THU0112 Anaemia in rheumatoid arthritis

Abstract

degradation. In addition, we provide for the first time experimental evidence that the acid secreted upon metabolic activation is not only a product of anaerobe (lactate, acetate) or aerobe (carbonate) glycolysis, but may be associated with a specific V-ATPase located in vacuolar membranes in the cytoplasm or on the surface of the cells. Preliminary data corroborate this conclusion, since a V-ATPase was located on the cell surface by immunohistochemistry and a V-ATPase encoding mRNA was detected by RT/PCR in the respective cells. REFERENCES 1 Parak, et al. Metabolic activation stimulates acid production in synovial fibroblasts. J Rheumatol. 2000;27:2312 2 Sainsbury, et al. Cathepsin K expression by activated fibroblasts at the bone interface of the pseudosynovium in aseptic prosthesis loosening. Proc. ORS Conv. Anaheim # 27, 1999 3 Mast A. Charakterisierung von Knochenzellen mit biophysikalischen Methoden auf zellulärem Niveau, Master Thesis,