Thrombin detection using a piezoelectric aptamer-linked immunosorbent assay

Collins, Cheryl; Yui, S.; Roberts, Charles; Kojic, Igor

doi:10.1016/j.ab.2013.08.019

Cited by 13 publications

(5 citation statements)

References 48 publications

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“…Due to this, the assay would have been implemented only if the drug's efficacious dose would have been found to be lower than actual. Other published data also suggests that the narrow range might be a limitation of the technology (6,7).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Acoustic Membrane Microparticle (AMMP) Technology for a Sensitive Ligand Binding Assay to Support Pharmacokinetic Determinations of a Biotherapeutic

Chilewski

Dickerson

Mora

et al. 2014

AAPS J

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Abstract. Achieving the required sensitivity can be a challenge in the development of ligand binding assays for pharmacokinetic (PK) determinations of biotherapeutics. To address this need, BioScale's Acoustic Membrane Microparticle (AMMP) technology was evaluated for the quantification of a PEGylated domain antibody (dAb) biotherapeutic. Previous uses of this technology had shown utility in biomarker and process development applications and this is the first application, to our knowledge, for PK determinations. In this evaluation, AMMP was capable of delivering a sensitivity of 0.750 ng/mL, which surpasses the sensitivity requirements for the majority of assays to support PK determinations. This evaluation demonstrates that this emerging technology has the ability to produce the required sensitivity, reproducibility, and selectivity needed to meet the industry's standards for PK analysis.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Acoustic Membrane Microparticle (AMMP) Technology for a Sensitive Ligand Binding Assay to Support Pharmacokinetic Determinations of a Biotherapeutic

Chilewski

Dickerson

Mora

et al. 2014

AAPS J

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“…While contemplating the answer to such questions, inspection of one of the most prolific aptamers, the thrombin-binding aptamer, may provide insight. The proof-of-concept analytical applications of thrombin aptamers have been demonstrated through various examples including designing thrombin biosensors involving various transducers, − using duplexed aptamers based on thrombin aptamers for exploring the ligand binding dynamics, ,, improving signal-to-background ratio and sensitivity of the thrombin aptamer by using split thrombin aptamer fragments, − programming multiprotein nanoarrays with defined nanometer spacing and precision employing thrombin aptamers, constructing target-specific imaging platforms based on thrombin aptamers for in vivo imaging, − etc. Given these across-the-board applications, we believe the secret of the success of the thrombin aptamer is 2-fold: (1) thrombin aptamers are proven to work via a multidisciplinary array of methods, (2) the binding domain of thrombin aptamers and intermolecular forces between thrombin aptamers and the target are well characterized and the specificity is well documented.…”

Section: Analytical Application Of Aptamers: Highlight the Advantages...mentioning

confidence: 99%

Perspective on the Future Role of Aptamers in Analytical Chemistry

et al. 2019

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It has been almost 30 years since the invention of Systematic Evolution of Ligands by Exponential Enrichment (SELEX) methodology and the description of the first aptamers. In retrospect over the past 30 years, advances in aptamer development and application have demonstrated that aptamers are potentially useful reagents that can be employed in diverse areas within analytical chemistry, biotechnology, biomedicine, and molecular biology. While often touted as artificial antibodies with an ability to be selected for any target, aptamer development, unfortunately, lags behind development of analytical methodologies that employ aptamers, hindering deeper integration into the application of analytical tool development. This perspective covers recent advances in SELEX methodology for improving efficiency of the SELEX procedure and enhancing affinity and specificity of the selected aptamers, what we view as a critical barrier in the future role of aptamers in analytical chemistry. We discuss postselection modifications that can be used for enhancing performance of the selected aptamers in an analytical device by including understanding intermolecular interaction forces in the binding domain. While highlighting promising properties of aptamers that enable several analytical advances, we provide discussion on the challenges of penetration of aptamers in the analytical field.

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“…5,6 Moreover, recent studies have made progress in thrombin detection, including enzyme-linked sandwich assays, 7,8 strips, 9−11 optical sensors, 12−14 electrochemical platforms, 15−17 and piezoelectric methods. 18 In general, enzyme-linked sandwich assays are the gold standard for thrombin detection 11 but also experience laborious steps, low analysis speed, and false positives. Lateral flow assays (strips) are rapid, cost effective, and great for point-of-care (POC) tests but usually have higher detection limits or qualitative results without additional professional equipment.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Thrombin is a serine protease, an enzyme that plays a key role in blood coagulation, and is relevant to a plethora of diseases such as chronic inflammatory diseases, vascular disease, coagulation abnormalities, cancer, and atherosclerosis. − Generally, excessive thrombin will cause venous thrombosis, and a low amount of thrombin will increase bleeding risks . The concentration of thrombin during coagulation varies from picomolar to micromolar levels, and thus, the limit of detection (LOD) and detection range need to cover the picomolar to micromolar range. , Moreover, recent studies have made progress in thrombin detection, including enzyme-linked sandwich assays, , strips, − optical sensors, − electrochemical platforms, − and piezoelectric methods . In general, enzyme-linked sandwich assays are the gold standard for thrombin detection but also experience laborious steps, low analysis speed, and false positives.…”

Section: Introductionmentioning

confidence: 99%

Label-Free Quartz Crystal Microbalance Biosensor Based on Aptamer-Capped Gold Nanocages Loaded with Polyamidoamine for Thrombin Detection

Niyonshuti

et al. 2021

ACS Appl. Nano Mater.

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Thrombin is an important biomarker, and its detection is of great significance for diagnosis and prevention of related diseases. Conventional methods such as enzyme-linked immunosorbent assay are sensitive but require multiple steps. In this work, a label-free biosensor that only required one step of incubation was developed based on target-triggered release of the cargo molecules from gold nanocages, which achieved highly sensitive and specific detection of thrombin. The proposed biosensor consists of an array of gold nanocages loaded with molecules in their interiors and the DNA probes immobilized on their surface for hybridization with thrombin-specific aptamers to seal their pores. Upon interaction with thrombin, the surface aptamers were lifted off the gold nanocages, resulting in release of the cargo molecules. The loss of cargo molecules was detected by quartz crystal microbalance (QCM). The signal could be amplified by the choice of cargo molecules. The use of polyamidoamine as cargo molecules allowed us to achieve a label-free biosensor with a linear detection range of 0.0086−86 nM and a limit of detection of 7.7 pM. The biosensor was also tested with spiked human serum samples with a limit of detection of 1.2 nM. The detection could be carried out within 1.5 h with only one incubation step of 45 min. The specificity of the biosensor was confirmed by testing against bovine serum albumin and lysozyme at 1 μM.

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Thrombin detection using a piezoelectric aptamer-linked immunosorbent assay

Cited by 13 publications

References 48 publications

Evaluation of Acoustic Membrane Microparticle (AMMP) Technology for a Sensitive Ligand Binding Assay to Support Pharmacokinetic Determinations of a Biotherapeutic

Evaluation of Acoustic Membrane Microparticle (AMMP) Technology for a Sensitive Ligand Binding Assay to Support Pharmacokinetic Determinations of a Biotherapeutic

Perspective on the Future Role of Aptamers in Analytical Chemistry

Label-Free Quartz Crystal Microbalance Biosensor Based on Aptamer-Capped Gold Nanocages Loaded with Polyamidoamine for Thrombin Detection

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