Thymic selection of CD8+ single positive cells with a class II major histocompatibility complex-restricted receptor.

Kirberg, Jörg; Baron, Agnès; Jakob, Stephan M.; Rolink, Antonius; Karjalainen, Klaus; Boehmer, Harald von

doi:10.1084/jem.180.1.25

Cited by 391 publications

(361 citation statements)

References 34 publications

Supporting

Mentioning

351

Contrasting

Order By: Relevance

“…BALB/c mice with a wt repertoire of T cells, or TCR-HA T cell receptor transgenic mice with an average frequency of 10% HA-specific CD4 T cells [19], or TCR-HA Rag2 -/-mice, where all § g T cells are CD4 positive and express the transgenic TCR were compared. Rapid tumor growth was observed in all mice (Fig.…”

Section: Tumor Challenge Induces a Th1 Type Cd4 Response Which Howevmentioning

confidence: 99%

“…TCR-HA-transgenic mice expressing a TCR specific for HA 107-119 presented by I-E d [19] and TCR CL4-transgenic mice expressing a TCR specific for HA 512-520 presented by K d [18,27,28] were bred onto BALB/c, or, where indicated, onto a Rag-deficient BALB/c background. BALB/c mice (Taconic, Germantown, NY) of 6-10 weeks of age were used for tumor transplantation experiments.…”

Section: Micementioning

confidence: 99%

“…This tumor was modified to express a fusion of the influenza hemaglutinin (HA) and enhanced green fluorescent protein (EGFP). Transgenic mice expressing TCR specific for either an MHC class-I (TCR-CL4) [18] or class-II (TCR-HA) [19] restricted epitope of HA were employed as source of antigen-specific T cells. Our findings indicate that the rapid growth of this immunogenic tumor creates a situation of "antigen-overload" in which the specific immune response is rapidly "exhausted" unless antigen (tumor) is cleared within a critical time frame.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Visualizing the course of antigen‐specific CD8 and CD4 T cell responses to a growing tumor

et al. 2003

View full text Add to dashboard Cite

Spontaneous tumors frequently express antigens that can be recognized by the immune system but nevertheless manage to evade immune surveillance. To better understand the mechanism of evasion, we followed CD8 and CD4 T cells reacting against a subcutaneously growing tumor, modified to express influenza hemagglutinin (HA) as surrogate tumor antigen. Adoptive transfer of 8,000 antigen-specific CD8 T cells was sufficient to protect against challenge with 1x10 6 tumor cells, while larger numbers of T cells rejected established tumors. HA-specific CD4 T cells could not reject tumors on their own but helped rejection by CD8 T cells. Rejection of the tumor coincided with prolonged survival of expanded antigenspecific CD8 and CD4 T cells, while a failing anti-tumor response was accompanied by transient expansion followed by rapid elimination of antigen-specific T cells. Thus, a highly immunogenic tumor can evade immune surveillance because of an insufficient number of tumor-specific T cells and antigen overload, resulting in exhaustion of the immune response. In this scenario, adoptive immunotherapy rather than vaccination promises successful treatment.

show abstract

Section: Tumor Challenge Induces a Th1 Type Cd4 Response Which Howevmentioning

confidence: 99%

Section: Micementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Visualizing the course of antigen‐specific CD8 and CD4 T cell responses to a growing tumor

et al. 2003

View full text Add to dashboard Cite

show abstract

“…As model T cell epitopes, the following were used: aa residues 91-101 from the mouse 2 315 Ig L chain (33), 323-339 OVA (27), 110 -120 HA (25), and 46 -61 HEL (26). The 2 315 epitope is presented on I-E d class II molecules to CD4 ϩ T cells (6,33), as is the HA epitope (36). The HEL epitope is presented on I-A k class II molecules (20), whereas OVA is presented on I-A d (27).…”

Section: Construction Of Mhc Class Ii-specific Troybodies With T Cellmentioning

confidence: 99%

Efficient Delivery of T Cell Epitopes to APC by Use of MHC Class II-Specific Troybodies

Lunde

Western

Rasmussen

et al. 2002

The Journal of Immunology

View full text Add to dashboard Cite

A major objective in vaccine development is the design of reagents that give strong, specific T cell responses. We have constructed a series of rAb with specificity for MHC class II (I-E). Each has one of four different class II-restricted T cell epitopes genetically introduced into the first C domain of the H chain. These four epitopes are: 91–101 λ2315, which is presented by I-Ed; 110–120 hemagglutinin (I-Ed); 323–339 OVA (I-Ad); and 46–61 hen egg lysozyme (I-Ak). We denote such APC-specific, epitope-containing Ab “Troybodies.” When mixed with APC, all four class II-specific Troybodies were ∼1,000 times more efficient at inducing specific T cell activation in vitro compared with nontargeting peptide Ab. Furthermore, they were 1,000–10,000 times more efficient than synthetic peptide or native protein. Conventional intracellular processing of the Troybodies was required to load the epitopes onto MHC class II. Different types of professional APC, such as purified B cells, dendritic cells, and macrophages, were equally efficient at processing and presenting the Troybodies. In vivo, class II-specific Troybodies were at least 100 times more efficient at targeting APC and activating TCR-transgenic T cells than were the nontargeting peptide Ab. Furthermore, they were 100–100,000 times more efficient than synthetic peptide or native protein. The study shows that class II-specific Troybodies can deliver a variety of T cell epitopes to professional APC for efficient presentation, in vitro as well as in vivo. Thus, Troybodies may be useful as tools in vaccine development.

show abstract

“…Transgenic DO11.10 and HA clonotype 6.5 mice, whose T cells recognize ovalbumin peptide (OVA 323-339 ) and influenza hemagglutinin peptide (HA 111-119 ) respectively in the context of I-A d and I-E d , had been extensively backcrossed to BALB/c [18,19,20]. They were obtained from Andrew Weinberg (Earle A. Chiles Research Institute, OR) and Hyam I. Levitsky, (Johns Hopkins University School of Medicine, Baltimore, MD), and bred in OHSU animal care facilities.…”

Section: Micementioning

confidence: 99%

T cell–antigen-presenting cell interactions visualized in vivo in a model of antigen-specific inflammation

Rosenbaum

Ronick

Song

et al. 2008

Clinical Immunology

View full text Add to dashboard Cite

Videomicroscopy is being used increasingly to characterize the interaction of T cells and antigenpresenting cells (APCs) within lymphatic tissues but has not been reported, to our knowledge, at sites of inflammation. We employed intravital videomicroscopy to study an anterior uveitis model using DO11.10 T cells and ovalbumin (OVA). T cell movement in iris was consistent with a random walk independent of the presence of recognized antigen and had a lateral speed slower than T cells in lymph node. Lingering of T cells adjacent to APCs suggested that they were physically interacting. This apparent contact demonstrated antigen specificity when comparing results from DO11.10 cells with OVA versus bovine serum albumin (BSA) loaded APCs but not when comparing results from OVA-loaded APCs with DO11.10 versus HA clonotype 6.5 T cells. Further studies with this model system should clarify the contribution of T cell-APC communication at a site of inflammation, infection, or immunization.

show abstract

Thymic selection of CD8+ single positive cells with a class II major histocompatibility complex-restricted receptor.

Cited by 391 publications

References 34 publications

Visualizing the course of antigen‐specific CD8 and CD4 T cell responses to a growing tumor

Visualizing the course of antigen‐specific CD8 and CD4 T cell responses to a growing tumor

Efficient Delivery of T Cell Epitopes to APC by Use of MHC Class II-Specific Troybodies

T cell–antigen-presenting cell interactions visualized in vivo in a model of antigen-specific inflammation

Contact Info

Product

Resources

About