Thymol attenuates inflammation in isoproterenol induced myocardial infarcted rats by inhibiting the release of lysosomal enzymes and downregulating the expressions of proinflammatory cytokines

Meeran, Mohamed Fizur Nagoor; Jagadeesh, Govindan Sangaran; Selvaraj, Palanisamy

doi:10.1016/j.ejphar.2015.02.028

Cited by 49 publications

(23 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As one of the most important immune cells, macrophage activation is a hallmark of inflammation and produces ROS, which trigger signal transduction pathways [32]. Although there have been many reports that have extensively described the anti-inflammatory function of thymol, a Chinese herbal medicine [19, 33], a detailed analysis of its molecular targets has not been completed until now. In the present study, an LPS-induced endometritis mouse model was first used to evaluate the anti-inflammatory effects of thymol in vivo , and its complex mechanism was uncovered in LPS-stimulated macrophages in vitro .…”

Section: Discussionmentioning

confidence: 99%

Thymol mitigates lipopolysaccharide-induced endometritis by regulating the TLR4- and ROS-mediated NF-κB signaling pathways

Jiang

Yin

et al. 2017

Oncotarget

View full text Add to dashboard Cite

The purpose of this study was to investigate the effects of thymol on lipopolysaccharide (LPS)-induced inflammatory responses and to clarify the potential mechanism of these effects. LPS-induced mouse endometritis was used to confirm the anti-inflammatory action of thymol in vivo. RAW264.7 cells were used to examine the molecular mechanism and targets of thymol in vitro. In vivo, thymol markedly alleviated LPS-induced pathological injury, myeloperoxidase (MPO) activity, and the production of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in mice. Further studies were performed to examine the expression of the Toll-like receptor 4 (TLR4) -mediated nuclear factor-κB (NF-κB) pathway. These results showed that the expression of the TLR4-mediated NF-κB pathway was inhibited by thymol treatment. In vitro, we observed that thymol dose-dependently inhibited the expression of TNF-α, IL-1β, inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) in LPS-stimulated RAW264.7 cells. Moreover, the results obtained from immunofluorescence assays also indicated that thymol dose-dependently suppressed LPS-induced reactive oxygen species (ROS) production. Silencing of TLR4 inhibited NF-κB pathway activation. Furthermore, H2O2 treatment increased the phosphorylation of p65 and IκBα, which were decreased when treated with N-acetyl cysteine or thymol. In conclusion, the anti-inflammatory effects of thymol are associated with activation of the TLR4 or ROS signaling pathways, contributing to NF-κB activation, thereby alleviating LPS-induced oxidative and inflammatory responses.

show abstract

Section: Discussionmentioning

confidence: 99%

Thymol mitigates lipopolysaccharide-induced endometritis by regulating the TLR4- and ROS-mediated NF-κB signaling pathways

Jiang

Yin

et al. 2017

Oncotarget

View full text Add to dashboard Cite

show abstract

“…For the normal control and CUMS model groups, mice were given an equal volume of 0.3% carboxy methyl cellulose. The levels of thymol administration chosen were similar to those used in previous trials, in which thymol protected against inflammation in isoproterenol induced myocardial infarcted rats [19], ovalbumin induced mouse asthma [21], and cisplatin induced nephrotoxic rats [31]. And these doses were considerably lower than the reported value of the median lethal dose (980 mg/kg in rats) [25].…”

Section: Cums Procedures and Experimental Designmentioning

confidence: 90%

“…Thymol exhibited strong anti-inflammatory activities in the models of osteoarthritis [20], asthma [21], skin inflammation [41], ear edema [42], and peritonitis [43] etc. It has been reported to attenuate inflammation through downregulating the expressions of proinflammatory cytokines, such as TNF-␣ [19,30,44], IL-1␤ [19], and IL-6 [19,44]. Furthermore, thymol could inhibit the activation of NF-B signaling in LPS treated macrophages [45], and down-regulate the MAPK pathway in mammary epithelial cells [46].…”

Section: Effects Of Thymol On Inflammationmentioning

confidence: 97%

“…[17,18]. Thymol has been widely used as an active anti-inflammatory ingredient, which can inhibit the isoproterenol induced inflammation in myocardial infarcted rats [19], attenuate collagenase-induced osteoarthritis [20], and alleviate allergic airway inflammation in ovalbumin-induced mouse asthma [21]. Moreover, studies have reported that thymol has various bioactivities, such as anticancer [22], anti-bacterial [18], and antioxidant [23] properties.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Thymol produces an antidepressant-like effect in a chronic unpredictable mild stress model of depression in mice

Deng

Chen

et al. 2015

Behavioural Brain Research

127

View full text Add to dashboard Cite

“…31,32 However, it has also been reported that ISO treatment downregulated TNF-α production and caused no change in IL-6 production. 33 This suggested that inflammation is a complicated process, and different inflammatory cytokines may have different reactions after β-receptor activation.…”

mentioning

confidence: 99%

PEG-coated gold nanoparticles attenuate β-adrenergic receptor-mediated cardiac hypertrophy

Qiao¹,

Zhu²,

Tian³

et al. 2017

IJN

View full text Add to dashboard Cite

Gold nanoparticles (AuNPs) are widely used as a drug delivery vehicle, which can accumulate in the heart through blood circulation. Therefore, it is very important to understand the effect of AuNPs on the heart, especially under pathological conditions. In this study, we found that PEG-coated AuNPs attenuate β-adrenergic receptor (β-AR)-mediated acute cardiac hypertrophy and inflammation. However, both isoproterenol, a non-selective β-AR agonist, and AuNPs did not induce cardiac function change or cardiac fibrosis. AuNPs exerted an anti-cardiac hypertrophy effect by decreasing β 1 -AR expression and its downstream ERK1/2 hypertrophic pathway. Our results indicated that AuNPs might be safe and have the potential to be used as multi-functional materials (drug carrier systems and anti-cardiac hypertrophy agents).

show abstract

Thymol attenuates inflammation in isoproterenol induced myocardial infarcted rats by inhibiting the release of lysosomal enzymes and downregulating the expressions of proinflammatory cytokines

Cited by 49 publications

References 41 publications

Thymol mitigates lipopolysaccharide-induced endometritis by regulating the TLR4- and ROS-mediated NF-κB signaling pathways

Thymol mitigates lipopolysaccharide-induced endometritis by regulating the TLR4- and ROS-mediated NF-κB signaling pathways

Thymol produces an antidepressant-like effect in a chronic unpredictable mild stress model of depression in mice

PEG-coated gold nanoparticles attenuate β-adrenergic receptor-mediated cardiac hypertrophy

Contact Info

Product

Resources

About

Thymol attenuates inflammation in isoproterenol induced myocardial infarcted rats by inhibiting the release of lysosomal enzymes and downregulating the expressions of proinflammatory cytokines

Cited by 49 publications

References 41 publications

Thymol mitigates lipopolysaccharide-induced endometritis by regulating the TLR4- and ROS-mediated NF-κB signaling pathways

Thymol mitigates lipopolysaccharide-induced endometritis by regulating the TLR4- and ROS-mediated NF-κB signaling pathways

Thymol produces an antidepressant-like effect in a chronic unpredictable mild stress model of depression in mice

PEG-coated gold nanoparticles attenuate &beta;-adrenergic receptor-mediated cardiac hypertrophy

Contact Info

Product

Resources

About

PEG-coated gold nanoparticles attenuate β-adrenergic receptor-mediated cardiac hypertrophy