Thyme oil treatment controls bacterial wilt disease symptoms by inducing antioxidant enzyme activity in Solanum tuberosum

Sallam, Nashwa M. A.; Ali, Esmat F.; Abo-Elyousr, Kamal A. M.; Bereika, Mohamed F. F.; Seleim, Mohamed A. A.

doi:10.1007/s42161-021-00808-2

Cited by 17 publications

(1 citation statement)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[16], and Botrytis cinerea [17]. Its inhibitory effect is mainly attributed to the inhibition of ergosterol biosynthesis by disrupting the cell membrane and cell wall structural integrity of pathogenic fungus, and the inhibitory effect was mainly attributed to the inhibition of membrane lipid peroxidation caused by disrupting pathogenic cell membranes and, cell wall structural integrity [18], inhibiting ergosterol biosynthesis, and promoting cellular reactive oxygen species (ROS) radical accumulation. In addition, Li et al [19] discovered that NOX 2 was involved in maintaining the intracellular reduced nicotinamide adenine dinucleotide phosphate/oxidized nicotinamide adenine dinucleotide phosphate (NADPH/NADP+) balance in Staphylococcus aureus and caused a decrease in intracellular NADPH and ATP and an increase in NADP+ through thymol treatment, thus interfering with mycelial energy metabolism to exert antifungal activity.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory Activity and Mechanism of Action with Thymol against the Blueberry Pathogenic Fungi Caused by Neopestalotiopsis clavispora

Ye,

Shuai,

Luo

et al. 2023

Horticulturae

View full text Add to dashboard Cite

Decay caused by Neopestalotiopsis clavispora is an important postharvest disease of blueberries that seriously affects the commercial value of blueberry fruit. In this paper, we studied the inhibitory activity and mode of action of thymol against the pathogenic fungus of blueberries caused by Neopestalotiopsis clavispora. The results demonstrated that thymol administration could limit mycelial growth in vitro; the inhibitory effect was positively connected with thymol mass concentrations, and the minimal inhibitory concentration (MIC) was 100 mg/L. Further investigations revealed that MIC thymol treatment dramatically reduced the germination of pathogenic spores and led to an increase in the conductivity of the pathogen, leakage of contents, and a decrease in pH. Propidium iodide (PI) staining experiments demonstrated that MIC thymol caused damage to mycelial cell membranes. Additionally, MIC thymol treatment promoted mycelium malondialdehyde content accumulation, inhibited superoxide dismutase (SOD) and catalase (CAT) enzyme activities, decreased adenosine triphosphate (ATP), adenosine diphosphate (ADP), and adenosine monophosphate (AMP) content and energy charge levels, and the fluorescence intensity of mycelium caused by MIC thymol treatment was significantly increased by the 2,7-Dichlorodi-hydrofluorescein diacetate (DCFH-DA) assay. The results of this study indicate that thymol suppresses the proliferation of Neopestalotiopsis clavispora by compromising the integrity of their cell membranes, promoting the accumulation of cellular reactive oxygen species (ROS), and interfering with energy metabolism.

show abstract

Section: Introductionmentioning

confidence: 99%

Inhibitory Activity and Mechanism of Action with Thymol against the Blueberry Pathogenic Fungi Caused by Neopestalotiopsis clavispora

Ye,

Shuai,

Luo

et al. 2023

Horticulturae

View full text Add to dashboard Cite

show abstract

Thymol—a plant‐based monoterpenoid phenol compound of an essential oil for the management of sheath blight disease of rice

Usha,

Pramesh,

Shrikanth

et al. 2024

Pest Management Science

View full text Add to dashboard Cite

Sheath blight of rice is a global disease that significantly reduces rice yield. This study reports the antifungal activity of an active compound of essential oil, thymol, at different concentrations against Rhizoctonia solani (strain RS‐Gvt). In vitro assay results indicated that thymol concentrations (0.5 mg mL−1 and 0.25 mg mL−1) completely inhibited (100%) the mycelial growth of RS‐Gvt (p ≤ 0.01). Microscopic observations of thymol‐treated mycelium of RS‐Gvt at 0.0312 mg mL−1 and above concentrations, revealed a distorted mycelial morphology with deformed hyphae. Hyphae showed a bead‐like appearance, reduction in size, and constriction of the hyphae at uneven points with increased hyphal density often entangling with each other. Further, an on‐field experiment was conducted to study the field bio‐efficacy of thymol for two consecutive Kharif seasons of 2022 and 2023 using a factorial RCBD design. The disease severity was measured as the percent disease index (PDI), and the results of two seasons were pooled. Pathogen (RS‐Gvt) and thymol were inoculated in different combinations/methods as main treatments (M1‐M3), and concentrations of thymol (0.0625–1.0 mg ML−1) as sub‐treatments. The results indicated that all two factors significantly (P = 0.05) influenced the PDI and grain yield. The pooled data of two seasons indicated a significant difference between the main treatments (M1: RS‐Gvt + thymol together; M2: thymol sprayed first followed by RS‐Gvt; M3: RS‐Gvt first followed by thymol spray) on PDI (53.39–59.67) and grain yield (4.16–4.75 t ha−1). M1 exhibited a lower PDI (53.39) and a higher grain yield (4.75 t ha−1) compared to M2 and M3, indicating a protective mode of action of thymol against sheath blight disease of rice. The sub‐treatments have shown significant variation in PDI and grain yield. The PDI and grain yield ranged from 33.70 (at 1 mg mL−1) to 66.21 (at 0.0625 mg mL−1) and 4.18 (at 1 mg mL−1) to 5.26 (at 0.0625 mg mL−1) t ha−1, respectively, among the thymol concentrations. This indicates that increasing concentrations of thymol have negatively influenced the PDI and positively impacted the yield. Therefore, the spray of 1 mg mL−1 of thymol at the potential disease‐infection stage is most effective in controlling the sheath blight disease of rice. This study provides an alternative green bioactive compound for controlling the sheath blight disease, and thymol can be included in developing eco‐friendly integrated disease management practices. © 2024 Society of Chemical Industry.

show abstract

Antibacterial Efficacy of Clove Essential Oil Against Xanthomonas phaseoli pv. phaseoli and Its Influence on Pathogen Responses in Bean

Imran

Abo-Elyousr²,

AL-Harbi³

et al. 2022

Gesunde Pflanzen

View full text Add to dashboard Cite

Thyme oil treatment controls bacterial wilt disease symptoms by inducing antioxidant enzyme activity in Solanum tuberosum

Cited by 17 publications

References 35 publications

Inhibitory Activity and Mechanism of Action with Thymol against the Blueberry Pathogenic Fungi Caused by Neopestalotiopsis clavispora

Inhibitory Activity and Mechanism of Action with Thymol against the Blueberry Pathogenic Fungi Caused by Neopestalotiopsis clavispora

Thymol—a plant‐based monoterpenoid phenol compound of an essential oil for the management of sheath blight disease of rice

Antibacterial Efficacy of Clove Essential Oil Against Xanthomonas phaseoli pv. phaseoli and Its Influence on Pathogen Responses in Bean

Contact Info

Product

Resources

About