In this study, in vitro antimicrobial and antioxidant activity of Plagiomnium medium (Bruch & Schimp.), Leptodon smithii (Hedw.), Platyhypnidium alopecuroides (Brid.), Pterogonium gracile (Hedw.), Pylasia polyantha (Hedw.) and Timmia bavarica (Hessl.) was investigated. The antimicrobial activity of prepared moss ethanol extracts was determined using the disk diffusion method against 20 strains. The DPPH (2,2-difenil-1-pikrilhidrazil) method was used to determine the antioxidant activity. As a result of the antimicrobial activity test, it was found to be effective against multi-drug resistant and standard Staphylococcus aureus strains, which is an important hospital infection. As a result of the antioxidant activity test, an antioxidant effect was observed in all samples and it was determined that the P. medium sample had the highest effect with an EC50 value of 6.0826 μg/mL.