Thymoquinone ameliorates some endocrine parameters and histological alteration in a rat model of polycystic ovary syndrome

Javanshir, Sima Taghvaee; Yaghmaei, Parichehreh; Hajebrahimi, Zahra

doi:10.29252/ijrm.16.4.275

Cited by 15 publications

(14 citation statements)

References 34 publications

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“…Therefore, a new treatment strategy with less side effects, more affordable, and with more pleiotropic activity is required. Previous studies have documented the efficacy of several herbal medications such as resveratrol, 15 quercetin, 16 thymoquinone 17 and Ficus deltoidea 18 in restoring function of the ovaries, insulin sensitivity and hormonal profile in experimentally induced PCOS. These studies suggested that the herbal medicines with ovulatory and hypoglycemic effects might be considered as an alternative therapy for PCOS.…”

Section: Introductionmentioning

confidence: 99%

Therapeutic Effects of Silibinin Against Polycystic Ovary Syndrome Induced by Letrozole in Rats via Its Potential Anti-Inflammatory and Anti-Oxidant Activities

Marouf¹,

Ismaeel²,

Hassan³

et al. 2022

JIR

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Background Current therapies for polycystic ovary syndrome (PCOS) are accompanied by unwanted effects. Silibinin; a flavonolignan has pleiotropic activities and favorable safety profile. Purpose To investigate the efficacy of silibinin on estrous cyclicity, inflammation, oxidative stress and ovarian morphology in letrozole-induced PCOS in rats. Methods Forty-eight female Wistar albino rats were divided into 2 sets. Rats of the first set (n = 12), assigned as a negative control (NC) received only the vehicle, rats of the second set (n = 36), assigned as PCOS rats, were given letrozole 1mg/Kg orally for 21 days. On day 21, six rats from the first set and six rats from the second set were euthanized for confirmation of PCOS-induction. The remaining animals from the first set assigned as group 1, those in the second set (n = 30) were equally divided into 5 groups and treated daily for 19 days as follows: group 2 (positive control) received only the vehicle, group 3 treated with metformin 300mg/Kg orally, groups 4 and 5 treated respectively with 100 and 200 mg/Kg silibinin intraperitoneally (IP), and group 6 treated with a combination of metformin 300mg/Kg orally and silibinin 100mg/Kg IP. On day 40, blood samples were examined for luteinizing hormone (LH), testosterone (TS) and estradiol (EST) levels, the anti-inflammatory and antioxidant parameters, ovarian and uterine morphology. Results Silibinin alone or in combination with metformin was found to be effective in restoring the regularity of estrous cycle by ameliorating the abnormal alterations of LH, TS, EST, tumor necrosis factor (TNF)-α, and oxidative status and by resuming the appearance of corpora lutea and decreasing or even total absence of cystic follicles in the ovaries. Conclusion Silibinin was effective in restoring estrous regularities and alleviating hormonal and histomorphological abnormalities of the ovarian and uterine tissues, this could be due to its anti-androgenic, anti-inflammatory and antioxidant properties.

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Section: Introductionmentioning

confidence: 99%

Therapeutic Effects of Silibinin Against Polycystic Ovary Syndrome Induced by Letrozole in Rats via Its Potential Anti-Inflammatory and Anti-Oxidant Activities

Marouf¹,

Ismaeel²,

Hassan³

et al. 2022

JIR

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“…A serious clinical complication that may result in polycystic ovary syndrome or increased estrogen levels is estradiol valerate-induced PCOS (47). Estradiol production is caused by the dominant follicle growing, and high estrogen levels indicate that FSH production will stop through a negative feedback system.…”

Section: Discussionmentioning

confidence: 99%

“…According to research by Taghvaee Javanshir et al, rats can develop PCOS when given estradiol valerate for 25 days. It can cause morphological changes, anovulation, metabolic problems, hormonal changes, and follicular cysts in the ovaries (47). Being overweight is frequently linked to PCOS and is a risk factor for developing other PCOS disorders.…”

Section: Discussionmentioning

confidence: 99%

Ameliorative Effect of Chitosan-Propolis Nanoparticles on the Estradiol Valerate -Induced PCOS in Rat

Hosseini¹,

Khodaei²,

Hasansagha³

et al. 2023

Preprint

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Background: Endocrine conditions that impact women define polycystic ovary syndrome. Previous studies have demonstrated a connection between PCOS and insulin intolerance, oxidative stress, and immune system dysfunction. Additionally, some reports have indicated that propolis has antioxidant properties and that chitosan nanoparticles have beneficial effects on the reproductive system. The goal of the present study is to compare metformin (Met) to chitosan-propolis nanoparticles and explain how they protect against a rat PCOS model caused by estradiol valerate (EV). (as a control treatment). Methods: In order to cause PCOS in rodents, a single subcutaneous injection of estradiol valerate was used, followed by a 42-day oral administration of 500 mg/kg of chitosan-propolis nanoparticles. Four sets of rats were created: the control, PCOS, metformin (PCOS and 150 mg/kg metformin), and chitosan-propolis nanoparticle (PCOS and 500 mg/kg chitosan-propolis nanoparticle) groups. The histopathological examination of the ovaries and measurement of serum ferctors were performed on all of the animals. Estradiol valerate caused PCOS, but chitosan-propolis nanoparticle therapy reversed it. Results: After chitosan-propolis nanoparticle intervention, the body weight and ovarian morphology had improved, and the serum biochemical parameters, including esretogen, progestron, vitamin D, calcium, and the insulin resistance index, had been corrected. These EV-induced changes include decreased SOD activity, elevated MDA levels, and evidence that oxidative stress was increased by EV when chitosan-propolis nanoparticle/Met were administered for 42 straight days. Additionally, certain relative mRNA transcripts, including those for the MCP, IL18, and CRP genes, were markedly upregulated in EV-treated animals. These findings unequivocally demonstrate the beneficial effects of chitosan-propolis nanoparticles/Met in inflammatory disorders. Conclustion: Together, the study's end findings, which are presented here, are consistent with the hypothesis that chitosan-propolis nanoparticle/Met offered protective activity against the harmful effects of EV. Although ameliorative effects may have been implicated, the primary pathway or pathways still need to be clarified.

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“…The number of follicular cysts decreased significantly with thymoquinone treatment along with an increase in the number of primary follicles, Graafian follicles, antral follicles, and corpus luteum. Thymoquinone treatment also reversed the elevated biochemical parameters, including plasma glucose, triglycerides (TGs), total cholesterol (TC), low-density lipoprotein-cholester-ol (LDL-c), high-density lipoprotein-cholesterol (HDL-c), LH, and FSH [35]. Moreover, an experimental study of letrozoleinduced PCOS female Sprague-Dawley rats revealed that N. sativa oil administration (5 and 10 mL/kg) for 7 weeks resulted in a significant increase in the number of rats undergoing regular cycles, the average number of regular cycles, the appearance of corpus luteum, and a decreased number of cystic follicles, along with a reduction of LH, testosterone, FSH, body weight, FBG, TC, LDL-c, TGs, and MDA.…”

Section: Preclinical Studiesmentioning

confidence: 99%

“…Animal study (estradiol valerate-induced PCOS rats) [35] • Significant improvements in ovarian morphology, ovarian function, and ovulation similar to metformin. • Significant reduction of number of follicular cysts along with an increase in number of primary follicles, antral follicles, Graafian follicles and corpus luteum.…”

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confidence: 99%

A Review of Clinical and Preclinical Studies on the Therapeutic Potential of Black Seeds (Nigella sativa) in the Management of Polycystic Ovarian Syndrome (PCOS)

Balasubramanian¹,

Maideen

Muthusamy³

et al. 2023

J Pharmacopuncture

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Objectives: Polycystic ovary syndrome (PCOS) is a condition that occurs frequently among women of reproductive age and is a polygenic, multifactorial, endocrine, and metabolic disorder. PCOS is becoming more common as a result of risk factors such as current lifestyle, overnutrition, and stress. The use of traditional herbal medicine is higher among the global population. Hence, this review article focuses on the potential of Nigella sativa to manage women with PCOS. Methods: A literature search was carried out using databases including Medline, Google Scholar, EBSCO, Embase, and Science Direct, as well as reference lists, to identify relevant publications that support the use of N. sativa in the management of women with PCOS. Results: Several clinical and preclinical studies have demonstrated that the major bioactive constituent of black seed (N. sativa), thymoquinone, has potential for managing women with PCOS. Moreover, N. sativa may help to manage oligomenorrhea and amenorrhea in women with PCOS through its anti-inflammatory and antioxidant properties. Conclusion: N. sativa has potential for use as a herbal medicine for managing women with PCOS as an integrative medicine along with traditional and modern medicine in conjunction with calorie restriction and regular exercise.

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Thymoquinone ameliorates some endocrine parameters and histological alteration in a rat model of polycystic ovary syndrome

Cited by 15 publications

References 34 publications

Therapeutic Effects of Silibinin Against Polycystic Ovary Syndrome Induced by Letrozole in Rats via Its Potential Anti-Inflammatory and Anti-Oxidant Activities

Therapeutic Effects of Silibinin Against Polycystic Ovary Syndrome Induced by Letrozole in Rats via Its Potential Anti-Inflammatory and Anti-Oxidant Activities

Ameliorative Effect of Chitosan-Propolis Nanoparticles on the Estradiol Valerate -Induced PCOS in Rat

A Review of Clinical and Preclinical Studies on the Therapeutic Potential of Black Seeds (Nigella sativa) in the Management of Polycystic Ovarian Syndrome (PCOS)

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