Thymidine uptake by chicken kidney cells parasitized by <i>Eimeria tenella</i>: evidence for a diffusable mediating substance

Urquhart, Catriona

doi:10.1017/s0031182000056924

Cited by 4 publications

(2 citation statements)

References 8 publications

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“…The changes in the cells were often accompanied by biochemical modi®ca-tions that included increased proteolytic sensitivity brought about by accumulation of gel-phase lipid in the plasma membrane (Fernando 1983), enriched levels of certain plasma membrane proteins (Fernando et al 1974;Fernando 1983), and altered DNA synthesis (Urquhart 1981b). Conversely, little is known about the eects of developmentally arrested avian Eimeria sporozoites on the morphology or biochemistry of cells, either in the birds or in cell culture.…”

Section: Discussionmentioning

confidence: 99%

Eimeria adenoeides sporozoites: enhanced invasion of cultured cells previously inoculated with Eimeria acervulina sporozoites

Augustine¹

2001

Parasitology Research

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The effects of prior or concurrent administration of Eimeria acervulina on invasion of cultured cells by Eimeria adenoeides sporozoites and possible mechanisms of action were examined. Baby hamster kidney (BHK) cell cultures that were inoculated with E. acervulina sporozoites were significantly more permissive for invasion by E. adenoeides sporozoites than uninoculated cultures. Enhancement of invasion by E. adenoeides did not occur when the two species were inoculated into cultures concurrently, or within 30 h of each other. However, 48 and 72 h after inoculation of BHK cells with E. acervulina, invasion by E. adenoeides sporozoites was significantly greater than invasion in uninoculated cultures. At 96 h postinoculation with E. acervulina, the enhancing effect on invasion was variable. Culture media collected from E. acervulina-inoculated cultures also significantly enhanced invasion by E. adenoeides. Slight changes in proteins of E. acervulina-inoculated versus uninoculated cell cultures were detected by Western blots of biotinylated and nonbiotinylated cells. Biotinylated bands between 10 and 25 kDa increased in the inoculated cultures. In addition, when chicken anti-E. acervulina sporozoite serum was used as a probe, labeling of a 10 kDa antigen increased in the inoculated cultures.

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Section: Discussionmentioning

confidence: 99%

Eimeria adenoeides sporozoites: enhanced invasion of cultured cells previously inoculated with Eimeria acervulina sporozoites

Augustine¹

2001

Parasitology Research

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“…By contrast, in cells that do not have the potential to proliferate, the extra cycles of DNA synthesis do not occur to any significant extent. Urquhart (1981 a) found that in E. tenella-infected chicken kidney cells there is enhanced DNA synthesis in comparison to uninfected cells. She postulated that parasitized cells produce two factors which affect replication.…”

mentioning

confidence: 95%

Host cell response to coccidian infection: an introspective survey

Pasternak

Fernando

1984

Parasitology

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Invasion of chicken caecal and intestinal lamina propria by crypt epithelial cells infected with coccidia

Am²,

Rose³

et al. 1983

Parasitology

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The development of second generation schizonts of Eimeria necatrix and E. tenella was studied with the electron microscope. Invasion of the crypt epithelial cells by merozoites of the first generation schizonts caused changes in the morphology of the infected cells and stimulated their migration into the lamina propria through breaks which appeared in the basement membrane of the crypts. Second generation schizonts developed in the lamina propria within these crypt cells whose epithelial origin was confirmed by their interconnection by desmosomes and tight junctions and by their possession of characteristic microvilli. A comparison is made between this invasion of the lamina propria by parasitized cells and invasion of connective tissue by malignant epithelial cells; the possible mechanisms involved are discussed.

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Thymidine uptake by chicken kidney cells parasitized by Eimeria tenella: evidence for a diffusable mediating substance

Cited by 4 publications

References 8 publications

Eimeria adenoeides sporozoites: enhanced invasion of cultured cells previously inoculated with Eimeria acervulina sporozoites

Eimeria adenoeides sporozoites: enhanced invasion of cultured cells previously inoculated with Eimeria acervulina sporozoites

Host cell response to coccidian infection: an introspective survey

Invasion of chicken caecal and intestinal lamina propria by crypt epithelial cells infected with coccidia

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