Three Pea Seedborne Mosaic Virus Pathotypes from Pea and Lentil Germ Plasm

Abstract

Three pea seedborne mosaic virus later unless otherwise mentioned. pathotypes from pea and lentil germ plasm. Plant Disease 70:783-786. Virus and CIP purification. The method of Yeh and Gonsalves (18) was Three isolates of pea seedborne mosaic virus (PSbMV), P-l and P-4 from pea (Pisum sativum) and used to purify CIP from plants infected L-l from lentil (Lens culinaris) germ plasm accessions, were distinguished by their capacity to infect with isolates P-1 and L-I. The two virus pea genotypes. Resistance in peas was isolate-specific. Resistance to the L-l isolate was associated with bean yellow mosaic virus resistance and also with a delayed reaction to isolate P-4. Several pea isolates were purified according to the germ plasm accessions were resistant to all three isolates. All isolates were infective to 26 genetic method of Dougherty and Hiebert (5) lines of chickpea (Cicer arietinum), a new host, but not to 12 accessions of pigeon pea (Cajanus with some modifications. Infected shoots cajan). Antisera produced against the cytoplasmic inclusion protein induced in peas by P-l and L-I of Ranger pea were harvested and frozen were useful in detecting infections by the indirect ELISA method and were generally more sensitive 24-28 days after inoculation. Tissues than antisera to the viral protein. were homogenized in cold potassium phosphate buffer (0.5M, pH 7.5, with 0.1% Na 2 SO 3 and 0.01 M ethylene

Inheritance of Resistance to a Third Pathotype of Pea Seed-Borne Mosaic Virus in Pisum sativum

“…The frequent association of sbm-4 with sbm-1 and sbm-3 in a number of pea lines from India and Ethiopia suggests linkage. 1 In nature, the host range of PSbMV is very restricted. Infected seeds of peas and lentils are the main sources of local and long distance spread of this virus.…”

“…A culture of PSbMV-P4, available from a previous study, was maintained in Bonneville. 1 This cultivar is known to be resistant to several viruses, including PSbMV-Ll and bean yellow mosaic virus (BYMV). 6 Inoculum was prepared by grinding infected pea leaves with a phosphate buffer (K + ) at pH 8.5.…”

“…Antisera to isolates of PSbMV had been prepared during a previous study. 1 All plants were maintained in an insect-free greenhouse at 25-30°C.…”

“…Serologically, PSbMV-P4 was indistinguishable from PSbMV-ST and PSbMV-L, the other two known pathotypes of the virus, but it could be easily differentiated using resistant and susceptible pea genotypes. 1 Previous studies demonstrated that resistance in the pea to PSbMV is viralpathotype specific. 16 Consequently, for effective control of this virus, it is necessary to utilize all the available genes for resistance.…”

Nucleotide Sequence of the Coat Protein Gene of Pea Seed-borne Mosaic Potyvirus

“…PSbMV pathotype P-1 (Alconero et al, 1986), was purified from infected P. sativum plants using a modification of the procedure published by Reddick & Barnett (1983). Full-length viral RNA was isolated from freshly prepared virus as described by Brakke & van Pelt (1970).…”