Thymic lymphomas in Wistar rats exposed to N‐methyl‐N‐nitrosourea (MNU)

Franchi, Carla Adriene da Silva; Bacchi, Mírian Rumenos Piedade; Padovani, Carlos Roberto; Camargo, João Lauro Viana de

doi:10.1111/j.1349-7006.2003.tb01427.x

Cited by 9 publications

(1 citation statement)

References 21 publications

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“…However, in the case of Long-Evans rats, neoplastic cells affect some lymph nodes, resulting in formation of a tumor mass. On the other hand, malignant lymphomas induced by N-propyl-N-nitrosourea and N-methyl-N-nitrosourea 6 – 8 are known to originate from the thymus. …”

Section: Introductionmentioning

confidence: 99%

Spontaneous Malignant T-cell Lymphoma in a Young Adult Crl:CD (SD) Rat

et al. 2010

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We investigated a case of spontaneous malignant T-cell lymphoma observed in a 19-week-old male Crl:CD (SD) rat. The rat showed paralysis beginning 1 week before euthanasia. Hematological examination revealed marked lymphocytosis without distinct atypia. Macroscopically, hepatosplenomegaly and partial atrophy of the thoracic spinal cord were observed. Microscopically, neoplastic cells infiltrated into the liver, splenic red pulp, bone marrow and epidural space of the thoracic spinal cord, while no neoplastic cells were observed in the thymus and lymph nodes. Moreover, the spinal cord showed focal degeneration due to compression by marked infiltration of neoplastic cells in the subdural space. The neoplastic cells were generally small-sized round cells that had a round nucleus with/without a single nucleolus and scanty cytoplasm. Immunohistochemically, the neoplastic cells were positive for CD3 and CD8 and negative for CD79α. Judging from these results, the present tumor in this young adult rat was diagnosed as malignant T-cell lymphoma.

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Section: Introductionmentioning

confidence: 99%

Spontaneous Malignant T-cell Lymphoma in a Young Adult Crl:CD (SD) Rat

et al. 2010

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High incidence of acute promyelocytic leukemia specifically induced by N-nitroso-N-methylurea (NMU) in Sprague–Dawley rats

et al. 2011

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Carcinogenic agents such as N-methyl-N-nitrosourea can cause tumors. The aims of the present study were to evaluate and classify a subtype of AML (acute myeloid leukemia) that was induced by NMU. According to previous publications, NMU induces not only mammary cancer but also leukemia in Sprague-Dawley (S-D) rats. However, the subtype of leukemia involved in NMU-treated rats is unknown. We found that both organ weight and relative organ weights were significantly higher in NMU-exposed rats than in controls. Morphological changes of rat livers and spleens were assessed by histological evaluation (H&E staining), which found that these tissues were abnormal in appearance. Also, cytological examination of the blood showed immature white blood cells in a smear using Liu's and Papanicolaou stains, indicating that gross abnormalities and histopathological changes were pathologically observed. NMU leukemia incidence was 97.1%. In this study, immunohistochemical (IHC) analysis was valuable in classifying the leukemia of poorly differentiated blasts induced by NMU. Paraffin blocks were stained for MPO, CD3, CD15, CD20, and CD34 markers. The NMU-induced group was positive for MPO, but negative for CD3, CD15, CD20, and CD34. These CD markers suggest that they are useful in helping diagnose APL (M3) leukemia. The model of NMU-induced leukemogenesis in an S-D rat suggests a more definite way to classify APL. This APL will provide an important tool for chemical carcinogenesis and leukemia studies.

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Luteinizing Hormone Receptor Deficiency Increases the Susceptibility to Alkylating Agent-Induced Lymphomagenesis in Mice

Yuan

et al. 2010

HORM CANC

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Previous studies have revealed a close link between luteinizing hormone (LH)/human chorionic gonadotropin (hCG) signaling and oncogenesis in gonadal and nongonadal tissues. To investigate whether genetic ablation of LH receptor (Lhr) affects the animal's oncogenic susceptibility, adult female wild-type (wt), heterozygous, and homozygous Lhr knockout (LhrKO) mice were intraperitoneally injected with an alkylating agent, N-methyl-N-nitrosourea (MNU, 50 mg/kg of body weight). The mice were sacrificed when they were short of breath or 10 months after the injection. The results showed that MNU induced non-Hodgkin's thymic and lymphonodus lymphomas in 70.6% and 100% of heterozygous and homozygous animals, respectively,

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Thymic lymphomas in Wistar rats exposed to N‐methyl‐N‐nitrosourea (MNU)

Cited by 9 publications

References 21 publications

Spontaneous Malignant T-cell Lymphoma in a Young Adult Crl:CD (SD) Rat

Spontaneous Malignant T-cell Lymphoma in a Young Adult Crl:CD (SD) Rat

High incidence of acute promyelocytic leukemia specifically induced by N-nitroso-N-methylurea (NMU) in Sprague–Dawley rats

Luteinizing Hormone Receptor Deficiency Increases the Susceptibility to Alkylating Agent-Induced Lymphomagenesis in Mice

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