This study evaluated the influence of acute fatigue on shooting skill performance in goalball penalty shots. Eleven sub-elite male players were evaluated in an official court using a two-dimensional procedure (60 Hz) to compute the mean radial error, bivariate variable error and accuracy, and three-dimensional kinematics (240 Hz) to measure ball velocity. Rating of perceived exertion, blood lactate concentration and knee extension isometric maximal voluntary contractions were assessed at rest and immediately after the completion of an incremental test specific to goalball. Although significant differences were observed for rating of perceived exertion (pre: M = .36 AU, SD = .67; post: M = 9.27 AU, SD = .79), blood lactate concentration (M = 1.33 mM, SD = .51; M = 9.04 mM, SD = 2.25) and peak force (M = 597.85 N, SD = 132.35; M = 513.37 N, SD =107.00) at the end of the incremental test, no differences were identified in the pre and post moments for mean radial error (M = .10 m, SD = 1.01; M =.37 m, SD = 2.19), bivariate variable error (M = 3.56 m, SD = .70; M = 3.25 m, SD = .70), accuracy (M = 3.68 m, SD = .74; M = 3.78 m, SD = 1.13) and ball velocity (M = 18.53 m . s -1 , SD = 1.53; M =18.46 m . s -1 , SD = 1.65). We concluded that even though significant levels of fatigability can be attributed to the incremental test specific to goalball, shooting skill performance in goalball penalty shots does not appear to be affected. However, further investigations are needed so that goalball coaches can develop strategies that contribute to improve performance in this technical action. Such improvements are decisive for the outcome of a match.