Thromboelastography in healthy horses and horses with inflammatory gastrointestinal disorders and suspected coagulopathies

Méndez-Angulo, José L.; Mudge, Margaret C.; Saavedra, Paulo Vilar; Stingle, Nicole; Couto, C. Guillermo

doi:10.1111/j.1476-4431.2010.00576.x

Cited by 34 publications

(47 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the method is not as common in veterinary medicine as in human medicine even though the test is not species-dependent. In recent years, the number of studies on equine thromboelastography has been slowly rising (e.g., Epstein et al 2009;Mendez-Angulo et al 2010;Epstein et al 2011;Hyldahl-Laursen et al 2013;Scruggs et al 2016), but the physiological values required for the clinical use of this method are still unknown. Further testing, including comparison of thromboelastography and standard coagulation testing, is required.…”

mentioning

confidence: 99%

Evaluation of kaolin-activated thromboelastography and sample stability in healthy horses

Machackova¹,

Boselova²,

Vanova³

et al. 2018

Vet. Med. - Czech

View full text Add to dashboard Cite

Thromboelastography is an accurate alternative to routine coagulation testing for the monitoring of haemostasis. However, its use in equine medicine is limited not only by the lack of reference interval values for kaolin-activated citrated samples, but also by the limited accessibility of the test for field practitioners within the 2-hour storage time recommended by the manufacturer. To address this issue, we here evaluated kaolin-activated thromboelastography using a TEG<sup>®</sup> 5000 Thrombelastograph<sup>®</sup> Hemostasis System in 36 healthy horses, and sample stability was evaluated at four timepoints post collection in seven horses. Reference values were established as follows: reaction-time 5.0–16.0 min, K-time (period in which the clot strength reaches 20 mm of amplitude) 1.1–5.2 min, α-angle (speed of fibrin cross-linking) 36.5–79.0°, maximal amplitude 44.5–69.7 mm, fibrinolysis 30 minutes after maximal amplitude was reached 0.0–2.8%. During storage, the trends in the changes of values were similar for most parameters, and values remained mostly within the reference intervals. Thromboelastography is thus useful in defining thrombohaemorrhagic complications in horses but can be sensitive to preanalytical factors and storage.

show abstract

mentioning

confidence: 99%

Evaluation of kaolin-activated thromboelastography and sample stability in healthy horses

Machackova¹,

Boselova²,

Vanova³

et al. 2018

Vet. Med. - Czech

View full text Add to dashboard Cite

show abstract

“…Most previous studies on haemostatic changes in the course of colic were mainly focused on secondary haemostasis (Dallap et al 2003;Dallap Schaer and Epstein 2009). Only a few studies assessed platelet function in horses with gastrointestinal disease using aggregometry or thromboelastography (Epstein et al 2011;Brooks et al 2007;Mendez-Angulo et al 2010;Epstein et al 2013); however, to our knowledge, there have been no reports evaluating platelet function in horses with colic using the PFA-100. Considering the potential usefulness of the PFA-100 in the assessment of equine platelet function, it was decided to investigate platelet function in the course of acute surgical colic.…”

mentioning

confidence: 76%

Evaluation of platelet function in horses undergoing colic surgery using the PFA-100 platelet function analyser

Iwaszko-Simonik¹,

Graczyk²

2015

Vet. Med. - Czech

View full text Add to dashboard Cite

Acute colic in horses, especially presentations requiring surgical correction, such as large colon volvulus, very often involves defective or excess platelet activation. The PFA-100 is a new point-of-care analyser that evaluates platelet function by measuring closure time (CT) in the whole blood of healthy horses in a standardised manner. However, there are no reports on platelet function in horses with colic measured by the PFA-100. The aim of the present study was to investigate platelet function in a group of horses suffering ascending colon displacement (ACD) and who underwent surgery compared to a group of control (C) horses. Thirty ACD cases and twenty untreated clinically healthy control horses were included. Blood samples were collected from the ACD horses prior to and 24, 48 and 72 h after laparotomy. Red blood cell count (RBC), haematocrit (HCT), haemoglobin (Hb) concentration, white blood cell count (WBC), total plasma protein (TP) concentration, platelet count (PLT), platelet volume (MPV) and ADP closure time (CT-ADP) were measured. CT was determined on a PFA-100® device using collagen/ADP cartridges. There were no significant differences in RBC, PLT and MPV between C and ACD horses pre-operatively. Reduced PLT was observed in the post-operative period. The mean CT-ADP of C horses was 96.43 ± 12.53 and 91.43 ± 2.51 seconds in the ACD group prior to surgery. CT-ADP was changed after surgery whereby CT-ADP time was more than doubled. We conclude that the PFA-100 ® can be used to detect platelet function defects in horses.

show abstract

“…Through thromboelastometry, we can evaluate the effect of heparin and control its therapy by graphic indicators of the interval of clotting time and clot formation, which is more judicious managed with the availability of specific reagents, such as Heptem ® -a heparinase that is inactive, thus facilitating the identification of anomalous effects from the therapy and the immediate correction of the dose or suspension of medication (Donahue & Otto 2005, Epstein et al 2009, Kol & Borjesson 2010, Mendez--Angulo et al 2010.…”

Section: Discussionmentioning

confidence: 99%

“…TEG ® and ROTEM ® , both apparatuses of thromboelastography, furnish a graphic representation of the formation and subsequent lysis of the coagulum, but differ in their system of detection and collection of data, utilizing a torsion wire and optical detector, respectively (Reikvam et al 2009, Kol & Borjesson 2010, Mendez-Angulo et al 2010, Wiinberg & Kristensen 2010. The interest in their use in equine medicine is focused primarily on distinguishing states of hypo-and hypercoagulability, in addition to evaluating coagulation and fibrinolysis (Dias & De Lacerda Neto 2013).…”

Section: Introductionmentioning

confidence: 99%

“…These disturbances are frequently monitored by means of a coagulogram -exams that analyze specific factors in the plasma (platelet count, fibrinogen, bleeding time, platelet function, activated clot time, thrombin time [TT], prothrombin time [PT] and activated partial thromboplastin time [aPTT]), responsible for the clotting system (Reikvam et al 2009, Mendez--Angulo et al 2010. These routine tests provide fragmented pieces of information on the clotting process while, for the diagnosis of coagulopathies, many of them need to be performed jointly (Kol & Borjesson 2010).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Thromboelastometric evaluation of horses submitted to experimental thrombosis and jugular thrombectomy

et al. 2016

View full text Add to dashboard Cite

Jugular thrombosis in horses occurs commonly in iatrogenic situations, secondary to endotoxemic clinical condition and disseminated vascular coagulation, potentially leading to death. Thus, hemostatic evaluation becomes necessary and extremely important for monitoring the risks of systemic hypercoagulability and for the efficiency of allopathic and surgical treatment. This paper describes the hemostatic behavior in experimental jugular thrombosis of ten healthy equines, subsequently submitted to two thrombectomy techniques and receiving heparin sodium as anti-rethrombosis therapy. These animals were evaluated for 20 days by thromboelastometry (TEM), platelet count, hematocrit and fibrinogen, at four moments: pre-induction to phlebitis (D0-MPF); three days after thrombophlebitis induction (D3-MFM); 6 days after, -moment of thrombophlebitis -(D9-MT); and 54 (D16) and 126 (D19) hours after thrombectomies (PTM). Thrombectomy was performed via a Vollmar Ring (group 1, n=5) and Fogarty catheter (group 2, n=5). All the animals received heparin (150 UI/kg, SC) every 12 hours, for ten days after the respective thrombectomies. Through the blood samples were evaluated TEM, activated partial thromboplastin time (aPTT) and prothrombin time (PT), dosing of fibrinogen, hematocrit and platelet count at the abovementioned moments. For comparison between groups and moments the t test was applied at 5% significance level. No significant difference was verified between treatment groups at any of the moments. There were reductions in clotting time (CT) and clot formation time (CFT), with increase in maximum lysis (ML) until the moment D9-MT. Evaluation through INTEM ® reagent presented prolongations of CT and CFT with reduction of α angle and ML starting from D16 and D19. Similarly, aPTT presented significant differences between moments pre-(D0, 3 and 9) and post-(D16 and 19) anticoagulant and surgical treatment. The platelet numbers were diminished at moments D16 and D19. In evaluation with EXTEM ® reagent, prolongation of CT and CFT occurred only between the moments D0 vs. D3 and vs. D9. O PT did not present significant differences. The results obtained demonstrate that experimental jugular thrombophlebitis leads to local clinical alterations, with impairment of tissue and of the extrinsic coagulation pathway (EXTEM ® ), but without evidence of systemic hypercoagulability status, since there was no increase of the alpha angle or maximum clot firmness (MCF). Furthermore, TEM was shown useful and more sensitive than conventional coagulation tests (PT, aPTT and fibrinogen) for the monitoring of anticoagulant therapy, as demonstrated in other works. Houve diminuição do número de plaquetas nos momentos D16 e D19. Na avaliação com reagente extem ocorreu apenas o prolongamento do CT e CFT entre os momentos D0 e o D3 e D9. O TP não apresentou diferenças significativas. Os resultados obtidos demonstram que a tromboflebite jugular experimental leva a alterações clínicas locais, com comprometimento tecidual e da via extrínseca da coagulação ...

show abstract

Thromboelastography in healthy horses and horses with inflammatory gastrointestinal disorders and suspected coagulopathies

Cited by 34 publications

References 22 publications

Evaluation of kaolin-activated thromboelastography and sample stability in healthy horses

Evaluation of kaolin-activated thromboelastography and sample stability in healthy horses

Evaluation of platelet function in horses undergoing colic surgery using the PFA-100 platelet function analyser

Thromboelastometric evaluation of horses submitted to experimental thrombosis and jugular thrombectomy

Contact Info

Product

Resources

About