1990
DOI: 10.1126/science.2377893
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Three-Dimensional Structure of Cellobiohydrolase II from Trichoderma reesei

Abstract: The enzymatic degradation of cellulose is an important process, both ecologically and commercially. The three-dimensional structure of a cellulase, the enzymatic core of CBHII from the fungus Trichoderma reesei reveals an alpha-beta protein with a fold similar to but different from the widely occurring barrel topology first observed in triose phosphate isomerase. The active site of CBHII is located at the carboxyl-terminal end of a parallel beta barrel, in an enclosed tunnel through which the cellulose threads… Show more

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Cited by 590 publications
(430 citation statements)
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“…5) acts as a proton donor in general acid catalysis [5]. Unexpectedly, this residue was not conserved in the amino acid sequence first reported for Streptomyces KSM-9 CasA (see [lo] and Fig.…”
Section: S U L~~e -H O N D E D P E P T I~e~~~r O M the Cena And Cexmentioning
confidence: 99%
See 2 more Smart Citations
“…5) acts as a proton donor in general acid catalysis [5]. Unexpectedly, this residue was not conserved in the amino acid sequence first reported for Streptomyces KSM-9 CasA (see [lo] and Fig.…”
Section: S U L~~e -H O N D E D P E P T I~e~~~r O M the Cena And Cexmentioning
confidence: 99%
“…4). These data allowed unambiguous assignment of the two disulfide bonds of the CenA catalytic domain as Cys248 -Cys291 and Cys390 - 5) shows that these bonds correspond to two disulfide bonds previously assigned to the catalytic domain of T. reesei CBH I1 (family B) [5]. Four peptides, T3 -T6, from the non-reduced digest of p33 (Cex catalytic domain) were chosen for further analyses (Fig.…”
Section: Dimvide Bond Assignments For Cena and Cexmentioning
confidence: 99%
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“…The overall structure of AD2 PF-ChiA is a TIM-barrel ( / ) 8 -fold with a tunnel-like active site (Fig. 2) that is a common feature of exo-chitinases (Davies & Henrissat, 1995;Spezio et al, 1993;Rouvinen et al, 1990). Chitinase B from S. marcescens (PDB code 1e15) and chitinase 1 from Coccidioides immitis (PDB code 1d2k) were retrieved from the family 18 chitinases with the highest Z scores (19.8 and 18.8, respectively) using the DALI server (http://www.ebi.ac.uk/dali/; Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Thus the catalytic domain of GA is not a trivial variant of the o/p-barrel, observed in a-and Pamylases (Matsuura et al, 1984;Buisson et al, 1987;Boel et al, 1990;Mikami et al, 1992Mikami et al, , 1993, cyclodextrin glucanosyltransferases (Hofinann et al, 1989;Kubota et al, 1990;Klein and Schulz, 1991), cellobiohydrolases (Rouvinen et al, 1990), and predicted for a large N-lcrin…”
Section: Table Of Contentsmentioning
confidence: 99%