Thrips and tospoviruses come of age: Mapping determinants of insect transmission

Ullman, Diane E.; Whitfield, Anna E.; German, Thomas L.

doi:10.1073/pnas.0501341102

Cited by 24 publications

(13 citation statements)

References 26 publications

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“…SpeciÞc interactions between pathogen surface proteins and receptors in the midgut, salivary glands, or other vector tissues have been reported in other pathogenÐvector systems, e.g., rhabdoviruses and bunyaviruses (Ullman et al 2006, Ammar et al 2009), Spiroplasma and other bacteria (Fletcher et al 1998, Yu et al 2000, Bové et al 2003, Weintraub and Beanland 2006, Killiny et al 2006, Suzuki et al 2006, and Plasmodium (Ghosh et al 2001). These interactions are thought to be major determinants of the speciÞcity with which such pathogens are transmitted only by certain species, races, or developmental stages of their insect vectors (Ammar 1994, Bové et al 2003, Weintraub and Beanland 2006.…”

Section: Discussionmentioning

confidence: 96%

Detection and Relative Titer ofCandidatusLiberibacter asiaticus in the Salivary Glands and Alimentary Canal ofDiaphorina citri(Hemiptera: Psyllidae) Vector of Citrus Huanglongbing Disease

Ammar¹,

Shatters²,

Lynch

et al. 2011

Annals of the Entomological Society of America

122

119

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Candidatus Liberibacter asiaticus (CLas) bacterium has been strongly implicated as the causative agent of huanglongbing (HLB), or citrus greening, which is currently the most devastating citrus disease worldwide. HLB is transmitted by the Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae), in a persistent manner. We used quantitative-polymerase chain reaction (PCR) to detect CLas in dissected organs of individual D. citri adults infected with HLB in the laboratory or collected from field-infected citrus trees in South Florida. The proportion of infected (CLas-positive) dissected organs was 47–70% for the salivary glands, 72–80% for the alimentary canal, and 79–97.5% for the rest of the insect body. Statistical analysis indicated that, in both field- and laboratory-infected D. citri, the proportion of infected salivary glands was significantly lower than that of other parts in the insect body. With field-collected psyllids, the relative copy number of CLas genomes, compared with psyllid genomic DNA in each sample, was significantly higher in both the salivary gland and alimentary canal compared with that in the rest of the insect body for both males and females. These results provide the first PCR confirmation of CLas in the alimentary canal and salivary glands of D. citri and strongly suggest that the salivary glands constitute an important transmission barrier to CLas in the psyllid vector. Our results also suggest that CLas may replicate or accumulate in both the alimentary canal and salivary glands of D. citri.

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Section: Discussionmentioning

confidence: 96%

Detection and Relative Titer ofCandidatusLiberibacter asiaticus in the Salivary Glands and Alimentary Canal ofDiaphorina citri(Hemiptera: Psyllidae) Vector of Citrus Huanglongbing Disease

Ammar¹,

Shatters²,

Lynch

et al. 2011

Annals of the Entomological Society of America

122

119

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“…During the infection process of persistent, propagative viruses such as rhabdoviruses (family Rhabdoviridae ), tospoviruses (family Bunyaviridae ), tenuiviruses and reoviruses (family Reoviridae ), viruses encounter multiple barriers to acquisition, replication, intercellular movement, cell escape and host plant inoculation [50,82,83,84]. Virus and insect proteins thought to be involved in overcoming these barriers [3,83] are only beginning to be identified.…”

Section: Critical Steps In Virus–insect Interactions As Potential mentioning

confidence: 99%

“…Virus and insect proteins thought to be involved in overcoming these barriers [3,83] are only beginning to be identified. Rhabdovirus and tospovirus surface glycoproteins are required for virus entry into vector cells (genetic determinants of transmissibility), but their counterpart receptors in the insect gut have yet to be identified [62,82,84]. Identification of receptor-like determinants in the insect vector that viruses bind to would constitute a major breakthrough since they could become novel targets to control virus acquisition.…”

Section: Critical Steps In Virus–insect Interactions As Potential mentioning

confidence: 99%

Plant Virus–Insect Vector Interactions: Current and Potential Future Research Directions

Dietzgen

Mann

Johnson

2016

Viruses

196

142

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Acquisition and transmission by an insect vector is central to the infection cycle of the majority of plant pathogenic viruses. Plant viruses can interact with their insect host in a variety of ways including both non-persistent and circulative transmission; in some cases, the latter involves virus replication in cells of the insect host. Replicating viruses can also elicit both innate and specific defense responses in the insect host. A consistent feature is that the interaction of the virus with its insect host/vector requires specific molecular interactions between virus and host, commonly via proteins. Understanding the interactions between plant viruses and their insect host can underpin approaches to protect plants from infection by interfering with virus uptake and transmission. Here, we provide a perspective focused on identifying novel approaches and research directions to facilitate control of plant viruses by better understanding and targeting virus–insect molecular interactions. We also draw parallels with molecular interactions in insect vectors of animal viruses, and consider technical advances for their control that may be more broadly applicable to plant virus vectors.

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“…Additional information on the mechanics of plant virus transmission by insects is reviewed in Ammar and Nault (2002), Brown and Czosnek (2002), Gray and Gildow (2003), Hogenhout et al (2003), Ng and Perry (2004), and Ullman et al (2005). This can occur because of selection for mutations or deletions in genes for critical components of the virus required for vector transmission, but dispensable for host plant infection.…”

Section: Critical Parametersmentioning

confidence: 99%

Aphid Transmission of Plant Viruses

Gray

2008

CP Microbiology

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A majority of plant viruses are transmitted between hosts by insect vectors, and it is often important to use insect transmission in the laboratory to maintain virus isolates or to study virus‐vector‐plant interactions. Although many of these viruses can also be mechanically transmitted in the laboratory using infected sap, maintenance by mechanical transmission can often lead to changes in the virus, either minor changes in gene sequences or, in some cases, major deletions of genome sequences. These can affect both virus‐vector and virus‐host interactions. This unit describes some simple and practical methods for conducting virus transmission experiments using sap‐sucking insects. Curr. Protoc. Microbiol. 10:16B.1.1‐16B.1.10. © 2008 by John Wiley & Sons, Inc.

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Thrips and tospoviruses come of age: Mapping determinants of insect transmission

Cited by 24 publications

References 26 publications

Detection and Relative Titer ofCandidatusLiberibacter asiaticus in the Salivary Glands and Alimentary Canal ofDiaphorina citri(Hemiptera: Psyllidae) Vector of Citrus Huanglongbing Disease

Detection and Relative Titer ofCandidatusLiberibacter asiaticus in the Salivary Glands and Alimentary Canal ofDiaphorina citri(Hemiptera: Psyllidae) Vector of Citrus Huanglongbing Disease

Plant Virus–Insect Vector Interactions: Current and Potential Future Research Directions

Aphid Transmission of Plant Viruses

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