Three-dimensional tissue culture model of human breast cancer for the evaluation of multidrug resistance

Ding, Yanfang; Liu, Wei; Yu, Wenwu; Lu, Shenzhou; Liu, Ming; Kaplan, David L.; Wang, Xiuli

doi:10.1002/term.2729

Cited by 22 publications

(16 citation statements)

References 35 publications

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“…Hydrogels have been employed in numerous biomedical applications, namely, as scaffolds (Rossi, Santoro, & Perale, ) and controlled release systems (Grijalvo, Mayr, Eritja, & Díaz, ). Additionally, hydrogels can be used as 3D cell culture models to study the cellular behaviour or optimize the dose of pharmacological‐based therapies (Ding et al, ). Hydrogel networks contain a high content of water and a porous structure, allowing the exchange of gas, nutrients, biomolecules, and waste with their surrounding environment.…”

Section: Introductionmentioning

confidence: 99%

Phospholipid‐induced silk fibroin hydrogels and their potential as cell carriers for tissue regeneration

Laomeephol

Guedes

Ferreira

et al. 2019

J Tissue Eng Regen Med

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Silk fibroin (SF) hydrogels can be obtained via self-assembly, but this process takes several days or weeks, being unfeasible to produce cell carrier hydrogels. In this work, a phospholipid, namely, 1,2-dimyristoyl-sn-glycero-3-phospho-(1′-rac-glycerol) sodium salt (DMPG), was used to induce and accelerate the gelation process of SF solutions. Due to the amphipathic nature and negative charge of DMPG, electrostatic and hydrophobic interactions between the phospholipids and SF chains will occur, inducing the structural transition of SF chains to the beta sheet and consequently a rapid gel formation is observed (less than 50 min). Moreover, the gelation time can be controlled by varying the lipid concentration. To assess the potential of the hydrogels as cell carriers, several mammalian cell lines, including L929, NIH/3T3, SaOS-2, and CaSki, were encapsulated into the hydrogel. The silk-based hydrogels supported the normal growth of fibroblasts, corroborating their cytocompatibility.Interestingly, an inhibition in the growth of cancer-derived cell lines was observed.Therefore, DMPG-induced SF hydrogels can be successfully used as a 3D platform for in situ cell encapsulation, opening promising opportunities in biomedical applications, such as in cell therapies and tissue regeneration.Bombyx mori "Nangnoi Srisaket 1" Thai silk cocoons were obtained from Queen Sirikit Sericulture Center, Srisaket province, Thailand.DMPG and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) were purchased from Avanti Lipids Polar, USA. Cell culture medium and all other reagents were purchased from Thermo Fisher Scientific.All reagents were of analytical grade. | SF solution preparation and its zeta potential determinationSF solution was prepared using the method adapted from Kim, Park, Joo Kim, Wada, and Kaplan (2005). In brief, silk cocoons were boiled in 0.02 M Na 2 CO 3 for 20 min, followed by their thoroughly washing with distilled water. The extracted silk fibre was then dissolved in 9.3 M LiBr at 60°C for 4 hr. The silk solution was dialysed against distilled water using a dialysis tube (MWCO 12-16 kDa; Sekisui, Japan) for 48 hr. The final concentration was approximately 6%, which was determined from dried solid weight.Analysis of zeta potential of the SF solution was conducted using laser doppler electrophoresis in a Zetasizer Nano ZS equipment (Malvern Instruments) at 25°C.

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Section: Introductionmentioning

confidence: 99%

Phospholipid‐induced silk fibroin hydrogels and their potential as cell carriers for tissue regeneration

Laomeephol

Guedes

Ferreira

et al. 2019

J Tissue Eng Regen Med

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“…In particular, we described cancer models engineered to study a variety of tumor types, including those developing in the skin, bladder, and eye tissues. Other important cancer types for which solid 3D tumor models are being developed by various teams include prostate and breast cancers [20,[233][234][235][236][237][238][239][240]. Recent advances for these tumor types include the use of organoids and the incorporation of patient-derived samples (blood derivatives, cells, and/or ECM).…”

Section: Perspectives and Future Directionsmentioning

confidence: 99%

Human Organ‐Specific 3D Cancer Models Produced by the Stromal Self‐Assembly Method of Tissue Engineering for the Study of Solid Tumors

Roy

Magne

Vaillancourt-Audet

et al. 2020

BioMed Research International

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Cancer research has considerably progressed with the improvement of in vitro study models, helping to understand the key role of the tumor microenvironment in cancer development and progression. Over the last few years, complex 3D human cell culture systems have gained much popularity over in vivo models, as they accurately mimic the tumor microenvironment and allow high-throughput drug screening. Of particular interest, in vitrohuman 3D tissue constructs, produced by the self-assembly method of tissue engineering, have been successfully used to model the tumor microenvironment and now represent a very promising approach to further develop diverse cancer models. In this review, we describe the importance of the tumor microenvironment and present the existing in vitro cancer models generated through the self-assembly method of tissue engineering. Lastly, we highlight the relevance of this approach to mimic various and complex tumors, including basal cell carcinoma, cutaneous neurofibroma, skin melanoma, bladder cancer, and uveal melanoma.

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“…In comparison to 2D models, MDA‐MB‐231 cells were less sensitive in 3D due to restricted diffusion because of the physical structure of the scaffold (Talukdar & Kundu, 2012) (studies summarized in Table 3). Also utilizing a naturally derived silk platform, Ding et al (2018) sought to elucidate signaling pathways and other biological factors that contribute to drug resistance in breast cancer. The study found that the DOX‐resistant MCF7 cell line exhibited greater drug efflux due to overexpression of P‐gp and other ABC transporters, along with glutathione conjugation and altered cycle distribution.…”

Section: Scaffold‐based Modelsmentioning

confidence: 99%

“…The study found that the DOX‐resistant MCF7 cell line exhibited greater drug efflux due to overexpression of P‐gp and other ABC transporters, along with glutathione conjugation and altered cycle distribution. The altered cell cycle distribution increased the population of BCSCs (Ding et al, 2018), which has been reported to induce drug resistance (Charafe‐Jauffret et al, 2010). This study also observed the presence of a greater population of dormant cells outlasting treatment, in comparison to 2D culture (Ding et al, 2018).…”

Section: Scaffold‐based Modelsmentioning

confidence: 99%

“…The altered cell cycle distribution increased the population of BCSCs (Ding et al, 2018), which has been reported to induce drug resistance (Charafe‐Jauffret et al, 2010). This study also observed the presence of a greater population of dormant cells outlasting treatment, in comparison to 2D culture (Ding et al, 2018). Another natural polymer used to study drug resistance via the scaffold model is agarose.…”

Section: Scaffold‐based Modelsmentioning

confidence: 99%

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Three‐dimensional culture models to study drug resistance in breast cancer

Fisher

Rao

2020

Biotech & Bioengineering

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Despite recent advances in breast cancer treatment, drug resistance frequently presents as a challenge, contributing to a higher risk of relapse and decreased overall survival rate. It is now generally recognized that the extracellular matrix and cellular heterogeneity of the tumor microenvironment influences the cancer cells' ultimate fate. Therefore, strategies employed to examine mechanisms of drug resistance must take microenvironmental influences, as well as genetic mutations, into account. This review discusses three‐dimensional (3D) in vitro model systems which incorporate microenvironmental influences to study mechanisms of drug resistance in breast cancer. These bioengineered models include spheroid‐based models, biomaterial‐based models such as polymeric scaffolds and hydrogels, and microfluidic chip‐based models. The advantages of these model systems over traditionally studied two‐dimensional tissue culture polystyrene are examined. Additionally, the applicability of such 3D models for studying the impact of tumor microenvironment signals on drug response and/or resistance is discussed. Finally, the potential of such models for use in the development of strategies to combat drug resistance and determine the most promising treatment regimen is explored.

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Three-dimensional tissue culture model of human breast cancer for the evaluation of multidrug resistance

Cited by 22 publications

References 35 publications

Phospholipid‐induced silk fibroin hydrogels and their potential as cell carriers for tissue regeneration

Phospholipid‐induced silk fibroin hydrogels and their potential as cell carriers for tissue regeneration

Human Organ‐Specific 3D Cancer Models Produced by the Stromal Self‐Assembly Method of Tissue Engineering for the Study of Solid Tumors

Three‐dimensional culture models to study drug resistance in breast cancer

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