Thymidine-functionalized silica nanotubes for selective recognition and separation of oligoadenosines

Abstract

Thymidine receptor residues were covalently attached to the inner side wall of silica nanotubes (SNTs). The adsorption capacities of the thymidine-functionalized silica nanotubes (T-SNTs) with nucleic acids and oligonucleic acids were evaluated. The T-SNTs exhibited excellent selectivity both in recognition and separation of adenosine and adenosine-based oligonucleotides, because adenosine and oligoadenosine were selectively bound to the inner surface of T-SNTs by formation of complementary intermolecular hydrogen bonds. On the other hand, guanosine, cytosine and oligoguanosine derivatives were not bound to the T-SNTs. The T-SNTs selectively separated 95% of oligoadenosine from a mixture of equal amounts of oligocytosine and oligoguanosine. Our results imply that the T-SNTs can be used as a selective receptor and separator for the extraction and separation of adenosine and oligoadenosine derivatives in aqueous solution.

Organic–inorganic nanotube hybrids: Organosilica-nanotubes containing ethane, ethylene and acetylene groups

“…So far, several enzymes, such as lysozyme [57], penicillin G acylase [58], or glucose oxidase [59] have been immobilized on silica nanotubes, as well as fluorescent laser dyes [60] or thymidine for adenosines separation [61]. To demonstrate the versatility of the newly synthesized organosilica-nanotubes, the amino acid glycine was grafted on ethylenesilica-nanotubes.…”

Phosphorylated silica nanotubes: preparation and characterization

“…In particular, silica nanotubes (SNTs) arouse special interest because they are biocompatible, photoluminescent, and their surfaces are accessible and can be modified [14]. It is reported that SNTs have extensive applications in organic molecular separation, decomposition of pollutants and inorganic ion sensors, etc [15][16][17][18]. As novel tubular materials, silica nanotubes have good mechanical properties to resist the outer impact and pull forces, possessing the advantages of both functional materials and structural materials.…”

Recent advances in applications of nanomaterials for sample preparation

“…Nano-silica fabricated with AgNPs has been reported as an efficient adsorbent for the dye contaminants in water[63]. Similarly, pure and Ag-doped SiO 2 present reliable property in the adsorption of chlorinated compounds in water[64].Jung et al[65] attached thymidine receptor residues to the inner side wall of silica nanotubes (SNTs) covalently. The composite material T-SNTs exhibited excellent selectivity both in recognition and separation of adenosine and adenosine-based oligonucleotides through the formation of complementary intermolecular hydrogen bonds.…”