Thymoquinone, a bioactive component of Nigella sativa Linn seeds or traditional spice, attenuates acute hepatic failure and blocks apoptosis via the MAPK signaling pathway in mice

Yang, Yong; Bai, Ting; Sun, Peng; Lian, Li-Hua; Yao, You-Li; Zheng, Hui-Xing; Li, Xin; Li, Jinbin; Wu, Yan-Ling; Nan, Ji‐Xing

doi:10.1039/c4ra15065a

Cited by 8 publications

(2 citation statements)

References 28 publications

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“…Results of our study are in agreement with earlier studies by Daba and Abdel-Rahman (1998) and Yong et al (2015) suggesting that TQ supplementation could alleviate hepatic toxicity. This protective effect may be due to the ability of TQ to antagonize the enhanced LPO and in turn stabilize the integrity of the cellular membranes leading to preventing or at least decreasing the leakage of liver enzymes.…”

Section: Discussionsupporting

confidence: 93%

Mitigation of Fluoride Toxicity by the Use of Thymoquinone in Adult Male Albino Rat

Anza

Eldin

2015

Ain Shams Journal of Forensic Medicine and Clinical Toxicology

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All rights reserved. cookware (NRC, 2006) and in topical agents (toothpastes, dental varnishes, gels, rinses, tablets, and drops) designed to reduce dental decay. As a result, the consumption of fluoride became uncontrolled and unpredictable often exceeding its therapeutic window, particularly among children (Natalia and Gennadii, 2012). An excessive fluoride consumption has been linked to development of fluorosis, a slow degenerative diseases, affecting teeth and bone tissues (Sarkar et al., 2014), as well as inducing neurological defects (Malin and Christine, 2015). During recent decades, numerous investigations have established the toxicity of fluoride Abstract Background: Fluoride is widely distributed in nature in many forms and its compounds are being used extensively. Increased oxidative stress is proposed to mediate the toxic effects of fluoride on soft tissues. Thymoquinone (TQ), the major bioactive ingredient isolated from Nigella sativa seed, has been studied for its anti-oxidant properties. Accordingly, this work was conducted to investigate the possible protective effects of TQ against sodium fluoride (NaF)-induced hematological and biochemical toxicity in male albino rats Method: Seventy animals were divided into 7 equal groups. Group I served as negative control group. Group II or positive control group received distilled water orally. Group III received TQ orally at a dose of 10 mg/kg for 5 weeks. Group IV and group V were intoxicated with NaF orally in two different doses: 10 mg/kg and 20 mg/kg respectively for 4 weeks. Group VI was given 10 mg/kg TQ one week prior administration of both TQ and Na F at a dose of 10mg/kg for 4 weeks. Similarly group VII received 10 mg/kg TQ for one week then concomitantly with Na F at a dose of 20 mg/kg for 4 weeks. At the end of the experiment, the animals were sacrificed and blood samples were obtained for assessment of hemoglobin (Hb) concentration, red cell count (RBC), total leucocytic count (TLC), platelet count, serum cholesterol, triglycerides (TG) and blood glucose level in addition to liver enzymes, total bilirubin level and total serum proteins. Oxidative indices including antioxidant enzyme superoxide dismutase (SOD), total antioxidant capacity (TAC), thiobarbituric acid reactive substances (TBARS) and advanced oxidation protein product (AOPP) were also assessed. Results: NaF intoxicated groups showed significant alterations of hematological and biochemical indices with significantly depleted SOD, decreased TAC and concomitant increase in TBARS and AOPP. Pretreatment by TQ mitigated both hematological and biochemical changes induced by NaF probably due to its strong antioxidant activity. Conclusions: The results obtained indicate the role of oxidative stress in NaF toxicity and suggest a possible protective effect of TQ against the toxicity of fluoride compounds.

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Section: Discussionsupporting

confidence: 93%

Mitigation of Fluoride Toxicity by the Use of Thymoquinone in Adult Male Albino Rat

Anza

Eldin

2015

Ain Shams Journal of Forensic Medicine and Clinical Toxicology

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“…Nigella sativa , which possesses various biological activities, is often used to treat diseases such as hypertension, asthma, and skin disorders [ 13 ]. Despite being approved for use in food by the United States Food and Drug Administration, it is most commonly used as a spice in food production [ 14 ]. Thymoquinone (TQ,5-isopropyl-2-methyl-1,4-benzoquinone, C 10 H 12 O 2 ; Figure 1 ) is an effective monomer isolated from N. sativa .…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Activity and Action Mechanism of Thymoquinone against Bacillus cereus and Its Spores

Wang

Deng

Wang

et al. 2021

Foods

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In this study, thymoquinone (TQ), a natural active substance, was investigated for its antibacterial activity against Bacillus cereus, and its inhibitory effect on B. cereus in reconstituted infant formula (RIF) was evaluated. In addition, the inhibitory effect of TQ on B. cereus spore germination was explored. The minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of TQ against eight B. cereus strains ranged from 4.0 to 8.0 μg/mL, whereas B. cereus treated with TQ displayed a longer lag phase than the untreated control. TQ exerted a good bactericidal effect on B. cereus in Luria–Bertani broth. In addition, TQ obviously reduced the intracellular ATP concentration of B. cereus, which caused depolarization of the cell membrane, increased the intracellular reactive oxygen species level, impaired the cell morphology, and destroyed proteins or inhibited proteins synthesis. This provides a mechanism for its bacteriostatic effect. TQ also inactivated B. cereus growth in RIF. Moreover, reverse transcription–quantitative polymerase chain reaction illustrated that TQ downregulated the transcription of genes related to hemolysin, non-hemolytic enterotoxin, enterotoxin, and cytotoxin K. Meanwhile, TQ displayed the ability to inhibit the germination of B. cereus spores. These findings indicate that TQ, as an effective natural antimicrobial preservative, has potential applications in controlling food contamination and foodborne diseases caused by B. cereus.

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Health-Promoting Activities of Nigella sativa Fixed Oil

Balbaa

El‐Zeftawy

Abdulmalek

et al. 2020

Black Cumin (Nigella Sativa) Seeds: Chemistry, Technology, Functionality, and Applications

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Thymoquinone, a bioactive component of Nigella sativa Linn seeds or traditional spice, attenuates acute hepatic failure and blocks apoptosis via the MAPK signaling pathway in mice

Cited by 8 publications

References 28 publications

Mitigation of Fluoride Toxicity by the Use of Thymoquinone in Adult Male Albino Rat

Mitigation of Fluoride Toxicity by the Use of Thymoquinone in Adult Male Albino Rat

Antimicrobial Activity and Action Mechanism of Thymoquinone against Bacillus cereus and Its Spores

Health-Promoting Activities of Nigella sativa Fixed Oil

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