Three weeks of sprint interval training improved high-intensity cycling performance and limited ryanodine receptor modifications in recreationally active human subjects

Schlittler, Maja; Neyroud, Daria; Tanga, Christian; Zanou, Nadège; Kamandulis, Sigitas; Skurvydas, Albertas; Kayser, Bengt; Westerblad, Håkan; Place, Nicolas; Andersson, Daniel

doi:10.1007/s00421-019-04183-w

Cited by 17 publications

(27 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In line with the wellestablished role of PGC-1 coactivators in the physiological adaptation to physical exercise (Correia et al, 2015), Nrtn expression was elevated in mouse skeletal muscle after 8 weeks of voluntary wheel running (Figure 1A). Likewise, we observed a robust activation of NRTN expression in human vastus lateralis muscle after a single session of high-intensity sprint cycling (Schlittler et al, 2019) (Figure 1B). Consistent with these results, Nrtn expression in skeletal muscle correlates positively with the improvement of VO2 max with exercise and negatively with blood glucose levels in the BXD mouse genetic reference population (Figure S1B).…”

Section: Skeletal Muscle-derived Nrtn Promotes Morphological and Transcriptional Mn Remodelingsupporting

confidence: 58%

Muscle-secreted neurturin couples myofiber oxidative metabolism and slow motor neuron identity

Correia

Kelahmetoglu

Jannig

et al. 2021

Preprint

View full text Add to dashboard Cite

Endurance exercise promotes skeletal muscle vascularization, oxidative metabolism, fiber-type switching, and neuromuscular junction integrity. Importantly, the metabolic and contractile properties of the muscle fiber must be coupled to the identity of the innervating motor neuron (MN). Here, we show that muscle-derived neurturin (NRTN) acts on muscle fibers and MNs to couple their characteristics. Using a muscle-specific NRTN transgenic mouse (HSA-NRTN) and RNA-sequencing of MN somas, we observed that retrograde NRTN signaling promotes a shift towards a slow MN identity. In muscle, NRTN increased capillary density, oxidative capacity, and induced a transcriptional reprograming favoring fatty acid metabolism over glycolysis. This combination of effects on muscle and MNs, makes HSA-NRTN mice lean with remarkable exercise performance and motor coordination. Interestingly, HSA-NRTN mice largely recapitulate the phenotype of mice with muscle-specific expression of its upstream regulator PGC-1a1. This work identifies NRTN as a myokine that couples muscle oxidative capacity to slow MN identity.

show abstract

Section: Skeletal Muscle-derived Nrtn Promotes Morphological and Transcriptional Mn Remodelingsupporting

confidence: 58%

Muscle-secreted neurturin couples myofiber oxidative metabolism and slow motor neuron identity

Correia

Kelahmetoglu

Jannig

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…We have previously shown that SIT sessions can induce modifications in the RyR1 resulting in a reduction in full-length protein expression in Western blots [ 19 , 26 ]. Therefore, we assessed the full-length RyR1 expression in muscle biopsies taken before and 24 h after the first (untrained state) and last (trained state) SIT sessions ( Figure 3 .…”

Section: Resultsmentioning

confidence: 99%

“…PLFFD can, in principle, be due to decreased SR Ca 2+ release and/or reduced myofibrillar Ca 2+ sensitivity, and increased ROS production has been associated to the development of PLFFD [ 23 , 51 ]. Previous studies from our group have shown that there is no direct coupling between the decrease in full-length RyR1 expression in Western blots and PLFFD after an SIT session: (i) in recreationally active individuals, the RyR1 degradation was maximal at 24 h after exercise, while force had recovered at that time [ 19 ]; (ii) PLFFD was present in elite athletes despite no signs of RyR1 fragmentation [ 19 ]; (iii) in young recreationally active individuals, three weeks of SIT (same training protocol as in the present study) resulted in some protection against the SIT-induced RyR1 modification, whereas force recovery was not improved [ 26 ]. Accordingly, in elderly recreationally active individuals, we here show slightly improved recovery of electrically evoked torques in the trained as compared to the untrained state, whereas this was accompanied by some RyR1 protection only in the vitamin group.…”

Section: Discussionmentioning

confidence: 99%

“…Then followed 4-6 repetitions of 30-s all-out cycling bouts (Wingate tests) with 4 min of rest between bouts [ 19 ]. Sessions 1 and 7–9 were composed of 6 sprints; sessions 2 and 3 were composed of four sprints, and sessions 4–6 were composed of five sprints [ 26 ]. A cycle ergometer with continuous power recording was used to quantify the amount of work produced during each SIT session.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Vitamin C and E Treatment Blunts Sprint Interval Training–Induced Changes in Inflammatory Mediator-, Calcium-, and Mitochondria-Related Signaling in Recreationally Active Elderly Humans

et al. 2020

Self Cite

View full text Add to dashboard Cite

Sprint interval training (SIT) has emerged as a time-efficient training regimen for young individuals. Here, we studied whether SIT is effective also in elderly individuals and whether the training response was affected by treatment with the antioxidants vitamin C and E. Recreationally active elderly (mean age 65) men received either vitamin C (1 g/day) and vitamin E (235 mg/day) or placebo. Training consisted of nine SIT sessions (three sessions/week for three weeks of 4-6 repetitions of 30-s all-out cycling sprints) interposed by 4 min rest. Vastus lateralis muscle biopsies were taken before, 1 h after, and 24 h after the first and last SIT sessions. At the end of the three weeks of training, SIT-induced changes in relative mRNA expression of reactive oxygen/nitrogen species (ROS)- and mitochondria-related proteins, inflammatory mediators, and the sarcoplasmic reticulum Ca2+ channel, the ryanodine receptor 1 (RyR1), were blunted in the vitamin treated group. Western blots frequently showed a major (>50%) decrease in the full-length expression of RyR1 24 h after SIT sessions; in the trained state, vitamin treatment seemed to provide protection against this severe RyR1 modification. Power at exhaustion during an incremental cycling test was increased by ~5% at the end of the training period, whereas maximal oxygen uptake remained unchanged; vitamin treatment did not affect these measures. In conclusion, treatment with the antioxidants vitamin C and E blunts SIT-induced cellular signaling in skeletal muscle of elderly individuals, while the present training regimen was too short or too intense for the changes in signaling to be translated into a clear-cut change in physical performance.

show abstract

“…Evaluation showed that 39% of studies included familiarisations sessions for training sessions, and 53% of studies included familiarization sessions for outcome assessments. Finally, only nine studies demonstrated a priori sufficient power for their statistical analysis [44,52,57,65,66,72,80,83,88] . Additionally, only three of these studies adjusted power calculations to account for inclusion of multiple outcome variables [52,80,83] .…”

Section: Methodological Qualitymentioning

confidence: 99%

The Effects of Sprint Interval Training on Physical Performance: A Systematic Review and Meta-Analysis

Hall¹,

Aspe²,

Craig³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Sprint interval training (SIT) performed on a cycle ergometer is a common mode of training and has become increasingly studied as an exercise intervention to improve a range of physical performance outcomes. Objectives: The primary objective was to synthesise findings from published research and through meta-analysis quantify the effect of SIT and potential moderators on physical performance outcomes with healthy adults. The secondary objective was to assess the methodological quality of included studies and the existence of small study effects. Methods: The review included studies from 2000 to 2020 based on the following criteria: 1) healthy participants 18 to 45 years; 2) minimum 2-week SIT intervention comprising “all out” sprints up to a maximum of 30 seconds on a cycle ergometer; 3) cohort or control group design; 4) pre-post intervention outcomes that could be categorised as aerobic (e.g. VO2max, incremental time); anaerobic (e.g. peak power, fatigue index); mixed aerobic/anaerobic (e.g. critical power test, peak power across repeated tests); or muscular force (e.g. maximal voluntary contraction, peak force). Methodological quality was assessed using a modified Downs and Black Checklist and a strategy based on the recommendations of the Grading of Recommendations Assessment Development and Evaluation working group. All meta-analyses were conducted within a Bayesian framework to facilitate probabilistic interpretations of mean standardised effect sizes obtained from both non-controlled and non-exercise-controlled data. Results: Fifty-five studies met the inclusion criteria, with 50% graded as moderate methodological quality and 42% graded as low methodological quality. Most studies (58%) comprised an intervention of 4 weeks or less, with the collective research featuring a wide range of training protocols. The primary meta-analysis was conducted on non-controlled effect sizes from 403 outcomes. The analysis identified a small to medium effect of improved physical performance with SIT (ES0.5 = 0.43 [95%CrI: 0.35 to 0.52]). Moderator analyses identified large differences among outcome types, with no evidence of an effect for muscular force outcomes (ES0.5 = 0.05 [95%CrI: -0.25 to 0.35]) and the largest effect estimated for anaerobic outcomes (ES0.5 = 0.57 [95%CrI: 0.33 to 0.86]). Additionally, moderation effects were identified for intervention duration, sprint length, and the number sprints performed per session, with larger effects obtained for greater values of each moderator. Many positive outliers were identified with additional evidence of extensive small study effects. Conclusion: This meta-analysis demonstrates that short-term SIT interventions are effective for developing small to moderate improvements in a range of physical performance outcomes across the aerobic to anaerobic spectrum. However, extensive small study effects, likely influenced by researchers analysing many outcomes, suggests potential overestimation of reported effects. It is recommended that future research analyse a smaller number of a priori selected outcomes and investigate models to progress SIT interventions for longer-term performance improvements.

show abstract

Three weeks of sprint interval training improved high-intensity cycling performance and limited ryanodine receptor modifications in recreationally active human subjects

Cited by 17 publications

References 19 publications

Muscle-secreted neurturin couples myofiber oxidative metabolism and slow motor neuron identity

Muscle-secreted neurturin couples myofiber oxidative metabolism and slow motor neuron identity

Vitamin C and E Treatment Blunts Sprint Interval Training–Induced Changes in Inflammatory Mediator-, Calcium-, and Mitochondria-Related Signaling in Recreationally Active Elderly Humans

The Effects of Sprint Interval Training on Physical Performance: A Systematic Review and Meta-Analysis

Contact Info

Product

Resources

About