Three isotypes of immunoglobulin light chains in large yellow croaker, Pseudosciaena crocea: Molecular cloning, characterization, and expression analysis

Molecular characterization and expression analysis of four isotypes of immunoglobulin light chain genes in orange-spotted grouper, Epinephelus coioides

“…All four C L isotypes could be detected in multiple organs, but expression was most abundant in the lymphoid tissues, head-kidney and spleen. Similar results were previously obtained using Northern blotting or quantitative PCR for rainbow trout L1 and L2, common carp L1A, L1B and L3, Atlantic salmon IgL types 1 to 3, fugu IgL, emerald rockcod L1A, L1B, L2 and L3, channel catfish Igr and Igk, and large yellow croaker L1, L2 and L3 (Coscia et al, 2008;Edholm et al, 2009;Saha et al, 2004;Solem and Jørgensen, 2002;Tian et al, 2011;Timmusk et al, 2000;Tomana et al, 2002). Our analyses also indicated that OsgIgL is expressed in the gill, an important mucosal organ which contains a variety of leukocytes and produces secretory Igs (Salinas Fig.…”

“…et al, 2011). Furthermore, OsgIgL expression was also detected in non-lymphoid areas, such as the brain, eye, heart, liver and muscle, which might arise from the remains of peripheral blood lymphocytes (Tian et al, 2011).…”

“…The conserved characteristic FR4 (J L ) motif FGXGT exists in all V L types of OsgIgL in one of two forms, FGGGT (V L -I, II, III and IV) or FGQGT (V L -V); in OsgIgH, however, the consensus sequence is WGKGT. Moreover, the FR4 sequence Phe-Gly-Gln-Gly-Thr-LysLeu-Ile-Val (FGQGTKLIV) was observed in the V L -V type OsgIgL, and other r type IgLs in rainbow trout (Oncorhynchus mykiss), common carp (Cyprinus carpio), emerald rockcod (Trematomus bernacchii) and large yellow croaker (Pseudosciaena crocea) (Coscia et al, 2008;Ishikawa et al, 2004;Tian et al, 2011;Timmusk et al, 2000). The two V regions from IgH and IgL compose the antigen binding site surface of Ig molecules, to which three CDR loops from each V domain contribute.…”

Preferential combination between the light and heavy chain isotypes of fish immunoglobulins

“…The four IgL isotypes were expressed abundantly in the lymphoid tissues of trout, which was similar with that obtained from carp L1a and L1b (Tomana et al, 2002), salmon L1, L2 and L3 (Solem and Jorgensen, 2002), catfish IgLl and IgLs (Edholm et al, 2009), orange-spotted grouper CL-I to CL-IV (Wu et al, 2013), large yellow croaker (Pseudosciaena crocea) IgL1 to IgL3 (Tian et al, 2011). Besides, the transcripts of trout IgLs were also detected in the non-lymphoid tissues, such as heart and liver, which might be due to the remain of peripheral blood lymphocytes in these tissues.…”

“…In large yellow croaker, all three IgL isotypes, especially IgL1, could be stimulated by poly (I:C) or inactivated trivalent bacterin (Tian et al, 2011). The expression of the four isotypes of grouper IgL was also observed under stimulation of LPS, poly (I:C) and GIV (grouper iridovirus), with each isotype showing differential expression pattern in a time-dependent manner (Wu et al, 2013).…”