Thymectomy in the neonatal rat

Hard, Gordon C.

doi:10.1258/002367775780994664

Cited by 11 publications

(3 citation statements)

References 10 publications

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“…Pre‐operatively, each neonate received subcutaneously a single dose of 0.2 mL of cefazolin solution (50 mg/mL). At 10‐min intervals, the neonate was placed on an ice pack (for 15 min) to induce cryo‐anesthesia (Hard, ). The thorax and neck were wiped with povidone–iodine solution (Behvazan Laboratory, Rasht, Iran).…”

Section: Methodsmentioning

confidence: 99%

“…A small incision was made on the frontal muscle of the trachea and cut open descending continuously along the surface of the thymus. Following TX, the ribs and skin were sutured (Hard, ). The rats were treated with antibiotics (Pen/Strep; Gibco‐BRL) to prevent post‐operational infection, maintained at 37 °C during the recovery phase, and then returned to their respective sterile cages in an environmentally controlled room (24 °C, 12‐L:12‐D photoschedule, 40% relative humidity).…”

Section: Methodsmentioning

confidence: 99%

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Retracted: Is immunosuppression, induced by neonatal thymectomy, compatible with poor reproductive performance in adult male rats?

et al. 2017

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With increasing knowledge that the immune system has a major impact on reproductive ‎health, the potential for cells arising in organs such as the thymus to alleviate oxidative stress ‎has been revealed. This study addresses the impact of neonatal thymectomy on male ‎reproductive function in pubertal and adult animals. Neonatal Sprague Dawley rats were allotted to four treatments consisting of fully thymectomized, partially thymectomized, intact, and sham-operated rats. Half of the rats in each treatment were sacrificed at 40 and the other half at 80 days of age. Testicular volume, ventral prostate and spleen weight, several sperm attributes (concentration, motility, livability, membrane integrity, sperm penetration into mucus, total antioxidant capacity, mitochondrial dehydrogenase activity), plasma superoxide dismutase, glutathione, and testosterone level as well as fertility decreased in thymectomized rats. Adrenal gland weight, sperm malondialdehyde level, indices of oxidative stress, sperm abnormality, testicular and sperm lipid peroxidation, protein carbonylation, and sperm reactive oxygen species generation increased in thymectomized rats. In thymectomized rats, the testes contained high levels of malondialdehyde but low levels of glutathione and ferric-reducing antioxidant power. Epididymal sperm reactive oxygen species, blood lipid peroxidation, and oxidative stress indices ‎‎in blood and spermatozoa were highest in fully thymectomized, intermediate ‎in partially thymectomized, and lowest in both pubertal and mature control rats. Blood levels of superoxide dismutase, lipid peroxidation indices, and ‎testosterone, and mitochondrial adenosine triphosphate and ‎dehydrogenase activities in epididymal spermatozoa were lowest in fully thymectomized, ‎intermediate in partially thymectomized, and highest in both pubertal and mature control rats.‎ The data indicated that increased oxidative stress and mitochondrial dysfunction might play a role in the mechanism of immunosuppression-induced testicular and sperm abnormalities.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Retracted: Is immunosuppression, induced by neonatal thymectomy, compatible with poor reproductive performance in adult male rats?

et al. 2017

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“…Thymectomies in neonatal rodents can be accomplished using a suction technique with reliable outcomes 1 . In adult rats, this technique is associated with an approximate 20% mortality rate and frequently results in an incomplete thymectomy 2 .…”

Section: Introductionmentioning

confidence: 99%

Complete Thymectomy in Adult Rats with Non-invasive Endotracheal Intubation

Rendell

Giamberardino

et al. 2014

JoVE

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Thymectomy in neonatal rodents is an established and reliable procedure for immunological studies. However, in adult rats, complications of hemorrhage and pneumothorax from pleural disruption can result in a significant mortality rate. This protocol is a simple method of rat thymectomy that utilizes a mini-sternotomy and endotracheal intubation. Intubation is accomplished with a non-invasive and easily reproducible method and allows for positive pressure ventilation to prevent pneumothorax and a controlled airway that allows sufficient time for careful thymus dissection to minimize pleural disruption. A 1.5 cm sternal incision decreases contact with mediastinal vessels and pleura, while still providing full visualization of the thymus. Following exposure of the mediastinum, the thymus is removed by blunt dissection under magnification. The pleural space is then sealed by suture closure of the pre-tracheal muscles followed by the application of surgical glue. The thorax is then closed by suture closure of the sternum, followed by suture closure of the skin. All thymectomies were complete as evidenced by immunohistochemical (IHC) staining of mediastinal tissue, and absence of naïve T-cells by flow cytometry, and the procedure had a 96% survival rate. This method is suitable when complete thymectomy with minimal complications is desired for further immunological studies in athymic adult rats.

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Alterations in bone-marrow cellularity following thymectomy in rats

Lipscomb

Scharp

1979

Experientia

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The number of nucleated marrow cells was decreased following neonatal thymectomy in rats, and was corrected by administration of syngeneic lymphoid cells, or by implantation of a syngeneic testis. These results suggest that, in the rat, as has been shown previously in the mouse, lymphoid cells exert parital control over bone marrow cellularity and this effect may be further modulated by sex steroids.

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Thymectomy in the neonatal rat

Cited by 11 publications

References 10 publications

Retracted: Is immunosuppression, induced by neonatal thymectomy, compatible with poor reproductive performance in adult male rats?

Retracted: Is immunosuppression, induced by neonatal thymectomy, compatible with poor reproductive performance in adult male rats?

Complete Thymectomy in Adult Rats with Non-invasive Endotracheal Intubation

Alterations in bone-marrow cellularity following thymectomy in rats

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