Three Sensitive Methods for the Detection of Cytomegalovirus in Lung Tissue of Patients with Interstitial Pneumonitis

Jiwa, Mehdi; Steenbergen, Renske D.M.; Zwaan, F. E.; Kluin, Philippus; Raap, A. K.; Ploeg, M. van der

doi:10.1093/ajcp/93.4.491

Cited by 39 publications

(13 citation statements)

References 18 publications

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“…The levels of sensitivity of ISH and IHC seem to be similar. Although Keh and Gerber (1988), Wu et al (1989) and Cremer and Althoff (1991) pointed out it's usefulness, ISH seems to offer only limited additional advantages and can therefore be useful under special questions (Jiwa et al 1989;Clayton et al 1989).…”

Section: Discussionmentioning

confidence: 99%

Comparison of different methods for CMV detection

1994

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The submandibular glands were investigated in 77 cases of SIDS using histology, immunohistochemistry (IHC), in situ hybridization (ISH) and PCR to detect cytomegalovirus (CMV) inclusion disease. The aim of the investigation was to establish the detection of CMV by PCR as a model for the detection of other types of virus by PCR, especially for the detection of viruses which affect the respiratory system of babies. In 14 cases the CMV detection was possible. Typical changes of duct cells associated with CMV infection were observed in 9 by histology and in 4 further cases by IHC. In comparison to these methods, ISH showed a higher sensitivity but only one more positive case was found. Theoretically the PCR method is more sensitive than ISH and more convenient from the practical aspect. Detection of CMV by PCR was possible in 10 out of 14 positive cases. All PCR positive cases were cases showing an active disease but not latent infection.

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Section: Discussionmentioning

confidence: 99%

Comparison of different methods for CMV detection

1994

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“…Several viruses have been implicated into the pathogenesis of IPF yet there is no clear evidence of viral etiology [12]. Viruses suggested to potentially have an etiologic relationship with IPF include EpsteinBarr virus (EBV), cytomegalovirus (CMV), influenza, hepatitis C virus, adenovirus, and human immunodeficiency virus type 1 (HIV-1) [13][14][15][16][17][18][19][20]. Hereditary factors may contribute to the risk of developing IPF but no specific genetic markers have been identified [21].…”

Section: Idiopathic Pulmonary Fibrosismentioning

confidence: 99%

Expanding the Spectrum of Particle-and Fiber-Associated Interstitial Lung Diseases

Ghio¹

2014

Tur Toraks Der

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While interstitial lung disease (ILD) is common, less than five percent of diagnoses appear to be related to occupational and environmental exposures to particles and fibers; these injuries are most frequently recognized as pneumoconioses and hypersensitivity pneumonitides. As a result of this small contribution to recognized ILD, the association of particles and fibers with this group of diseases is frequently deemed of little consequence. However, it has been proposed that some portion of ILD without a recognized cause (i.e. idiopathic disease) can also be related to exposures to particles and fibers. Many of these diagnoses are determined without benefit of microscopic examination of lung tissue. Even when tissue is available, the conventional examination for the presence of particles and fibers is used. This approach employing optical microscopy is insensitive and leads to misdiagnosis. A review of previous investigation demonstrates associations of idiopathic pulmonary fibrosis, desquamative interstitial pneumonia, pulmonary alveolar proteinosis, sarcoidosis, and eosinophilic granuloma with particle and fiber exposures. It is recommended that scanning electron microscopy in combination with the use of electron dispersive X-ray analysis be employed whenever the question of a possible relationship between ILD and particle and fiber exposure arises.

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“…A higher incidence of Epstein-Barr virus (EBV), influenza, cytomegalovirus [29], and hepatitis C [30] infections have been reported in patients with IPF. EBV in particular has been shown to be present in the lungs in patients with IPF and that they replicate in epithelial cells.…”

Section: Risk Factorsmentioning

confidence: 99%

Idiopathic Pulmonary Fibrosis

Keating¹,

McCullagh²,

Egan³

2007

Diffuse Parenchymal Lung Disease

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Idiopathic pulmonary fibrosis (IPF) is an increasingly common condition which poses many challenges. High resolution CT scanning offers a non-invasive technique which can secure an accurate diagnosis in the majority of patients. Lung function estimates of gas transfer allow the definition of advanced (DLco Ͻ 39% predicted) and limited disease (DLco Ͼ 40% predicted). Developments in the area of medical therapy have been slow. Based on current thinking regarding the pathophysiology of IPF, steroids are increasingly seen as having only a limited role in its treatment. Emerging studies are highlighting the importance of alternative forms of therapy including N-acetylcysteine, pirfenidone and bosentan, for example. Lung transplantation is a legitimate surgical treatment for IPF. However, in light of the high death rate on the waiting list, it is recommended that selected patients are referred early to the transplant programme.

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Three Sensitive Methods for the Detection of Cytomegalovirus in Lung Tissue of Patients with Interstitial Pneumonitis

Cited by 39 publications

References 18 publications

Comparison of different methods for CMV detection

Comparison of different methods for CMV detection

Expanding the Spectrum of Particle-and Fiber-Associated Interstitial Lung Diseases

Idiopathic Pulmonary Fibrosis

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