Three-iron-sulfur centers of pea mitochondria

Burbaev, D.Sh.; Vakhnina, L.V.; Zoz, N. N.

doi:10.1016/0005-2728(88)90004-7

Cited by 2 publications

(6 citation statements)

References 8 publications

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“…The shape of the ESR signat and g-factor of the new center ate close to those of [3Fe-4S] centers and its relaxation characteristics are close to those of the [3Fe-4S] center of aconitase and those found in plant mitochondria [8,10]. The detected center has the ESR signal in sufficiently oxidative states in the cell.…”

Section: Results and Diseussionmentioning

confidence: 82%

“…2). In addition, the specific line shape, g-factor and the sharp temperature dependence (the spin lattice interaction is so strong that the spectrum is broad and the intensity of the ESR signal is negligibly srnall at 34 K [8][9][10] I I I I I I I I I I I 3300 3400 3500 3600 3700 Magnetlc Fie]d (G) is present in the respiratory chain of some bacteria [3,13,14]. However, as the exact role of this dehydrogenase in the metabolism of MR-1 is still unknown, we will refer to these centers as ISC-1 and ISC-2, until further investigation establishes that this block of centers belongs to the same metabolic enzyme.…”

Section: Results and Diseussionmentioning

confidence: 99%

“…Thus, compared to center ISC-2, center ISC-3 does not become reduced when succinate is added, i.e., this center has lower effective redox potential. Plant mitochondria also have an [3Fe-4S] center which remains in the oxidized state when the chain is reduced with succinate [8].…”

Section: Results and Diseussionmentioning

confidence: 99%

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Studies of the iron-sulphur centers of the bacteriaShewanella putrefaciens (MR-1)

Tsapin¹,

Burbaev²,

Nealson

et al. 1994

Appl. Magn. Reson.

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Abstraet. Shewanella putrefaciens can be grown using any one of several different terminal acceptors. This respiratory versatility stimulated our studies of the respiratory chain of MR-1 using whole cell analysis by ESR spectroscopy. Initial analyses of aerobically grown cells showed the presence of the succinate dehydrogenase complex. Also, it was shown that in S. putrefaciens which were grown anaerobically with iron as the only electron acceptor the ESR signal at g = 2.021 appeared at low tempemtures. This ESR signal resembled the ESR signal of [Fe-S] center of succinate dehydrogenase in oxidized state. However this new ESR signal hada different temperature dependence. It can be seen at temperature as high as 40 K. The line shape and g-factor of the new center ore very similar to those of [3Fe-4S] centers. As this ESR signal can be seen only in the anaerobic samples grown with iron as acceptor we suggest that this center is located in the terminal part of the respiratory chain and is associated with the reduction of iron or another electron acceptor.

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Section: Results and Diseussionmentioning

confidence: 82%

Section: Results and Diseussionmentioning

confidence: 99%

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Studies of the iron-sulphur centers of the bacteriaShewanella putrefaciens (MR-1)

Tsapin¹,

Burbaev²,

Nealson

et al. 1994

Appl. Magn. Reson.

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“…It should be mentioned that the coinciding g factors for FR-1 and S-1 have been previously noted for E. coli preparations as well [8,22]. After oxidation of preparations of MR-1 cells grown with lactate and fumarate, it was possible to record at low temperature the intensive narrow ESR signal at g --2.020 (the line shape was the same as in axial symmetry center, which is characteristic for FR-3 [22] and S-3 [12,15,16] (see also Fig. 5).…”

Section: Resultsmentioning

confidence: 87%

“…The conditions of MR-7 growth (aerobically with succinate as the electron donor) and strong spinlattice interaction of the center (the ESR signal is so broadened at 25-30 K, that its intensity is very low (see Fig. 2) permit one to unambiguously identify this center as S-3 [15,16].…”

Section: Resultsmentioning

confidence: 99%

Investigations of succinate dehydrogenase and fumarate reductase in whole cells ofShewanella putrefaciens (strains MR-1 and MR-7) using electron spin resonance spectroscopy

Tsapin

Burbaev²,

Nealson

et al. 1995

Appl. Magn. Reson.

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Using ESR spectroscopy we were able to detect succinate dehydrogenase (SDH) in the whole cells of Shewanella putrefaciens grown aerobically. ESR analysis of cells grown under anaerobic conditions in the presence of fumarate as electron acceptor revealed signals with the same parameters as SDH. However the temperattwe dependence was quite different. Furthermore, the addition of sodium azide led to disappearance of this ESR signal in anaerobic cultures, while the same treatment of aerobic cultures had no effect on the ESR signal of SDH. Based on these results, we postulate that the ESR signal from the anaerobic cells is that of fumarate reductase, similar in its properties to the fumarate reductase from Escherichia coli.

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Three-iron-sulfur centers of pea mitochondria

Cited by 2 publications

References 8 publications

Studies of the iron-sulphur centers of the bacteriaShewanella putrefaciens (MR-1)

Studies of the iron-sulphur centers of the bacteriaShewanella putrefaciens (MR-1)

Investigations of succinate dehydrogenase and fumarate reductase in whole cells ofShewanella putrefaciens (strains MR-1 and MR-7) using electron spin resonance spectroscopy

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