprinted with the kind permission from the corresponding author R. Hofman and the publisher Wiley - Blackwell ). The results of the width and length measurements determined in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Data on the baso-apical length of the cochlear spiral of the adult guinea pig were derived from Hofman et al [32] and used to determine the surface area of RM and the OC. In their original description, Hofman et al [32] described the length of full cochlear turns, but not of each cochlear half-turn.…”

Section: Methodsmentioning

confidence: 99%

“…In their original description, Hofman et al [32] described the length of full cochlear turns, but not of each cochlear half-turn. Therefore, an XY projection of the cochlear spiral derived from 3D reconstruction data of the guinea pig cochlea was used to measure the individual longitudinal lengths of the eight cochlear half-turns and the hook region.…”

Section: Methodsmentioning

confidence: 99%

“…As P D and P f are defined as the transport flux of water per unit membrane area [19], surface areas of the entire CDE, RM and the OC were quantified from histological sections and previously derived morphological data of cochlear fluid space dimensions from the adult guinea pig cochlea [32]. The membrane area of complementary AQP expression in OSCs of the cochlear apex was determined by measurements of immunohistochemically labelled sections of the adult guinea pig cochlea.…”

Section: Introductionmentioning

confidence: 99%

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Water permeability of the mammalian cochlea: functional features of an aquaporin-facilitated water shunt at the perilymph–endolymph barrier

Eckhard

Müller

Salt

et al. 2014

Pflugers Arch - Eur J Physiol

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The cochlear duct epithelium (CDE) constitutes a tight barrier that effectively separates the inner ear fluids, endolymph and perilymph, thereby maintaining distinct ionic and osmotic gradients that are essential for auditory function. However, in vivo experiments have demonstrated that the CDE allows for rapid water exchange between fluid compartments. The molecular mechanism governing water permeation across the CDE remains elusive. We computationally determined the diffusional (PD) and osmotic (Pf) water permeability coefficients for the mammalian CDE based on in silico simulations of cochlear water dynamics integrating previously derived in vivo experimental data on fluid flow with expression sites of molecular water channels (aquaporins, AQPs). The PD of the entire CDE (PD = 8.18 × 10−5 cm s−1) and its individual partitions including Reissner's membrane (PD = 12.06 × 10−5 cm s−1) and the organ of Corti (PD = 10.2 × 10−5 cm s−1) were similar to other epithelia with AQP-facilitated water permeation. The Pf of the CDE (Pf = 6.15 × 10−4 cm s−1) was also in the range of other epithelia while an exceptionally high Pf was determined for an epithelial subdomain of outer sulcus cells in the cochlear apex co-expressing AQP4 and AQP5 (OSCs; Pf = 156.90 × 10−3 cm s−1). The Pf/PD ratios of the CDE (Pf/PD = 7.52) and OSCs (Pf/PD = 242.02) indicate an aqueous pore-facilitated water exchange and reveal a high-transfer region or “water shunt” in the cochlear apex. This “water shunt” explains experimentally determined phenomena of endolymphatic longitudinal flow towards the cochlear apex. The water permeability coefficients of the CDE emphasise the physiological and pathophysiological relevance of water dynamics in the cochlea in particular for endolymphatic hydrops and Ménière's disease.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Water permeability of the mammalian cochlea: functional features of an aquaporin-facilitated water shunt at the perilymph–endolymph barrier

Eckhard

Müller

Salt

et al. 2014

Pflugers Arch - Eur J Physiol

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“…A comparison between OPFOS imaging and physical sectioning was performed with guinea pigs, but the procedures were not performed on the same cochleae and 2-hydroxypropyl methacrylate was used instead of celloidin 15 . Therefore this study proposes to assess the compatibility of histological sectioning after the same specimen has been imaged with TSLIM.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Traditional Histology and TSLIM Optical Sectioning of Human Temporal Bones

Johnson¹,

Cüreoğlu²,

O’Malley³

et al. 2014

Otology &Amp; Neurotology

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Hypothesis Thin-sheet laser imaging microscopy (TSLIM) optical sectioning can be used to assess temporal bone soft tissue morphology before celloidin sectioning. Background Traditional human temporal bone (TB) celloidin embedding and sectioning is a lengthy and involved process. Although bone morphology can be assessed with microCT prior to traditional histology, soft tissue structures are difficult to resolve until after celloidin sectioning. A potential solution is TSLIM, a high resolution, non-destructive optical sectioning technique first developed to image bone and soft tissue in animal cochleae. Methods Two temporal bones from one individual were used to evaluate TSLIM’s capacity to image human temporal bones (bone and soft tissue) before traditional histology. The right TB was trimmed to the cochlea, prepared for and imaged with TSLIM, then processed for celloidin sectioning. The left TB, serving as a control, was directly prepared for traditional celloidin sectioning. Results TSLIM imaging of the right TB showed adequate resolution of all major tissue structures, but barely resolved cells. Celloidin sections produced from the TSLIM-imaged right TB were equivalent in cytological detail to those from the traditionally prepared left TB. TSLIM three-dimensional (3D) reconstructions were superior to those obtained from celloidin sections because TSLIM produced many more sections that were without mechanical sectioning artifacts or alignment issues. Conclusion TSLIM processing disturbs neither gross nor detailed morphology and integrates well with celloidin histology making it an ideal method to image soft tissue before celloidin sectioning.

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Light‐Sheet Applications: From Rare Cell Detection to Full Organ Analysis

Colombelli,

Tosi,

Maizel

et al. 2024

Light Sheet Fluorescence Microscopy

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Three‐dimensional reconstruction of the guinea pig inner ear, comparison of OPFOS and light microscopy, applications of 3D reconstruction

Cited by 24 publications

References 23 publications

Water permeability of the mammalian cochlea: functional features of an aquaporin-facilitated water shunt at the perilymph–endolymph barrier

Water permeability of the mammalian cochlea: functional features of an aquaporin-facilitated water shunt at the perilymph–endolymph barrier

Comparison of Traditional Histology and TSLIM Optical Sectioning of Human Temporal Bones

Light‐Sheet Applications: From Rare Cell Detection to Full Organ Analysis

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