2001
DOI: 10.1046/j.1472-765x.2001.00898.x
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Thuricin 7: a novel bacteriocin produced by Bacillus thuringiensis BMG1.7, a new strain isolated from soil

Abstract: Aims: Detection and identification of new antagonistic activities towards Bacillus cereus and relatives. Methods and Results: Twenty Bacillus thuringiensis strains were screened for their capacity to express bacteriocin‐like agents. Strain BMG1.7, isolated from soil, showed an antagonistic activity called thuricin 7. Thuricin 7 was active against several species of the genus Bacillus, including three of the four known B. thuringiensis/B. cereus bacteriocin producers, as well as against Streptococcus pyogenes … Show more

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Cited by 130 publications
(125 citation statements)
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“…lactis that is used commercially in food preservation, has a broad spectrum of activity against other lactic acid bacteria (LAB) and most Gram-positive organisms (8). Although bacteriocins produced by LAB are the most widely investigated (9, 10), bacteriocins are also produced by most other Gram-positive (11)(12)(13)(14)(15) and Gram-negative (16,17) bacterial species.…”
mentioning
confidence: 99%
“…lactis that is used commercially in food preservation, has a broad spectrum of activity against other lactic acid bacteria (LAB) and most Gram-positive organisms (8). Although bacteriocins produced by LAB are the most widely investigated (9, 10), bacteriocins are also produced by most other Gram-positive (11)(12)(13)(14)(15) and Gram-negative (16,17) bacterial species.…”
mentioning
confidence: 99%
“…The first part, consisting of samples of purified antibacterial substance and protein standards, was stained with Coomassie Brilliant blue R-250. The other part of the gel was assayed for direct detection of antibactertial substance activity according to Cherif method (Cherif et al, 2001).…”
Section: Identification Of Antibacterial Protein By Native Gel Electrmentioning
confidence: 99%
“…The gel was Coomassie Blue R-250 stained. The gel-overlay test was performed as previously described (Cherif et al, 2001): the gel was soaked in a fixing solution (25% isopropanol, 10% acetic acid) for 30 minutes, then washed twice with sterile double-distilled water for 1 hour and overlaid on a Petri dish containing 5 ml of L. monocytogenes-seeded TSA medium (4%). The Petri dish was incubated at 37°C for 24 hours and examined for the presence of a growth inhibition zone.…”
Section: Sds-page and Gel Overlay Assaymentioning
confidence: 99%