Thymoquinone ameliorates chemical induced oxidative stress and β-cell damage in experimental hyperglycemic rats

Sankaranarayanan, Chandrasekaran; Pari, Leelavinothan

doi:10.1016/j.cbi.2011.02.029

Cited by 96 publications

(54 citation statements)

References 32 publications

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“…The ability of TQ to induce glutathione levels has also been confirmed in NO-deficient hypertensive rats, leading to attenuated hypertension and renal damage [53]. Similar effects were reported in the kidneys of Wistar albino rats with induced acute renal toxicity [54], the liver tissue of Wistar albino rats with induced-hepatocarcinoma [55], and the kidney and liver tissues of streptozotocin diabetic rats [56]. Hence, the beneficial effects of TQ against EAE are due to its ability to act as a glutathione inducer, allowing TQ to serve as an anti-oxidant and anti-inflammatory agent.…”

Section: Effects Of Tq On Inflammationmentioning

confidence: 51%

Immunomodulatory and anti-inflammatory action of Nigella sativa and thymoquinone: A comprehensive review

Majdalawieh

Fayyad

2015

International Immunopharmacology

199

105

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Section: Effects Of Tq On Inflammationmentioning

confidence: 51%

Immunomodulatory and anti-inflammatory action of Nigella sativa and thymoquinone: A comprehensive review

Majdalawieh

Fayyad

2015

International Immunopharmacology

199

105

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“…For centuries, particularly in the Middle East, black cumin seeds ( Nigella sativa ) have been used to treat a diverse range of peripheral and central inflammatory related illnesses such as diabetes, asthma, arthritis, colitis, periodontitis and encephalomyelitis (Butt and Sultan, 2010, Padhye et al, 2008, Sankaranarayanan and Pari, 2011, Shabana et al, 2013, Woo et al, 2012). Moreover, unlike some synthetic drugs, the anti-inflammatory properties of black cumin seeds are accompanied by a milieu of health promoting effects on almost every major system (i.e.…”

Section: Introductionmentioning

confidence: 99%

Anti-inflammatory effects of thymoquinone in activated BV-2 microglial cells

Taka

Mazzio

Goodman

et al. 2015

Journal of Neuroimmunology

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Thymoquinone (TQ), the main pharmacological active ingredient within the black cumin seed (Nigella sativa) is believed to be responsible for therapeutic effects on chronic inflammatory conditions such as arthritis, asthma and neurodegeneration. In this study, we evaluated the potential anti-inflammatory role of TQ in lipopolysaccharide (LPS)-stimulated BV-2 murine microglia cells. The results obtained indicated that TQ was effective in reducing NO2- with an IC50 of 5.04 μM, relative to selective iNOS inhibitor LNIL- L-N6-(1-Iminoethyl)lysine (IC50 4.09 μM). TQ mediated reduction in NO2- was found to parallel the decline of iNOS protein expression as confirmed by immunocytochemistry. In the next study, we evaluated the anti-inflammatory effects of TQ on ninety – six (96) cytokines using a RayBio AAM-CYT-3 and 4 cytokine antibody protein array. Data obtained establish a baseline protein expression profile characteristic of resting BV-2 cells in the order of osteopontin > MIP-1alpha > MIP-1g > IGF-1 and MCP-I. In the presence of LPS [1ug/ml], activated BV-2 cells produced a sharp rise in specific pro-inflammatory cytokines/chemokine’s IL-6, IL-12p40/70, CCL12 /MCP-5, CCL2 / MCP-1, and G-CSF which were attenuated by the addition of TQ (10μM). The TQ mediated attenuation of MCP-5, MCP-1 and IL-6 protein in supernatants from activated BV-2 cells were corroborated by independent ELISA and mRNA expression profiling using RT2 Profiler PCR cytokine arrays. Moreover, the data obtained from the RT2 PCR demonstrated a similar pattern where the LPS mediated elevation of mRNA for IL-6, CCL12 /MCP-5, CCL2 / MCP-1 were significantly attenuated by TQ (10μM). Also, in this study, consistent data were obtained for both protein antibody array densitometry and ELISA assays. In addition, TQ was found to reduce LPS mediated elevation in gene expression of Cxcl10 and a number of other cytokines in the panel. These findings demonstrate the significant anti-inflammatory properties of TQ in LPS activated microglial cells. Therefore, the obtained results might indicate the usefulness of TQ in delaying the onset of inflammation-mediated neurodegenerative disorders involving activated microglia cells.

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“…The protective effect of N. sativa against beta cell destruction is attributed to its active constituent, thymoquinone. Treatment with thymoquinone inhibits STZ-induced islet degeneration and necrosis and leads to the partial regeneration of the islet and beta cells of diabetic animals (Abdelmeguid et al, 2010;Kanter, 2009;Sankaranarayanan, Pari, 2011).…”

Section: Nigella Sativamentioning

confidence: 99%

Pancreatic beta cell protection/regeneration with phytotherapy

Hosseini

Shafiee-Nick

Ghorbani

2015

Braz. J. Pharm. Sci.

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Although currently available drugs are useful in controlling early onset complications of diabetes, serious late onset complications appear in a large number of patients. Considering the physiopathology of diabetes, preventing beta cell degeneration and stimulating the endogenous regeneration of islets will be essential approaches for the treatment of insulin-dependent diabetes mellitus. The current review focused on phytochemicals, the antidiabetic effect of which has been proved by pancreatic beta cell protection/regeneration. Among the hundreds of plants that have been investigated for diabetes, a small fraction has shown the regenerative property and was described in this paper. Processes of pancreatic beta cell degeneration and regeneration were described. Also, the proposed mechanisms for the protective/ regenerative effects of such phytochemicals and their potential side effects were discussed. Embora medicamentos disponíveis atualmente sejam úteis no controle de complicações da Diabetes, complicações aparecem em grande número de pacientes. Considerando-se a fisiopatologia do Diabetes, a prevenção da degeneração de células beta e o estímulo da regeneração endógena de ilhotas será abordagem essencial para o tratamento de diabetes mellitus insulino-dependente. A presente revisão aborda compostos fitoquímicos, cujo efeito é provado na proteção/regeneração de células beta de pâncreas. Entre centenas de plantas que têm sido investigadas para o diabetes, pequena fração tem mostrado propriedade regenerativa, que será descrita neste trabalho. Os processos de degeneração e de regeneração das células beta do pâncrease são descritos. Além disso, mecanismos propostos para efeitos de proteção e regeneração desses compostos fitoquímicos e seus possíveis efeitos colaterais também serão discutidos neste trabalho.Unitermos: Diabetes mellitus/tratamento. Diabetes mellitus/fitoterapia. Fitoterapia/Diabetes mellitus. Ilhotas/regeneração endógena. Células beta/proteção. Células beta/regeneração. Pâncreas. Plantas medicinais/propriedades regenerativas.

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Thymoquinone ameliorates chemical induced oxidative stress and β-cell damage in experimental hyperglycemic rats

Cited by 96 publications

References 32 publications

Immunomodulatory and anti-inflammatory action of Nigella sativa and thymoquinone: A comprehensive review

Immunomodulatory and anti-inflammatory action of Nigella sativa and thymoquinone: A comprehensive review

Anti-inflammatory effects of thymoquinone in activated BV-2 microglial cells

Pancreatic beta cell protection/regeneration with phytotherapy

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