Thyme oil and thymol abrogate doxorubicin-induced nephrotoxicity and cardiotoxicity in Wistar rats via repression of oxidative stress and enhancement of antioxidant defense mechanisms

Ahmed, Osama M.; Galaly, Sanaa R.; Raslan, Mai; Mostafa, Mennah-Allah M. A.

doi:10.32604/biocell.2020.08157

Cited by 13 publications

(13 citation statements)

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“…Further, these agents reversed reduced albumin levels. These results are in concurrence with Grespan et al [36]. In addition, thymol treatment rescued hepatocellular damage as evidenced by prevention of any increase in serum ALP, AST, or ALT activities [88].…”

Section: Discussionsupporting

confidence: 89%

“…Thymol, the major Thymus vulgaris component, exhibits hepatoprotective properties [87]. The anti-inflammatory properties of thymus essential oil are at least partially responsible for its hepatoprotective effects [36]. Administration of thyme oil and thymol to DOX-intoxicated rats effectively ameliorated high serum activities of ALT, AST, and ALP and elevated total serum bilirubin level.…”

Section: Discussionmentioning

confidence: 99%

“…Oxidative Medicine and Cellular Longevity DOX-injected control: rats were intraperitoneally administrated DOX at 2 mg/kg b.wt one time/week for 7 weeks. Such group was also administered the equivalent volume of the vehicle (1% CMC kg b.wt) every other day by oral gavage for 7 weeks DOX-injected animals treated with thyme oil: these rats were intraperitoneally administered DOX (2 mg/kg b.wt one time/week) and orally treated with 250 mg thyme oil/ kg b.wt [35,36] every other days by oral gavage for 7 weeks DOX-injected animals treated with thymol: rats within this group were intraperitoneally administered DOX (2 mg/ kg b.wt one time/week) and orally treated with 100 mg thymol/kg b.wt [35,36] every other day for 7 weeks 2.6. Blood and Tissue Sampling.…”

Section: Thymementioning

confidence: 99%

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Thyme Oil and Thymol Counter Doxorubicin-Induced Hepatotoxicity via Modulation of Inflammation, Apoptosis, and Oxidative Stress

Ahmed

Galaly

Mostafa

et al. 2022

Oxidative Medicine and Cellular Longevity

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Doxorubicin (DOX) is an effective anticancer agent with a wide spectrum of activities. However, it has many adverse effects on various organs especially on the liver. Thymol, one of the major components of thyme oil, has biological properties that include anti-inflammatory and antioxidant activities. Thus, this study was designed to examine thyme oil and thymol for their ability to prevent doxorubicin-induced hepatotoxicity in Wistar rats. Hepatotoxicity was induced by an intraperitoneal injection of doxorubicin, at a dose of 2 mg/kg bw/week, for seven weeks. Doxorubicin-injected rats were supplemented with thyme oil and thymol at doses 250 and 100 mg/kg bw, respectively, four times/week by oral gavage for the same period. Treatment of rats with thyme oil and thymol reversed the high serum activities of AST, ALT, and ALP and total bilirubin, AFP, and CA19.9 levels, caused by doxorubicin. Thyme oil and thymol also reduced the high levels of TNF-α and the decreased levels of both albumin and IL-4. These agents ameliorated doxorubicin-induced elevation in hepatic lipid peroxidation and associated reduction in GSH content and GST and GPx activities. Further, the supplementation with thyme oil and thymol significantly augmented mRNA expression of the level of antiapoptotic protein Bcl-2 and significantly downregulated nuclear and cytoplasmic levels of the hepatic apoptotic mediator p53. Thus, thyme oil and thymol successfully counteracted doxorubicin-induced experimental hepatotoxicity via their anti-inflammatory, antioxidant, and antiapoptotic properties.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Thymementioning

confidence: 99%

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Thyme Oil and Thymol Counter Doxorubicin-Induced Hepatotoxicity via Modulation of Inflammation, Apoptosis, and Oxidative Stress

Ahmed

Galaly

Mostafa

et al. 2022

Oxidative Medicine and Cellular Longevity

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“…These results provide clue that TZM-induced LV dysfunction may be related to the induction of apoptosis of cardiomyocytes. The apoptosis of cardiomyocytes is known to be activated by oxidative stress [ 12 , 13 ]. Interestingly, ginsenoside Rg2 has been reported to exhibit antioxidative effects and inhibit the apoptosis of different types of cells [ 14 – 16 ].…”

Section: Discussionmentioning

confidence: 99%

Ginsenoside Rg2 Attenuated Trastuzumab-Induced Cardiotoxicity in Rats

Guang

Zhang

Sun

et al. 2022

BioMed Research International

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Aim. Trastuzumab (TZM) is a monoclonal antibody drug for HER2-positive breast cancer by targeting epidermal growth factor 2, but it has significant cardiotoxicity. Ginsenoside Rg2 has shown a variety of biological activities. This study was aimed at investigating whether Rg2 attenuates TZM-induced cardiotoxicity. Methods. A model of TZM-induced cardiotoxicity was established in Wistar rats, and the rats were pretreated with Rg2. After echocardiography analysis, the rats were killed and the hearts were dissected for RNAseq analysis. Primary human cardiomyocytes (HCMs) were treated with TZM with or without pretreatment with Rg2 and then subjected to a colony formation assay, flow cytometry analysis, and Western blot analysis for the detection of caspase-3, caspase-9, and BAX. Results. TZM induced LV dysfunction in rats, but Rg2 could attenuate TZM-induced LV dysfunction. The mRNA levels of caspase-3, caspase-9, and BAX were significantly higher in TZM-treated rats. The colony formation ability of HCMs was significantly lower in TZM-treated cells but was recovered after pretreatment with Rg2. The apoptosis rate of HCMs was significantly higher in TZM-treated cells but was significantly lower after pretreatment with Rg2. Moreover, protein levels of caspase-3, caspase-9, and BAX were significantly higher in TZM-treated cells but were significantly lower after pretreatment with Rg2. Conclusion. Ginsenoside Rg2 inhibited TZM-induced cardiotoxicity, and the mechanism may be related to the downregulation of the expression of proapoptotic proteins caspase-3, caspase-9, and BAX and the inhibition of TZM-induced apoptosis in cardiomyocytes. Ginsenoside Rg2 has a potential to be applied in patients with breast cancer to prevent TZM-induced cardiotoxicity.

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“…Using nontargeted lipomics based on LC-MS, studied have showed that the characteristics of lipid metabolism disorder in the heart of mice are related to the accumulation of glycerin, phospholipid and ceramide (Ahmed et al, 2020a;Bhat et al, 2019;Jing et al, 2019). The changes of lipid were related to cardiac dysfunction, lipid toxicity, inflammation and insulin resistance (Ahmed et al, 2020a;Ahmed et al, 2020b;Jing et al, 2019;Xu et al, 1999). Glycerin, phospholipid and ceramide might be used as a diagnostic maker of GDM.…”

Section: Discussionmentioning

confidence: 99%

Analysis of specific lipid metabolites in cord blood of patients with gestational diabetes mellitus

Li¹,

Hao²,

CHEN³

et al. 2022

Biocell

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This work aimed to clarify the interaction between the fetus and pregnant patients with gestational diabetes mellitus (GDM), the lipid metabolomics analysis of the fetal umbilical cord blood of GDM patients and normal pregnant women were performed to screen out the specific lipid metabolites for pathogenesis of GDM. From 2019-2020, 21 patients with GDM and 22 normal pregnant women were enrolled in Hexian Memorial Hospital, Panyu District, Guangzhou. The general information such as weight, height, age, body mass index (BMI) before pregnancy were analyzed. Non-targeted metabonomic detection and analysis were performed in umbilical cord plasma using LC-MS method. The age, BMI, delivery methods, and infant weight were different between GDM and control. There were 167 lipid metabolites in umbilical cord blood associated with GDM. Among them, 158 upregulated and 9 downregulated in GDM. There were 13 dysregulated metabolites with C < 30, including Lyso-phosphatidyl-colines

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Thyme oil and thymol abrogate doxorubicin-induced nephrotoxicity and cardiotoxicity in Wistar rats via repression of oxidative stress and enhancement of antioxidant defense mechanisms

Cited by 13 publications

References 0 publications

Thyme Oil and Thymol Counter Doxorubicin-Induced Hepatotoxicity via Modulation of Inflammation, Apoptosis, and Oxidative Stress

Thyme Oil and Thymol Counter Doxorubicin-Induced Hepatotoxicity via Modulation of Inflammation, Apoptosis, and Oxidative Stress

Ginsenoside Rg2 Attenuated Trastuzumab-Induced Cardiotoxicity in Rats

Analysis of specific lipid metabolites in cord blood of patients with gestational diabetes mellitus

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