Thy-1-positive cells in the subodontoblastic layer possess high potential to differentiate into hard tissue-forming cells

Hosoya, Akihiro; Hiraga, Toru; Ninomiya, Tadashi; Yukita, Akira; Yoshiba, Kunihiko; Yoshiba, Nagako; Takahashi, Masafumi; Ito, Susumu; Nakamura, Hiroaki

doi:10.1007/s00418-012-0928-1

Cited by 42 publications

(36 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, periosteal cells sorted based on Thy1 expression showed higher proliferative capacity and osteogenic potential in vitro and in vivo compared with unsorted periosteum‐derived cell populations (30). Similarly, subodontoblastic dental pulp stem cells expressing higher Thy1 levels on the cell surface showed accelerated induction of alkaline phosphatase activity and mineralized matrix formation and a higher capacity to form hard tissue compared to the cells with lower expression of Thy1 (31). Taken together, these findings coupled with the reports outlined above, establish Thy1 as a positive regulator of OB differentiation that is centrally involved in bone homeostasis, especially in the context of obesity.…”

Section: Discussionmentioning

confidence: 99%

Thy1 is a positive regulator of osteoblast differentiation and modulates bone homeostasis in obese mice

et al. 2018

View full text Add to dashboard Cite

Thy1 (CD90), a glycosylated, glycophosphatidylinositol-anchored membrane protein highly expressed by subsets of mesenchymal stem cells and fibroblasts, inhibits adipogenesis. The role of Thy1 on bone structure and function has been poorly studied and represents a major knowledge gap. Therefore, we analyzed the long bones of wild-type (WT) and Thy1 knockout (KO) mice with micro-computed tomography (micro-CT) and histomorphometry to compare changes in bone architecture and overall bone structure. micro-CT analysis of long bones revealed Thy1 KO and WT mice fed a high-fat diet demonstrated bone structural parameters at 4 mo that differed significantly between WT and KO mice. A significant reduction in trabecular bone volume was noted in Thy1 KO mice. The most prominent differences were observed in trabecular bone volume ratio and trabecular bone connectivity density. Consistent with micro-CT measurements, histomorphometric analysis also showed decreased bone volume in the obese Thy1 KO mice compared to obese WT mice. In vitro assays revealed that osteogenic conditions increased Thy1 expression during OB differentiation and absence of Thy1 attenuated osteoblastogenesis. Together, these findings support the concept that Thy1 serves as a major mechanistic link to regulate bone formation and negatively regulate adipogenesis.-Paine, A., Woeller, C. F., Zhang, H., Garcia-Hernandez, M. L., Huertas, N., Xing, L., Phipps, R. P., Ritchlin, C. T. Thy1 is a positive regulator of osteoblast differentiation and modulates bone homeostasis in obese mice.

show abstract

Section: Discussionmentioning

confidence: 99%

Thy1 is a positive regulator of osteoblast differentiation and modulates bone homeostasis in obese mice

et al. 2018

View full text Add to dashboard Cite

show abstract

“…One day after preparation, the immunoreactivity of CD90 disappeared just under the cavity area (b). Previous studies demonstrated that CD90-highly immunoreactive cells had the ability to differentiate into odontoblasts (18,27). After 5 days, CD90-immunoreactive cells localised at the subodontoblastic layer again (d).…”

Section: Differentiation After Cavity Preparation and Its Relationshmentioning

confidence: 93%

“…In the control group, CD90-immunoreactive cells were mainly observed at the subodontoblastic layer of coronal pulp (a). stem cell marker in normal dental pulp (18,27). After 3 days, CD90immunoreactive cells were not present, whereas the rearrangement of cells indicating odontoblasts was detected in contact with the dentine (c).…”

Section: Differentiation After Cavity Preparation and Its Relationshmentioning

confidence: 99%

Changes of CD90 expression and immunoreactive cell localisation in rat dental pulp after cavity preparation

et al. 2018

View full text Add to dashboard Cite

CD90 expression and immunoreactive cell localisation in rat dental pulp cells after cavity preparation was investigated. Cavity preparation was performed on the maxillary first molar of 8-week-old Wistar rats (n = 36), and immunohistochemistry and quantitative real-time PCR were performed. CD90-immunoreactivity was observed among subodontoblastic cells in the control group. One day after cavity preparation, the CD90-immunoreactivity disappeared under the cavity area. While CD90-immunoreactivity was faint after 3 days, the re-arrangement of odontoblasts was detected in contact with dentine. After 5 days, the odontoblasts were observed beneath the dentine, and CD90-immunoreactive cells were localised under the odontoblast layer. Immunofluorescence showed co-localisation of CD90 and nestin was detected after 3 days. After 5 days, CD90-immunoreactivity increased at the subodontoblastic layer. mRNA expression of CD90 and DSPP decreased after cavity preparation, and gradually recovered (P < 0.01). These results suggest that CD90-immunoreactive cells in the subodontoblastic layer contribute to regeneration of odontoblast and subodontoblastic layers following cavity preparation.

show abstract

“…These stem cells are found at the periphery of blood vessels in the central region of the pulp and have multilineage potential (Iohara et al 2008;Karaoz et al 2011). In fact, earlier we showed that cells in the subodontoblast cell-rich layer have high mineralization ability and form a bone-like matrix after subcutaneous transplantation (Hosoya et al 2012). In addition to these possibilities, the periodontal ligament (PDL) is also thought to be a source of osteoblast-like cells.…”

mentioning

confidence: 99%

Two Distinct Processes of Bone-like Tissue Formation by Dental Pulp Cells after Tooth Transplantation

Hosoya

Yukita

Yoshiba

et al. 2012

J Histochem Cytochem.

Self Cite

View full text Add to dashboard Cite

Dental pulp is involved in the formation of bone-like tissue in response to external stimuli. However, the origin of osteoblast-like cells constructing this tissue and the mechanism of their induction remain unknown. We therefore evaluated pulp mineralization induced by transplantation of a green fluorescent protein (GFP)–labeled tooth into a GFP-negative hypodermis of host rats. Five days after the transplantation, the upper pulp cavity became necrotic; however, cell-rich hard tissue was observed adjacent to dentin at the root apex. At 10 days, woven bone-like tissue was formed apart from the dentin in the upper pulp. After 20 days, these hard tissues expanded and became histologically similar to bone. GFP immunoreactivity was detected in the hard tissue-forming cells within the root apex as well as in the upper pulp. Furthermore, immunohistochemical observation of α–smooth muscle actin, a marker for undifferentiated cells, showed a positive reaction in cells surrounding this bone-like tissue within the upper pulp but not in those within the root apex. Immunoreactivities of Smad4, Runx2, and Osterix were detected in the hard tissue-forming cells within both areas. These results collectively suggest that the dental pulp contains various types of osteoblast progenitors and that these cells might thus induce bone-like tissue in severely injured pulp.

show abstract

Thy-1-positive cells in the subodontoblastic layer possess high potential to differentiate into hard tissue-forming cells

Cited by 42 publications

References 45 publications

Thy1 is a positive regulator of osteoblast differentiation and modulates bone homeostasis in obese mice

Thy1 is a positive regulator of osteoblast differentiation and modulates bone homeostasis in obese mice

Changes of CD90 expression and immunoreactive cell localisation in rat dental pulp after cavity preparation

Two Distinct Processes of Bone-like Tissue Formation by Dental Pulp Cells after Tooth Transplantation

Contact Info

Product

Resources

About