Three-Dimensional Stable Alginate-Nanocellulose Gels for Biomedical Applications: Towards Tunable Mechanical Properties and Cell Growing

Siqueira, Priscila; Siqueira, Éder José; Lima, Ana Elza de; Siqueira, Gilberto; Pinzón-García, Ana Delia; Lopes, Ana Paula; Cortés, María Esperanza; Isaac, Augusta; Pereira, Fabiano Vargas; Botaro, Vagner Roberto

doi:10.3390/nano9010078

Cited by 102 publications

(74 citation statements)

References 65 publications

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“…This result is similar to a previously reported study using alginate-CNC hydrogel, where it has been determined that incorporating CNC to pure alginate increases the porosity of hydrogel [52]. A similar finding reported that the addition of CNF to alginate increases the pore size of hydrogel more than the addition of CNC to alginate [70]. Post degradation, the porous structure area reduces to 500 ± 350 μm 2 for 2A4CNC and 1200 ± 700 μm 2 for 4A1CNF.…”

Section: Evaluation Of Degradationsupporting

confidence: 91%

Shape Fidelity of 3D-Bioprinted Biodegradable Patches

Temirel

Hawxhurst

2021

Micromachines

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There is high demand in the medical field for rapid fabrication of biodegradable patches at low cost and high throughput for various instant applications, such as wound healing. Bioprinting is a promising technology, which makes it possible to fabricate custom biodegradable patches. However, several challenges with the physical and chemical fidelity of bioprinted patches must be solved to increase the performance of patches. Here, we presented two hybrid hydrogels made of alginate-cellulose nanocrystal (CNC) (2% w/v alginate and 4% w/v CNC) and alginate-TEMPO oxidized cellulose nanofibril (T-CNF) (4% w/v alginate and 1% w/v T-CNC) via ionic crosslinking using calcium chloride (2% w/v). These hydrogels were rheologically characterized, and printing parameters were tuned for improved shape fidelity for use with an extrusion printing head. Young’s modulus of 3D printed patches was found to be 0.2–0.45 MPa, which was between the physiological ranges of human skin. Mechanical fidelity of patches was assessed through cycling loading experiments that emulate human tissue motion. 3D bioprinted patches were exposed to a solution mimicking the body fluid to characterize the biodegradability of patches at body temperature. The biodegradation of alginate-CNC and alginate-CNF was around 90% and 50% at the end of the 30-day in vitro degradation trial, which might be sufficient time for wound healing. Finally, the biocompatibility of the hydrogels was tested by cell viability analysis using NIH/3T3 mouse fibroblast cells. This study may pave the way toward improving the performance of patches and developing new patch material with high physical and chemical fidelity for instant application.

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Section: Evaluation Of Degradationsupporting

confidence: 91%

Shape Fidelity of 3D-Bioprinted Biodegradable Patches

Temirel

Hawxhurst

2021

Micromachines

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“…Probably, the nanocelluloses influenced the water holding capacity, related to the second water loss event. [ 28 ] Also, a slight change in the T max was observed, when compared to CMC.…”

Section: Resultsmentioning

confidence: 99%

Nanocellulose as Reinforcement in Carboxymethylcellulose Superabsorbent Nanocomposite Hydrogels

Lima

Souza

Rosa

2020

Macromolecular Symposia

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Carboxymethylcellulose (CMC) is an abundant and low‐cost material that can be used to synthesize environmentally friendly superabsorbent hydrogels for numerous applications. However, this biopolymer results in hydrogels with poor mechanical properties; different fillers can be used to improve this property, but occasionally can decrease the swelling capacity. Nanocelluloses (NC) are an excellent option to improve hydrogel physicochemical characteristics. In this work, CMC‐hydrogels are prepared using citric acid, and NC is added as a filler in different contents: 1, 3, and 5 wt%. The Fourier Transformed Infrared spectroscopy (FTIR) is used to confirm the crosslink reaction, and a peak related to the carboxylic acid functional group confirmed the hydrogel formation. The NC incorporation didn't change the FTIR spectrum since both materials have a similar structure. The water absorption analysis indicated that the developed can be classified as superabsorbent since all of them show an absorption capacity above 40 g.g−1. Besides that, NC played an essential role in the water absorption capacity, the incorporation of 3% of NC resulted in a material with a degree of swelling of, approximately 100 g.g−1. The NCs act as an excellent filler, improving the storage modulus significantly, i.e., the mechanical resistance of the material. The developed hydrogels showed exceptional characteristics, being adequate to various applications, mainly agricultural ones aiming for the water delivery to the soil.

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“…Recent literature mentioned that nanostructured hydrogels can facilitate the biological functions and tissue growth by stimulating interactions between cells and their environment 64 . The morphology of the hydrogels was evaluated by SEM analysis (Fig.…”

Section: Discussionmentioning

confidence: 99%

Therapeutic effects of antibiotics loaded cellulose nanofiber and κ-carrageenan oligosaccharide composite hydrogels for periodontitis treatment

Johnson

Kong

Miao

et al. 2020

Sci Rep

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Periodontitis is an inflammatory disease that can lead to the periodontal pocket formation and tooth loss. This study was aimed to develop antimicrobials loaded hydrogels composed of cellulose nanofibers (CNF) and κ-carrageenan oligosaccharides (CO) nanoparticles for the treatment of periodontitis. Two antimicrobial agents such as surfactin and Herbmedotcin were selected as the therapeutic agents and the hydrogels were formulated based on the increasing concentration of surfactin. The proposed material has high thermal stability, controlled release, and water absorption capacity. This study was proceeded by investigating the in vitro antibacterial and anti-inflammatory properties of the hydrogels. This material has strong antibacterial activity against periodontal pathogens such as Streptococcus mutans, Porphyromonas gingivalis, Fusobacterium nucleatum, and Pseudomonas aeruginosa. Moreover, a significant increase in malondialdehyde (MDA) production and a decrease in biofilm formation and metabolic activity of the bacteria was observed in the presence of hydrogel. Besides, it reduced the reactive oxygen species (ROS) generation, transcription factor, and cytokines production in human gingival fibroblast cells (HGF) under inflammatory conditions. In conclusion, the hydrogels were successfully developed and proven to have antibacterial and anti-inflammatory properties for the treatment of periodontitis. Thus, it can be used as an excellent candidate for periodontitis treatment.

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Three-Dimensional Stable Alginate-Nanocellulose Gels for Biomedical Applications: Towards Tunable Mechanical Properties and Cell Growing

Cited by 102 publications

References 65 publications

Shape Fidelity of 3D-Bioprinted Biodegradable Patches

Shape Fidelity of 3D-Bioprinted Biodegradable Patches

Nanocellulose as Reinforcement in Carboxymethylcellulose Superabsorbent Nanocomposite Hydrogels

Therapeutic effects of antibiotics loaded cellulose nanofiber and κ-carrageenan oligosaccharide composite hydrogels for periodontitis treatment

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