2020
DOI: 10.1101/2020.05.29.118034
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Three-dimensional understanding of the morphological complexity of the human uterine endometrium

Abstract: The histological basis of the human uterine endometrium has been established by 2D observation. However, the fundamental morphology of endometrial glands is not sufficiently understood because these glands have complicated winding and branching patterns. To construct a big picture of endometrial gland structure, we performed tissue-clearing-based 3D imaging of human uterine endometrial tissue. Our 3D immunohistochemistry and 3D layer analyses revealed that endometrial glands formed a plexus network in the stra… Show more

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Cited by 7 publications
(14 citation statements)
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“…Histologic examination of this case showed a rather superficial adenomyosis within 20% of myometrial thickness, probably extending directly from the overlying basal endometrium where the LCM was performed. This finding supports the view that direct ingrowth from the horizontally interconnected endometrial glands and associated stroma in basalis [40] results in early adenomyosis [41]. Those epithelial and stromal progenitor cells in adenomyosis then develop independently from the adjacent endometrium basalis once they reside in the myometrium, similar to the development of peritoneal endometriosis, as proposed in a previous study [42].…”
supporting
confidence: 89%
“…Histologic examination of this case showed a rather superficial adenomyosis within 20% of myometrial thickness, probably extending directly from the overlying basal endometrium where the LCM was performed. This finding supports the view that direct ingrowth from the horizontally interconnected endometrial glands and associated stroma in basalis [40] results in early adenomyosis [41]. Those epithelial and stromal progenitor cells in adenomyosis then develop independently from the adjacent endometrium basalis once they reside in the myometrium, similar to the development of peritoneal endometriosis, as proposed in a previous study [42].…”
supporting
confidence: 89%
“…N-cadherin + cells were localized to the apical and lateral surfaces of the epithelial cells and rarely colocalized with the basalis epithelial marker nuclear SOX9. It is possible that the N-cadherin + cells are located on horizontal branching and rhizome like glandular structures recently identified in the basalis of human endometrium (Tempest et al, 2020;Yamaguchi et al, 2020).…”
Section: Surface Markers Of Human Endometrial Epithelial Stem/progenitor Cellsmentioning
confidence: 99%
“…It is also not known whether ciliated cells are part of the putative endometrial epithelial hierarchy. Nor is it clear how this epithelial hierarchy are located in 3D endometrium, given the degree of gland branching and rhizome formation in the basalis glands (Tempest et al, 2020;Yamaguchi et al, 2020).…”
Section: Endometrial Epithelial Stem/progenitor Cell Hierarchymentioning
confidence: 99%
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“…Endoglandular trophoblasts invade the maternal uterine glands and thus enable ow of their glandular secretion products into the intervillous space of the placenta. Other than hitherto assumed, the uterine glands within the endometrium are no singular tubes, instead they have branches and connections and even form a plexus network within the stratum basalis and expand along the myometrium [154]. Although demonstrated so far only in non-pregnant endometrium, it is unlikely that this plexus network vanishes in the decidua with the beginning of pregnancy rather than persisting as a glandular network throughout (at least) early pregnancy.…”
Section: C) the Endoglandular Evt Populationmentioning
confidence: 90%