Three mutations identified in the voltage-sensitive sodium channel α-subunit gene of permethrin-resistant human head lice reduce the permethrin sensitivity of house fly Vssc1 sodium channels expressed in Xenopus oocytes

SupYoon, Kyong; Symington, Steven B.; Lee, Si Hyeock; Soderlund, David M.; Clark, J. Marshall

doi:10.1016/j.ibmb.2007.11.011

Cited by 68 publications

(53 citation statements)

References 46 publications

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“…As suggested for head lice, under the selective pressure of permethrin, the mutations M815I and L920F are the first to take place. This event would be followed by the onset of the T917I mutation (12), which is considered the main cause of this resistance, while the M815I and L920F mutations would reduce permethrin sensitivity (29). These findings suggest that the population of body lice infesting homeless people in Marseille has been subjected to selective pressure.…”

Section: Discussionmentioning

confidence: 97%

Detection of a Knockdown Resistance Mutation Associated with Permethrin Resistance in the Body Louse Pediculus humanus corporis by Use of Melting Curve Analysis Genotyping

et al. 2012

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Louse-borne diseases are prevalent in the homeless, and body louse eradication has thus far been unsuccessful in this population. We aim to develop a rapid and robust genotyping method usable in large field-based clinical studies to monitor permethrin resistance in the human body louse Pediculus humanus corporis. We assessed a melting curve analysis genotyping method based on real-time PCR using hybridization probes to detect the M815I-T917I-L920F knockdown resistance (kdr) mutation in the paraorthologous voltage-sensitive sodium channel (VSSC) ␣ subunit gene, which is associated with permethrin resistance. The 908-bp DNA fragment of the VSSC gene, encoding the ␣ subunit of the sodium channel and encompassing the three mutation sites, was PCR sequenced from 65 lice collected from a homeless population. We noted a high prevalence of the 3 indicated mutations in the body lice collected from homeless people (100% for the M815I and L920F mutations and 56.73% for the T917I mutation). These results were confirmed by melting curve analysis genotyping, which had a calculated sensitivity of 100% for the M815I and T917I mutations and of 98% for the L920F mutation. The specificity was 100% for M815I and L920F and 96% for T917I. Melting curve analysis genotyping is a fast, sensitive, and specific tool that is fully compatible with the analysis of a large number of samples in epidemiological surveys, allowing the simultaneous genotyping of 96 samples in just over an hour (75 min). Thus, it is perfectly suited for the epidemiological monitoring of permethrin resistance in human body lice in large-scale clinical studies.T he human body louse Pediculus humanus corporis (P. h. corporis) is a hematophagous ectoparasite that lives and multiplies in clothing. Body lice are vectors of 3 major infectious diseases: epidemic typhus caused by Rickettsia prowazekii, relapsing fever caused by Borrelia recurrentis, and trench fever caused by Bartonella quintana (24). The poor living conditions and crowded situations in homeless, war refugee, or natural disaster victim populations provide ideal conditions for the spread of lice (23). Body louse infestation has been observed in 22% of the sheltered homeless population in Marseille, France, causing trench fever, epidemic typhus, and relapsing fever (4), and coinfestation with head lice is often noted. Consequently, all measures that can be used to decrease the burden of these ectoparasites in homeless people are warranted to avoid the spread and/or outbreak of these diseases. Clothing change as well as the use of ivermectin to eradicate the body lice in a cohort of homeless individuals in Marseille was unsuccessful (10), suggesting that the complete eradication of this ectoparasite is a true challenge (1, 5). It was therefore essential for us to investigate other strategies to reach this goal.During the 1980s, a study conducted by the U.S. Army demonstrated that the use of uniforms impregnated with 0.125 mg/ cm 2 permethrin effectively eradicated body lice (28). The resistance to permethrin, ...

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Section: Discussionmentioning

confidence: 97%

Detection of a Knockdown Resistance Mutation Associated with Permethrin Resistance in the Body Louse Pediculus humanus corporis by Use of Melting Curve Analysis Genotyping

et al. 2012

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“…Sequence analyses of cloned cDNA fragments and genomic DNA fragments from individual louse samples confirmed that all the mutations existed en bloc as a resistant haplotype. Further experiments using site-directed mutagenesis at the corresponding amino acid sequence positions of the house fly paraorthologous VGSC a-subunit (Vssc1WT ) gene and heterologous co-expression with the sodium channel auxiliary subunit of house fly (Vsscb) in Xenopus oocytes 13 showed that the T917I mutation was the principal cause of permethrin resistance in head lice via a kdr-type nerve insensitivity mechanism.…”

Section: Introductionmentioning

confidence: 99%

Permethrin and malathion resistance in head lice: Results of ex vivo and molecular assays

Bouvresse

Berdjane

Durand

et al. 2012

Journal of the American Academy of Dermatology

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“…In the resistant head louse colonies, four amino acid substitutions were identiÞed, all of which were conÞrmed in three resistant head louse colonies collected from Tokyo (Tomita et al 2003a, b). Recently, an electrophysiological study showed that three of the abovementioned point mutations (i.e., M815I, T929I, and L932 F) are associated with pyrethroid resistance in head louse (Yoon et al 2008). All combinations of these mutations were inserted into the sodium channel gene of houseßy and expressed in Xenopus oocytes with the sodium channel auxiliary subunit of houseßy, Vssc␤.…”

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confidence: 99%

Prevalence ofkdr-like Mutations Associated with Pyrethroid Resistance in Human Head Louse Populations in Japan

et al. 2009

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Pyrethroid insecticides play very important roles in the control of pediculosis, which is caused by human head louse (Pediculus humanus capitis De Geer) worldwide. The development of head louse resistance to pyrethroid pediculicides has become a global issue during the last two decades. Pyrethroid resistance is associated with amino acid substitutions in the alpha-subunit of the para-sodium channel gene; these substitutions are termed kdr-like mutations. The frequencies of four mutations (D11E, M815I, T929I, and L932F) in the sodium channel gene were studied in human head louse colonies collected from Japan by using SNaPshot method. A total of 630 head lice collected from 282 infested people were analyzed, and it was found that 55 lice of 19 colonies homozygously or heterozygously possessed kdr-like genes, in which the four mutations existed concomitantly. This suggested that the quadruple mutant haplotype is a common feature of the kdr genes of pandemic head lice. The frequencies of the occurrence of resistant genes in the total individuals tested and resistant gene-carrying colonies were 8.7 (55/630) and 6.7% (19/263), respectively. Because the resistant gene was detected in the colonies collected from 11 of the 22 prefectures surveyed, it is speculated that resistant head lice are already spread extensively in Japan. This was the first large-scale survey of pyrethroid resistant head lice in Japan.

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Three mutations identified in the voltage-sensitive sodium channel α-subunit gene of permethrin-resistant human head lice reduce the permethrin sensitivity of house fly Vssc1 sodium channels expressed in Xenopus oocytes

Cited by 68 publications

References 46 publications

Detection of a Knockdown Resistance Mutation Associated with Permethrin Resistance in the Body Louse Pediculus humanus corporis by Use of Melting Curve Analysis Genotyping

Detection of a Knockdown Resistance Mutation Associated with Permethrin Resistance in the Body Louse Pediculus humanus corporis by Use of Melting Curve Analysis Genotyping

Permethrin and malathion resistance in head lice: Results of ex vivo and molecular assays

Prevalence ofkdr-like Mutations Associated with Pyrethroid Resistance in Human Head Louse Populations in Japan

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