Thrombozytopenie beim experimentellen HIPA Plasmozytom der BALB/c Maus

Schweyer, H.; Rüttner, J. R.; Pedio, G

doi:10.1159/000208696

Cited by 3 publications

(4 citation statements)

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“…Origin and features of HIPA tumor were reported in previous publications (Pedio et al, 1968a;Grieshaber et al, 1969a, b;Pedio et al, PEDIO AND RUTTNER 1969;Busser et al, 1970;Schweyer et al, 1970;Pedio and Riittner 1971a;Pedio and Riittner 1971b. ) For the present experiments HIPA tumor ascites from the 16th to 22th cell-free passages, line UZ/G, was used.…”

Section: Hipa Plasmacytomasupporting

confidence: 59%

Inhibition of hipa plasmacytoma cell‐free transmission by normal spleen of BALB/c, DBA/2 and C3H mice

Pedio¹,

Rüttner²

1971

Intl Journal of Cancer

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Spleen from normal BALB/c, DBA/2 and C3H mice exerted a protective effect against syngeneic and allogeneic cell‐free transmission of HIPA plasmacytoma in mice. The maximal protective effect of these spleens was found in C3H and ICR mice (100‐70%). Inoculation of BALB/c spleen homogenates in syngeneic mice resulted in protection of about 56% of the cases. Spleen homogenates from BALB/c mice were not effective against cellular transplantation of HIPA plasmacytoma. The fact that the protection required one week to develop suggested that an immune reaction was involved. The factor responsible for protection was found in the sedimentable fraction of spleen homogenates centrifuged at 500 × g during 15 minutes and was destroyed by heat and freezing/thawing.

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Section: Hipa Plasmacytomasupporting

confidence: 59%

Inhibition of hipa plasmacytoma cell‐free transmission by normal spleen of BALB/c, DBA/2 and C3H mice

Pedio¹,

Rüttner²

1971

Intl Journal of Cancer

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“…However, clot formation is slower if plate lets from normal mice are mixed with HIPA ascites. The reason for this difference is not known; it seems, however, not related to the number of circulating platelets, since HIPA tumor-bearing mice are usually throm bocytopenic [12].…”

Section: Discussionmentioning

confidence: 98%

“…The tumor used is an induced plasmacytoma of mice. Its morphological and biological beatures have been previously reported [7][8][9][10]12]. Briefly, the ascitic form of HIPA plasmacytoma is obtained by intraperitoneal in oculation of cell-free extracts from HIPA tumor in syngenic or allogenic mice, or by intraperitoneal inoculation of HIPA tumor cells in syngenic mice [9].…”

Section: Methodsmentioning

confidence: 99%

Interaction of Platelets with Ascitic Plasmacytoma <i>in vitro</i>

Pedio¹,

Rüttner²,

Odermatt³

1973

Acta Haematol

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During interaction in vitro of syngenic platelets from healthy mice with HIPA ascitic plasmacytoma, clot formation was slower than during interaction of syngenic platelets from HIPA tumor-bearing mice with HIPA plasmacytoma. The platelet aggregates were composed, in both cases, of ‘balloon platelets’ and tumor cells. The granulated part of the ‘balloon platelets’ made up the centre, whereas granule-free spheroids were seen at the periphery of the aggregates. To contact with platelets, the tumor cells reacted with formation of microvilli, condensation of mitochondria and phagocytosis of the granule-free spheroids. No formation of pseudopodia by platelets in contact with the tumor cells was observed.

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“…Nur C ohn [8] Thrombozytopenie beim HIPA-Plasmozytom Die intraperitoneale Inokulation von 1 ml HIPA-Aszites in isologe BALB/c-Mäuse führt in etwa 8 0 % der Fälle zu einer Senkung der Thrombozyten von durchschnittlich 975 OOO/mm5 (gesunde BALB/Maus) auf etwa 600 OOO/mm3 [41]. Diese Thrombozytopenie ist statistisch signi fikant (p > 0,01).…”

Section: Hip a -T Umoreigenschaften Verhalten Des Serumeiweisses Im Vunclassified

Das experimentelle HIPA-Plasmozytom der Maus

Pedio¹

1974

Pathobiology

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HIPA tumor is the denomination of an experimental plasmacytoma of mice. It can be transmitted by inoculation of cell-free extracts in syngenic or allogenic mice or by tumor cell transplant in syngenic mice. HIPA tumor was originally induced in the mesentery of BALB/c mice by intraperitoneal injection of spleen homogenate, obtained from a syngenic mouse, pretreated with incomplete Freund’s adjuvant. The original HIPA tumor was histologically an undifferentiated sarcoma. It underwent differentiation to plasmacytoma in the course of several transplant generations. This differentiation was characterized by loss of collagen fibers and appearance of annulate lamellae and dilatation of ergastoplasmic cysternae. HIPA tumor presented the following characteristics: (a) secretion of abnormal proteins in the course of the 3rd to 28th transplant generation; (b) appearance of a thrombocytopenía in the host after intraperitoneal inoculation of tumor cells; (c) formation of osteolytical and osteoplastic bone lesions after inoculation of tumor cells; (d) absence of splenic metastases regardless of the route of tumor inoculation. Oncogenic virus-like type C and A particles were found in the spleen of mineral oil-treated BALB/c mice and in HIPA tumor tissues of numerous transplant generations. After repeated attempts to isolate and enrich HIPA oncogenic virus by conventional methods, a double inoculation method was devised. It consisted of an initial intraperitoneal transplantation of HIPA tumor cells followed by intraperitoneal injection of HIPA cell-free tumor extracts. This procedure was repeated in several subsequent generations. Cell-free extracts of tumors which developed after double inoculation contained numerous virus-like type C particles. Such extracts induced plasmacytomas in syngenic and allogenic mice.

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Thrombozytopenie beim experimentellen HIPA Plasmozytom der BALB/c Maus

Cited by 3 publications

References 1 publication

Inhibition of hipa plasmacytoma cell‐free transmission by normal spleen of BALB/c, DBA/2 and C3H mice

Inhibition of hipa plasmacytoma cell‐free transmission by normal spleen of BALB/c, DBA/2 and C3H mice

Interaction of Platelets with Ascitic Plasmacytoma <i>in vitro</i>

Das experimentelle HIPA-Plasmozytom der Maus

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