Thylakoid Membrane Fluidity Changes the Response of Isolated Pea Chloroplasts to High Temperature

Zaharieva, Ivelina; Markova, Tz.; Velitchkova, Maya

doi:10.1007/978-94-011-3953-3_426

Cited by 2 publications

(2 citation statements)

References 13 publications

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“…Previous studies have indicated that temperature can induce a change in the double bond index (DBI), with high temperature leading to a decrease in the DBI and low temperature leading to an increase [ 24 , 37 , 38 , 39 ]. Our results were consistent with previous studies: The DBI of total lipids decreased by approximately 10.47% and 10.72% separately under moderate heat stress in wild-type Arabidopsis and hot-1 ( Figure 6 a).…”

Section: Resultsmentioning

confidence: 99%

Quantitative Profiling of Arabidopsis Polar Glycerolipids under Two Types of Heat Stress

Qin

Lin

Jia

et al. 2020

Plants

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At the cellular level, the remodelling of membrane lipids and production of heat shock proteins are the two main strategies whereby plants survive heat stress. Although many studies related to glycerolipids and HSPs under heat stress have been reported separately, detailed alterations of glycerolipids and the role of HSPs in the alterations of glycerolipids still need to be revealed. In this study, we profiled the glycerolipids of wild-type Arabidopsis and its HSP101-deficient mutant hot-1 under two types of heat stress. Our results demonstrated that the alterations of glycerolipids were very similar in wild-type Arabidopsis and hot-1 during heat stress. Although heat acclimation led to a slight decrease of glycerolipids, the decrease of glycerolipids in plants without heat acclimation is more severe under heat shock. The contents of 36:x monogalactosyl diacylglycerol (MGDG) were slightly increased, whereas that of 34:6 MGDG and 34:4 phosphatidylglycerol (PG) were severely decreased during moderate heat stress. Our findings suggested that heat acclimation could reduce the degradation of glycerolipids under heat shock. Synthesis of glycerolipids through the prokaryotic pathway was severely suppressed, whereas that through the eukaryotic pathway was slightly enhanced during moderate heat stress. In addition, HSP101 has a minor effect on the alterations of glycerolipids under heat stress.

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Section: Resultsmentioning

confidence: 99%

Quantitative Profiling of Arabidopsis Polar Glycerolipids under Two Types of Heat Stress

Qin

Lin

Jia

et al. 2020

Plants

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“…We have shown that the controlled perturbation of membrane fluidity achieved by treatment with cholesterol (decreased fluidity) and benzyl alcohol (fluidizing effect) results in an alteration of the energy distribution between both photosystems upon cation-induced stack-unstack transitions (Dobrikova et al, 1997), fluorescent transitions (Busheva et al, 1998) and kinetics of fluorescence decay (Zaharieva et al, 1998a). In vitro alteration of membrane fluidity modifies also the response of thylakoid membranes to short time heat stress (Zaharieva et al, 1998b) or to photoinactivation of PSI and PSII (Velitchkova et al, 2001) and energy distribution between the main pigment-protein complexes upon high light treatment at room and low temperatures (Velitchkova and Popova, 2005).…”

Section: Discussionmentioning

confidence: 99%

Effect of Membrane Fluidity on Photosynthetic Oxygen Production Reactions

Popova

Velitchkova

Zanev

2007

Zeitschrift Für Naturforschung C

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The effect of changes of membrane fluidity on the oxygen evolving capability of isolated thylakoids was investigated. Alteration of the lipid phase fluidity was achieved by incorporation of the plant sterol stigmasterol. Incorporation of stigmasterol in the lipid bilayer of thylakoid membranes results in rigidization of the hydrophobic phase of thylakoid membranes and decreases the degree of packing of the lipid head groups. These changes of lipid order are accompanied by a reduction of oxygen evolution, measured with 1,4-benzoquinone as an electron acceptor, and by a more pronounced inhibition of PSI-mediated electron transport. By analysis of the parameters of oxygen flash yields and oxygen burst under continuous illumination it was shown that after treatment with stigmasterol: 1.) the number of active oxygen-evolving centres decreased; 2.) the remaining active oxygen-evolving centres were not affected in respect to the oscillation pattern; 3.) the contribution of the slow oxygenevolving centres in oxygen burst yield was increased. The effect of stigmasterol was compared with the well-studied effect of cholesterol. Results were discussed in terms of determining the role of lipid order for the organization and functioning of the photosynthetic machinery.

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Thylakoid Membrane Fluidity Changes the Response of Isolated Pea Chloroplasts to High Temperature

Cited by 2 publications

References 13 publications

Quantitative Profiling of Arabidopsis Polar Glycerolipids under Two Types of Heat Stress

Quantitative Profiling of Arabidopsis Polar Glycerolipids under Two Types of Heat Stress

Effect of Membrane Fluidity on Photosynthetic Oxygen Production Reactions

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