Three-dimensional whole body imaging of spin probes in mice by time-domain radiofrequency electron paramagnetic resonance

Afeworki, Mobae; Dam, Gooitzen M. van; Devasahayam, Nallathamby; Murugesan, Ramachandran; Cook, John A.; Coffin, Deborah; A.-Larsen, Jan H.; Mitchell, James B.; Subramanian, S.; Krishna, Murali C.

doi:10.1002/(sici)1522-2594(200003)43:3<375::aid-mrm9>3.0.co;2-g

Cited by 57 publications

(50 citation statements)

References 25 publications

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“…38 Over the last decade the NCI group has made significant improvements in the electronics and resonator designs, enabling this technique to be useful for performing functional imaging in whole animals and tissues. 16,[39][40][41] However, FT EPR imaging is currently limited to a few narrow-line spin probes.…”

Section: Cw Vs Pulse Imagingmentioning

confidence: 99%

“…Another major advantage in the pulse method is that it is insensitive to object motion, thus making it possible to study living animals that may cause random as well as periodic cardiac and respiratory motions. 16 The two methods differ widely in the choice of probes that can be used. The pulse method is limited to narrow line probes, with linewidth of the order of 100 mG or less.…”

Section: Cw Vs Pulse Imagingmentioning

confidence: 99%

“…3,6 During the last few years significant progress has been made to enable the performance of EPR imaging of biological samples with improved image modality, resolution and quality to obtain useful information. [9][10][11][12][13][14][15][16][17][18] Over the last decade we have focused on developing instrumentation optimized for measurements of free radicals in isolated beating hearts, and in recent several years we have extended our efforts to develop hardware and software to enable spatial and spectral-spatial imaging of free radicals in the heart. 9,[19][20][21][22][23][24][25] This review provides a summary of the development and application of the imaging techniques with examples including imaging of stable free radical labels, oxygen concentrations and nitric oxide generation in the normal or ischemic heart.…”

Section: Introductionmentioning

confidence: 99%

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Cardiac applications of EPR imaging

Kuppusamy

Zweíer

2004

NMR in Biomedicine

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This review summarizes the development and application of a variety of EPR imaging modalities including spatial, spectral-spatial (spectroscopic), gated-imaging and oxygen mapping to cardiovascular studies. It has been hypothesized that free radical metabolism, oxygenation and nitric oxide generation in biological organs such as the heart may vary over the spatially defined tissue structure. We have developed instrumentation optimized for 3D spatial and 3D or 4D spectral-spatial imaging of free radicals at 1.2 GHz. Using this instrumentation high quality 3D spectral-spatial imaging of nitroxyl (nitroxide) metabolism was performed, as well as spatially localized measurements of oxygen concentrations, based on the oxygen-dependent line-broadening of the EPR spectrum. Both exogenously infused probes and endogenous radicals were used to obtain the images. It is demonstrated that the EPR imaging is a powerful tool which can provide unique information regarding the spatial localization of free radicals, oxygen and nitric oxide in biological organs and tissues.

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Section: Cw Vs Pulse Imagingmentioning

confidence: 99%

Section: Cw Vs Pulse Imagingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cardiac applications of EPR imaging

Kuppusamy

Zweíer

2004

NMR in Biomedicine

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“…13 Trityl distribution images of a whole mouse have also been acquired by use of free induction decay (FID)-based time-domain EPR imaging. 14,15 In the case of ESE imaging, one also has to face the issue of the frequency bandwidth of the imager transmitter and receiver arms. Applied field gradients encode the object's spatial information into signal frequency offsets.…”

Section: Introductionmentioning

confidence: 99%

Frequency bandwidth extension by use of multiple Zeeman field offsets for electron spin-echo EPR oxygen imaging of large objects

et al. 2011

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Purpose: Electron spin-echo (ESE) oxygen imaging is a new and evolving electron paramagnetic resonance (EPR) imaging (EPRI) modality that is useful for physiological in vivo applications, such as EPR oxygen imaging (EPROI), with potential application to imaging of multicentimeter objects as large as human tumors. A present limitation on the size of the object to be imaged at a given resolution is the frequency bandwidth of the system, since the location is encoded as a frequency offset in ESE imaging. The authors' aim in this study was to demonstrate the object size advantage of the multioffset bandwidth extension technique. Methods: The multiple-stepped Zeeman field offset (or simply multi-B) technique was used for imaging of an 8.5-cm-long phantom containing a narrow single line triaryl methyl compound (trityl) solution at the 250 MHz imaging frequency. The image is compared to a standard single-field ESE image of the same phantom. Results: For the phantom used in this study, transverse relaxation (T 2e ) electron spin-echo (ESE) images from multi-B acquisition are more uniform, contain less prominent artifacts, and have a better signal to noise ratio (SNR) compared to single-field T 2e images. Conclusions: The multi-B method is suitable for imaging of samples whose physical size restricts the applicability of the conventional single-field ESE imaging technique.

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“…If the sensitive volume of an RF resonator is slice-selective, 2D EPR imaging with such an RF resonator could be useful for obtaining quasi-slice EPR images. Recently, pulsed EPR imagers at a low-magnetic field have been developed for small rodents [23][24][25]. However, pulsed EPR techniques in animal experiments are limited to spin probes that have longer relaxation times, e.g., currently on the order of microseconds.…”

Section: Introductionmentioning

confidence: 99%

A loop resonator for slice-selective in vivo EPR imaging in rats

Hirata

Deng

et al. 2008

Journal of Magnetic Resonance

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A loop resonator was developed for 300-MHz continuous-wave electron paramagnetic resonance (CW-EPR) spectroscopy and imaging in live rats. A single-turn loop (55 mm in diameter) was used to provide sufficient space for the rat body. Efficiency for generating a radiofrequency magnetic field of 38 µT/W 1/2 was achieved at the center of the loop. For the resonator itself, an unloaded quality factor of 430 was obtained. When a 350 g rat was placed in the resonator at the level of the lower abdomen, the quality factor decreased to 18. The sensitive volume in the loop was visualized with a bottle filled with an aqueous solution of the nitroxide spin probe 3-carbamoyl-2,2,5,5-tetramethyl-3-pyrrolin-1-yloxy (3-CP). The resonator was shown to enable EPR imaging in live rats. Imaging was performed for 3-CP that had been infused intravenously into the rat and its distribution was visualized within the lower abdomen.

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Three-dimensional whole body imaging of spin probes in mice by time-domain radiofrequency electron paramagnetic resonance

Cited by 57 publications

References 25 publications

Cardiac applications of EPR imaging

Cardiac applications of EPR imaging

Frequency bandwidth extension by use of multiple Zeeman field offsets for electron spin-echo EPR oxygen imaging of large objects

A loop resonator for slice-selective in vivo EPR imaging in rats

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