Three new species of Culex (Melanoconion) (Diptera: Culicidae) from French Guiana based on morphological and molecular data

Talaga, Stanislas; Gendrin, Mathilde

doi:10.11646/zootaxa.5205.2.5

Cited by 4 publications

(7 citation statements)

References 19 publications

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“…In French Guiana, 245 mosquito species are known [4,5]including 22 species of Anopheles [6] , nine of which belong to the Nyssorhynchus subgenus whose elevation to genus level is under consideration [7][8][9] . Among them,Anopheles darlingi is known to be the main malaria vector in South America [10,11] and Anopheles aquasalis , although not being incriminated as a vector in French Guiana, is also considered as one of the principal vectors in other neighboring countries on the continent [10,12] .…”

Section: Introductionmentioning

confidence: 99%

A novel mosquito species identification method based on PCR and capillary electrophoresis

Chabanol

Romoli

Talaga

et al. 2023

Preprint

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In the Anopheles genus, various mosquito species are able to transmit Plasmodium parasites responsible for malaria, while others are non-vectors. In an effort to better understand the biology of Anopheles species and to quantify transmission risk in an area, the identification of mosquito species collected on the field is an essential but problematic task. Morphological identification requires expertise, well-preserved specimens and high-quality equipment, and it does not allow any subsequent verification when samples are later used in a destructive treatment. Moreover, it involves physical manipulations that are not compatible with experiments requiring fast sampling and processing of specimens, hence species identification is often based on DNA sequencing of reference genes or region such as the Internal Transcribed Spacer 2 (ITS2) region of nuclear ribosomal DNA. Sequencing ITS2 for numerous samples is costly, but the design of species-specific PCR primers is not always possible when local species diversity is high. Here, we introduce a molecular technique of species identification based on precise determination of ITS2 length combined with a simple visual observation, the color of mosquito hindleg tip. DNA extracted from field-collected Anopheles mosquitoes was amplified with universal Anopheles ITS2 primers and analyzed with a capillary electrophoresis device, which precisely determines the size of the fragments. We defined windows of amplicon sizes combined with fifth hind tarsus color, which allow to discriminate the major Anopheles species found in our collections. We validated our parameters via Sanger sequencing of the ITS2 amplicons. This method can be particularly useful in situations with a moderate species diversity, i.e. when the number of local species is too high to define species-specific primers but low enough to avoid individual ITS2 sequencing. This tool will be of interest to evaluate local malaria transmission risk and this approach may further be implemented for other mosquito genera.

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Section: Introductionmentioning

confidence: 99%

A novel mosquito species identification method based on PCR and capillary electrophoresis

Chabanol

Romoli

Talaga

et al. 2023

Preprint

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“…All analyses were performed in R software (R Core Team 2022) using the “vegan” package. Mitochondrial DNA cytochrome c oxidase subunit I gene ( COI ) barcode of each new species was obtained from holotypes and paratypes as already described in Talaga and Gendrin (2022). Sequences were uploaded to the Barcode of Life Data Systems (BOLD) (Ratnasingham and Hebert 2007) as part of the FGMOS project, which gathers all the barcoding data available on the mosquitoes of French Guiana.…”

Section: Methodsmentioning

confidence: 99%

“…Anatomical nomenclature used was based on Harbach and Knight (1980), as well as current terminology for describing the male genitalia of the subgenus Melanoconion (e.g. Sallum 1993, Talaga andGendrin 2022). The abbreviations used for mosquito genera and subgenera follow Reinert (2009).…”

Section: Methodsmentioning

confidence: 99%

“…Despite their important health and economic burden, mosquitoes also constitute a significant component of the biodiversity of aquatic and terrestrial ecosystems. In French Guiana, mosquitoes have long been studied, in some cases extensively, but the ecology and distribution of the 245 currently known species in the territory remains unclear (Talaga et al 2015, Talaga et al 2021, Talaga and Gendrin 2022). Locally, it has been shown that both the aquatic environment of immatures and the terrestrial environment of adults play a role in this distribution (Talaga et al 2017b).…”

Section: Introductionmentioning

confidence: 99%

“…French Guiana is an overseas department of France (∼84,000 km 2) situated in the northeastern part of the Amazon biome in South America. Amazonian savannas of French Guiana are restricted to the coastal plain (the term “coastal floodplain” was misapplied in Talaga et al 2021, and Talaga and Gendrin 2022), where they are part of a mosaic of landscapes also composed of mangroves, marshes, and different types of forests (Figure 1). Although they only cover 0.22% of the territory, they encompass a significant portion of the department's animal and plant diversity.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Mosquitoes (Diptera: Culicidae) of the Amazonian savannas of French Guiana with a description of two new species

Talaga,

Duchemin

2023

Journal of Vector Ecology

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Using capillary electrophoresis to identify Anopheline species in routine sampling sites

Chabanol,

Romoli,

Talaga

et al. 2024

Ecology and Evolution

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In the Anopheles genus, various mosquito species are able to transmit the Plasmodium parasites responsible for malaria, while others are non‐vectors. In an effort to better understand the biology of Anopheles species and to quantify transmission risk in an area, the identification of mosquito species collected in the field is an essential but problematic task. Morphological identification requires expertise and cannot be checked after processing samples in a destructive treatment, while sequencing of numerous samples is costly. Here, we introduce a method of Species identification via Simple Observation Coupled with Capillary Electrophoresis Technology (SOCCET). This molecular technique of species identification is based on precise determination of ITS2 length combined with a simple visual observation, the colour of mosquito hindleg tip. DNA extracted from field‐collected Anopheles mosquitoes was amplified with universal Anopheles ITS2 primers and analysed with a capillary electrophoresis device, which precisely determines the size of the fragments. We defined windows of amplicon sizes combined with fifth hind tarsus colour, which allows discrimination of the major Anopheles species found in our collections. We validated our parameters via Sanger sequencing of ITS2 amplicons. Using the SOCCET method, we characterised the composition of Anopheles populations in five locations of French Guiana, where we detected a total of nine species. Anopheles braziliensis and Anopheles darlingi were detected in four locations each and represented 13 and 67% of our samples, respectively. The SOCCET method can be particularly useful when working with routine sampling sites with a moderate species diversity, that is, when the number of local species is too high to define species‐specific primers but low enough to avoid individual ITS2 sequencing. This tool will be of interest to evaluate local malaria transmission risk and this approach may be further implemented for other mosquito genera.

show abstract

Three new species of Culex (Melanoconion) (Diptera: Culicidae) from French Guiana based on morphological and molecular data

Cited by 4 publications

References 19 publications

A novel mosquito species identification method based on PCR and capillary electrophoresis

A novel mosquito species identification method based on PCR and capillary electrophoresis

Mosquitoes (Diptera: Culicidae) of the Amazonian savannas of French Guiana with a description of two new species

Using capillary electrophoresis to identify Anopheline species in routine sampling sites

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