Three dimensional simulation on binding efficiency of immunoassay for a biosensor with applying electrothermal effect

Huang, Kuan-Rong; Chang, Jeng‐Shian

doi:10.1007/s00231-013-1214-z

Cited by 27 publications

(34 citation statements)

References 22 publications

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“…Biological buffers such as PBS, Britton–Robinson and Carmody buffers have been used as carrier in different ACET studies . ACET firstly was investigated by Antonio Ramos et al.…”

Section: Introductionmentioning

confidence: 99%

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Bi‐directional ACET micropump for on‐chip biological applications

et al. 2016

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The ability to control and pump high ionic strength fluids inside microchannels forms a major advantage for clinical diagnostics and drug screening processes, where high conductive biological and physiological buffers are used. Despite the known potential of AC electro-thermal (ACET) effect in different biomedical applications, comparatively little is known about controlling the velocity and direction of fluid inside the chip. Here, we proposed to discretize the conventional electrodes to form various asymmetric electrode structures in order to control the fluid direction by simple switching the appropriate electric potential applied to the discretized electrodes. The ACET pumping effect was numerically studied by solving electrical, thermal and hydrodynamic multi-physic coupled equations to optimize the geometrical dimensions of the discretized system. PBS solutions with different ionic strength were seeded with 1 μm sized fluorescent particles and electrothermally driven fluid motion was observed inside the channel for different electrode structures. Experimental analyses confirm that the proposed micropump is efficient for a conductivity range between 0.1 and 1 S/m and the efficiency improves by increasing the voltage amplitude. Behavior of the proposed electrode-electrolyte system is discussed by lumped circuit model. Frequency response of system illustrated that the optimal frequency range increases by increasing the conductivity of medium. For 0.18 S/m PBS solution, the constant pumping effect was observed at frequency range between 100 kHz and 1 MHz, while frequency range of 100 kHz to 5 MHZ was observed for 0.42 S/m. The characteristics of experimental results were in good agreement with the theoretical model.

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“…Biological buffers such as PBS, Britton–Robinson and Carmody buffers have been used as carrier in different ACET studies . ACET firstly was investigated by Antonio Ramos et al.…”

Section: Introductionmentioning

confidence: 99%

“…ACET flow was implemented for instance to improve the binding efficiency of on‐chip immunoassay biosensors . Numerical study of ACET flow was proposed as actuation mechanism for mixing applications, with the same size of electrode pairs on the opposite side walls of a microchannel .…”

Section: Introductionmentioning

confidence: 99%

Bi‐directional ACET micropump for on‐chip biological applications

et al. 2016

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“…The analyte is transported to reach the sensitive layer, that could be described by the following equation [38]:…”

Section: The Governing Equations and Problem Set Upmentioning

confidence: 99%

“…Compared to the inlet flow velocity [13] as a conduction system, we get an improvement concerning the initial slope of the association phase of binding reaction from 1.8 × 10 −11 to 2.29 × 10 −11 . Recently, many attempts were made to improve such microfluidic transport system by superimposing another enhancement structure, namely, the use of the electrothermal force effect [38], the use of an obstacle above the biosensor [47], the confinement flow effect [14] and mixing electrothermal and confinement effect together [48].…”

Section: Angular Velocity Effect On the Binding Reactionmentioning

confidence: 99%

2D simulation of a microfluidic biosensor for CRP detection into a rotating micro-channel

et al. 2019

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Previously, pumping systems using centrifugal force has been regarded as an attractive control method for fluid flow interior microchannel. In this work, we propose a novel design of a biosensor for complex reactive protein detection in two-dimensional rotating microchannel using the finite element method. We have first developed the physical modeling of the centrifugal force driven, and detailed velocity profile and expended the pressure distribution. Several crucial factors that influence the equilibrium binding time are discussed, including the bulk analyte concentration and the biosensor length. The obtained analytical results reveal that the binding reaction is largely enhanced with the increase of the angular velocity. Hence, an appropriate choice of the angular velocity may enhance the microfluidic biosensor performance and reduce considerably the time response. This work can open new opportunity for further investigation in microflow injection experimental studies.

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“…The procedure of numerical simulation for the electrothermal force was shown in many literatures 5,33 and our previous works. 45,46 Since our purpose in numerical simulation was to view the local vortex flow field and temperature field near the electrodes created by ETE, a simplified 3-D block including only two pair of microelectrodes in the ETE-QCM chip was adopted, as shown in Figure 3(a). The dimensions of the block are 1500 lm Â 500 lm Â 150 lm.…”

Section: Numerical Simulationsmentioning

confidence: 99%

Improving the binding efficiency of quartz crystal microbalance biosensors by applying the electrothermal effect

et al. 2014

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A quartz crystal microbalance (QCM) serving as a biosensor to detect the target biomolecules (analytes) often suffers from the time consuming process, especially in the case of diffusion-limited reaction. In this experimental work, we modify the reaction chamber of a conventional QCM by integrating into the multimicroelectrodes to produce electrothermal vortex flow which can efficiently drive the analytes moving toward the sensor surface, where the analytes were captured by the immobilized ligands. The microelectrodes are placed on the top surface of the chamber opposite to the sensor, which is located on the bottom of the chamber. Besides, the height of reaction chamber is reduced to assure that the suspended analytes in the fluid can be effectively drived to the sensor surface by induced electrothermal vortex flow, and also the sample costs are saved. A series of frequency shift measurements associated with the adding mass due to the specific binding of the analytes in the fluid flow and the immobilized ligands on the QCM sensor surface are performed with or without applying electrothermal effect (ETE). The experimental results show that electrothermal vortex flow does effectively accelerate the specific binding and make the frequency shift measurement more sensible. In addition, the images of the binding surfaces of the sensors with or without applying electrothermal effect are taken through the scanning electron microscopy. By comparing the images, it also clearly indicates that ETE does raise the specific binding of the analytes and ligands and efficiently improves the performance of the QCM sensor. V C 2014 AIP Publishing LLC. [http://dx

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Three dimensional simulation on binding efficiency of immunoassay for a biosensor with applying electrothermal effect

Cited by 27 publications

References 22 publications

Bi‐directional ACET micropump for on‐chip biological applications

Bi‐directional ACET micropump for on‐chip biological applications

2D simulation of a microfluidic biosensor for CRP detection into a rotating micro-channel

Improving the binding efficiency of quartz crystal microbalance biosensors by applying the electrothermal effect

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