2023
DOI: 10.1021/acschemneuro.2c00807
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Three-Dimensional Tracking of Intracellular Calcium and Redox State during Real-Time Control in a Hypoxic Gradient in Microglia Culture: Comparison of the Channel Blocker and Reoxygenation under Ischemic Shock

Abstract: Real-time three-dimensional (3-D) imaging is crucial for quantifying correlations among various molecules under acute ischemic stroke. Insights into such correlations may be decisive in selecting molecules capable of providing a protective effect within a shorter period. The major bottleneck is maintaining the cultures under severely hypoxic conditions while simultaneously 3-D imaging intracellular organelles with a microscope. Moreover, comparing the protective effect of drugs and reoxygenation remains challe… Show more

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Cited by 3 publications
(1 citation statement)
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“…Despite the use of machine learning methods for functional clustering, effectively grouping heterogeneous responses remains challenging (Muldoon et al, 2013). Such clustering approaches, based on Ca 2+ spiking features, although capable of segregating the cell population into subgroups, do not offer mechanistic insights into these identified clusters (Dhyani et al, 2023;Yao et al, 2016). In contrast, biophysical modeling and parameter estimation allow the depiction of receptor, neurotransmitter, and transporter levels, thereby defining the cell state signature and communication levels.…”
mentioning
confidence: 99%
“…Despite the use of machine learning methods for functional clustering, effectively grouping heterogeneous responses remains challenging (Muldoon et al, 2013). Such clustering approaches, based on Ca 2+ spiking features, although capable of segregating the cell population into subgroups, do not offer mechanistic insights into these identified clusters (Dhyani et al, 2023;Yao et al, 2016). In contrast, biophysical modeling and parameter estimation allow the depiction of receptor, neurotransmitter, and transporter levels, thereby defining the cell state signature and communication levels.…”
mentioning
confidence: 99%