Three types of cilia including a novel 9+4 axoneme on the notochordal plate of the rabbit embryo

Feistel, Kerstin; Blum, Martin

doi:10.1002/dvdy.20986

Cited by 87 publications

(68 citation statements)

References 60 publications

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“…3E-K). This expression pattern might correspond to that described in rabbits where foxj1 is expressed in the notochordal plate as the cells migrate from Hensen's node anteriorly, and eventually is expressed in the notochord (Feistel and Blum, 2006). However, in zebrafish, foxj1 expression was not detected in the notochord but in the floor plate as visualized by DIC optics (not shown) and by double in situ staining with ntl as a notochord marker (Fig.…”

Section: Expression Of Foxj1 During Zebrafish Embryogenesissupporting

confidence: 82%

“…In other organs, sensory cilia with a 9+0 microtubule pattern, such as those on olfactory neuroepithelial cells, were still present. Foxj1 is expressed in the ciliated cells of Hensen's node in the chick, and is required for left/right asymmetry determination (Blatt et al, 1999;Chen et al, 1998;Feistel and Blum, 2006;Maiti et al, 2000;Tamakoshi et al, 2006;Zhang et al, 2004). However analysis of the node in Foxj1 null mice revealed that, in contrast to the absence of airway cilia, node cilia were present.…”

Section: Abstract: Foxj1 Foxj12 Dorsal Forerunner Cell Kupffer'smentioning

confidence: 99%

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Isolation and expression analysis of foxj1 and foxj1.2 in zebrafish embryos

Aamar¹,

Dawid²

2008

Int. J. Dev. Biol.

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In this report, we present the isolation and identification of a zebrafish homolog of the winged helix\forkhead transcription factor Foxj1. Foxj1 was identified in other species but not in zebrafish. Foxj1 was shown in mice to be required in ciliogenesis and left-right asymmetry establishment. Here we present a spatio-temporal expression pattern of zebrafish foxj1, showing that this gene is expressed in dorsal forerunner cells, Kupffer's vesicle, the floor plate, pronephric ducts and kidney. This expression pattern is overlapping but different from that of the foxj1.2, the closest related gene in zebrafish. Foxj1 in zebrafish appears to have similar functions as those reported in other species connected to ciliogenesis and left-right asymmetry. KEY WORDS: Foxj1, Foxj1.2, dorsal forerunner cell, Kupffer's vesicle, floor plate, pronephric ductHepatocyte nuclear factor-3 (HNF-3)/forkhead homologue 4 (HFH-4)/Foxj1 is a winged helix/forkhead transcription factor. A 100-amino-acid DNA-binding motif, known as the winged helix, was first identified in mammalian hepatocyte nuclear factor-3 (HNF-3) and Drosophila Forkhead transcription factors (Avraham et al., 1995;Lai et al., 1993). Subsequently, many additional proteins containing the winged helix motif have been identified (Avraham et al., 1995). In humans (Pelletier et al., 1998) rats (Hackett et al., 1995) and mice, Foxj1 is expressed in ciliated cells of various tissues including the respiratory tract, brain, and ependyma in late development through adulthood, as well as in oviduct, testis, embryonic kidney (Blatt et al., 1999;Brody et al., 2000;Tichelaar et al., 1999a;Tichelaar et al., 1999b) and the choroid plexus (Blatt et al., 1999;Brody et al., 1997;Lim et al., 1997;Swetloff and Ferretti, 2005).Foxj1 is involved in the regulation of ciliogenesis and axonemal structural proteins. Foxj1 regulates basal body anchoring to the cytoskeleton of ciliated pulmonary epithelial cells, and is required for apical localization of ezrin and the formation of axonemal structures (Gomperts et al., 2004;Gomperts et al., 2007). Further, Foxj1 promotes RhoA-mediated apical actin enrichment required for ciliogenesis (Pan et al., 2007). In Foxj1 null mice, classic motile type cilia with a 9+2 microtubule ultrastructure were absent in epithelial cells, resulting in defective ciliogenesis in the airways. In other organs, sensory cilia with a 9+0 microtubule pattern, such as those on olfactory neuroepithelial cells, were still present. Foxj1 is expressed in the ciliated cells of Hensen's node in the Int. J. Dev. Biol. 52: 985-991 (2008)

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Section: Expression Of Foxj1 During Zebrafish Embryogenesissupporting

confidence: 82%

Section: Abstract: Foxj1 Foxj12 Dorsal Forerunner Cell Kupffer'smentioning

confidence: 99%

Isolation and expression analysis of foxj1 and foxj1.2 in zebrafish embryos

Aamar¹,

Dawid²

2008

Int. J. Dev. Biol.

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“…6,39 These cilia were originally reported to display 9+0 axonemes, 8 but nodal cilia with a 9+2 and even 9+4 axonemal structures have also been found in mouse, rabbit, 40,41 and zebrafish. 42 Thus, motile as well as nonmotile cilia with variable architecture of axonemal microtubules are present in multiple tissues and organs throughout the body where they regulate key events during development and in the adult.…”

Section: Tetralogy Of Fallot Tofmentioning

confidence: 98%

Cilia and coordination of signaling networks during heart development

et al. 2013

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“…It will be rewarding to investigate cilia in amphioxus. The prediction based on vertebrate LR cilia would be to find motile cilia that are ∼5 µm long, polarized to the posterior pole and perhaps exhibit a mix of different axoneme types, such as the 9+0, 9+2 and 9+4 configurations described in both rabbit and mouse (Caspary et al, 2007;Feistel and Blum, 2006).…”

Section: Lessons From Amphioxusmentioning

confidence: 99%

“…We have previously shown that Foxj1 mRNA is expressed in the Hensen's node of pig (Gros et al, 2009). The pig, like chick, lacks cilia at the posterior notochord, which otherwise resembles the ciliated LRO of the rabbit (Feistel and Blum, 2006). Foxj1 expression in the chick embryo has not yet been published; however, the chick Hensen's node does express the dynein heavy chain gene Dnah11 (previously known as left-right dynein, lrd) (Essner et al, 2002), which is induced by Foxj1 (Stubbs et al, 2008) and is required for the motility of LRO cilia (Supp et al, 1997).…”

Section: Lr Patterning During Vertebrate Evolutionmentioning

confidence: 99%

The evolution and conservation of left-right patterning mechanisms

et al. 2014

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Morphological asymmetry is a common feature of animal body plans, from shell coiling in snails to organ placement in humans. The signaling protein Nodal is key for determining this laterality. Many vertebrates, including humans, use cilia for breaking symmetry during embryonic development: rotating cilia produce a leftward flow of extracellular fluids that induces the asymmetric expression of Nodal. By contrast, Nodal asymmetry can be induced flow-independently in invertebrates. Here, we ask when and why flow evolved. We propose that flow was present at the base of the deuterostomes and that it is required to maintain organ asymmetry in otherwise perfectly bilaterally symmetrical vertebrates.

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Three types of cilia including a novel 9+4 axoneme on the notochordal plate of the rabbit embryo

Cited by 87 publications

References 60 publications

Isolation and expression analysis of foxj1 and foxj1.2 in zebrafish embryos

Isolation and expression analysis of foxj1 and foxj1.2 in zebrafish embryos

Cilia and coordination of signaling networks during heart development

The evolution and conservation of left-right patterning mechanisms

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