Thymic stromal lymphopoietin activation of basophils in patients with allergic asthma is IL-3 dependent

Salter, Brittany; Oliveria, John Paul; Nusca, Graeme; Smith, Steven G; Watson, Rick; Comeau, Micheal; Sehmi, Roma; Gauvreau, Gail M.

doi:10.1016/j.jaci.2015.03.039

Cited by 82 publications

(92 citation statements)

References 26 publications

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“…Our results suggest that the circulating basophils of patients with severe asthma may be overstimulated by higher levels of airway epithelial cytokines, particularly IL-25 and IL-33, when 175 compared to patients with mild/moderate asthma and healthy controls, because the expression of the receptors of these cytokines is positively regulated by the levels of the cytokines themselves. It has been shown that allergen provocation is able to increase the expression of IL-25 and its receptor in the asthmatic bronchial mucosa [16] and also the expression of the TSLP receptor on the basophil membrane [9]. Salter et al [8] investigated the effects of the alarmin cytokines IL-25 and IL-33 on basophils in a model of allergic asthma, and found that they increase basophil activation and migratory potential.…”

Section: Discussionmentioning

confidence: 99%

“…Basophils have an important role in the development of airway inflammation, commonly found in bronchial biopsies of asthmatic patients where their number has been reported to be significantly higher than in healthy subjects [7]. Experimental data show that IL-25, IL-33, and TSLP upregulate the expression of their receptors on the basophil membrane [8,9].…”

Section: Introductionmentioning

confidence: 99%

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Basophil Membrane Expression of Epithelial Cytokine Receptors in Patients with Severe Asthma

Boita

Heffler²,

Omedè³

et al. 2018

Int Arch Allergy Immunol

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Background: Severe asthma is a heterogeneous disease, which is characterized by airway damage and remodeling. All triggers of asthma, such as allergens, bacteria, viruses, and pollutants, interact with the airway epithelial cells, which drive the airway inflammatory response through the release of cytokines, particularly IL-25, IL-33, and thymic stromal lymphopoietin (TSLP). Objectives and Methods: To investigate whether the expression of the IL-25, IL-33, and TSLP receptors on the basophil membrane are associated with asthma severity. Twenty-six patients with asthma (11 severe and 15 moderate/mild) and 10 healthy subjects (controls) were enrolled in the study. The results of the basophil activation test and flow cytometry analysis were assessed to investigate basophil membrane expression of IL-25, TSLP, and IL-33 receptors before and after IgE stimulation. Results: IL-25 and IL-33 receptor expression on the basophil membrane at baseline were significantly higher in patients with severe asthma than in those with mild/moderate asthma or healthy subjects, independent of atopy, eosinophilia, asthma control, and exacerbation frequency. Following IgE stimulation, a significantly higher increase in the IL-25 and IL-33 receptors was observed in mild/moderate versus severe asthma. Conclusions: The high expression of the IL-25 and IL-33 receptors on the basophil membrane of patients with severe asthma indicates an overstimulation of basophils by these cytokines in severe asthma. This finding can possibly be used as a biomarker of asthma severity.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Basophil Membrane Expression of Epithelial Cytokine Receptors in Patients with Severe Asthma

Boita

Heffler²,

Omedè³

et al. 2018

Int Arch Allergy Immunol

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“…Neutralizing antibodies against the TSLP receptor prevent basophils from releasing IL-4 and IL-13 and inhibit CD203c upregulation. Moreover, activation by TSLP depends on an IL-3 receptor component, hence, on the IL-3-specific activation pathway, contributing to IL-3 production of basophils and high surface expression of CCR3 [89]. …”

Section: The Basophil In the Immune Networkmentioning

confidence: 99%

The Evolution of Human Basophil Biology from Neglect towards Understanding of Their Immune Functions

Steiner

Huber

Harrer

et al. 2016

BioMed Research International

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Being discovered long ago basophils have been neglected for more than a century. During the past decade evidence emerged that basophils share features of innate and adaptive immunity. Nowadays, basophils are best known for their striking effector role in the allergic reaction. They hence have been used for establishing new diagnostic tests and therapeutic approaches and for characterizing natural and recombinant allergens as well as hypoallergens, which display lower or diminished IgE-binding activity. However, it was a long way from discovery in 1879 until identification of their function in hypersensitivity reactions, including adverse drug reactions. Starting with a historical background, this review highlights the modern view on basophil biology.

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“…TSLP activates basophils in bone marrow, blood and allergen-challenged lung [93]. Basophils are recruited to the site of allergic inflammation.…”

Section: Progress In Elucidating the Biological Relevance Of Basophilmentioning

confidence: 99%

News in Cellular Allergology: A Review of the Human Mast Cell and Basophil Granulocyte Literature from January 2013 to May 2015

Hoffmann

2015

Int Arch Allergy Immunol

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Mast cell activation releases the mediators associated with type I allergy. As such, the study of mast cell activation is critical for understanding the allergic reaction, and for developing methods to control it. Importantly, another ligand receptor pair (compound 48/80 and MRGPRX2) that activates mast cells in addition to allergen-IgE-FcεRI has been identified. As mast cells mature in tissue from hematopoietic stem cells, their physiology and pathophysiology is difficult to study. Mast cell lines and mast cells cultured from stem cells are often studied instead of tissue mast cells. There has been some progress in the description of the mechanism of the activation of mast cells, substances limiting mast cell activation and in the catalogue of proteases that mast cells express. Basophil granulocytes express FcεRI, bind IgE and respond to allergen crosslinking in a very similar fashion to mast cells. In the recent literature, basophils were mistakenly described as antigen-presenting cells; this has convincingly been disputed in a number of subsequent publications. Their function in physiology and pathophysiology is not known, but they are frequently used to document allergic sensitisation in the basophil activation test. Significant progress has been made in documenting the relevance of basophil activation as a second-line test in allergy diagnosis. Basophil reactivity and sensitivity may reflect symptom severity and allergen threshold, and are used to document and monitor allergy. The physiology and pathophysiology of allergic effector cells remain an important area of research.

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Thymic stromal lymphopoietin activation of basophils in patients with allergic asthma is IL-3 dependent

Cited by 82 publications

References 26 publications

Basophil Membrane Expression of Epithelial Cytokine Receptors in Patients with Severe Asthma

Basophil Membrane Expression of Epithelial Cytokine Receptors in Patients with Severe Asthma

The Evolution of Human Basophil Biology from Neglect towards Understanding of Their Immune Functions

News in Cellular Allergology: A Review of the Human Mast Cell and Basophil Granulocyte Literature from January 2013 to May 2015

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