Thymoquinone Attenuates 6-Mercaptopurine Induced Testicular Toxicity in Albino Rats: Possible Mechanisms are Involved

Abdelbaky, Naglaa W.; Abdelazem, Ahmed Z.; Hashem, Khalid S.

doi:10.17582/journal.aavs/2020/8.6.653.660

Cited by 7 publications

(4 citation statements)

References 21 publications

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“…The current AZA-treated group revealed degenerated seminiferous tubules, marked decrease in spermatogenic cells, pyknotic nuclei, and vacuolated cytoplasm. The cytoplasmic vacuolation may be due to Sertoli cell damage since Sertoli cells control the spermatogenic process as explained in a recent research by Abdelbaky et al (2020). The present ultrastructural study of the MTX group revealed several extreme changes in spermatogonia and Sertoli cells.…”

Section: Discussionsupporting

confidence: 64%

RETRACTED ARTICLE: Impact of gervital against histopathological, ultrastructural, and biochemical alterations caused by methotrexate or azathioprine in albino rat testis

Abdul-Hamid

Abdel-Reheim

Hegazy

et al. 2022

Environ Sci Pollut Res

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Methotrexate (MTX) and azathioprine (AZA) are chemotherapeutic, immunosuppressive, cytotoxic drugs with reported adverse effects, including oxidative damage to testis. This study aims to evaluate the potential effect of grape seed extract (GSE; gervital) to prevent testicular damage caused by MTX and AZA. Male albino rats were separated into six groups: group I, normal control group; group II, GSE (150 mg/kg/day); group III, MTX (8 mg/kg/week); group IV, AZA (15 mg/kg/day); group V, GSE (150 mg/kg/day) + MTX (8 mg/kg/week); group VI, GSE (150 mg/kg/day) + AZA (15 mg/kg/day). All rats were sacrificed, blood samples were obtained for testosterone analysis, and testis was removed for histological and ultrastructural studies and oxidation measurements. A reduction in relative body and testis weight, along with a significant decrease in testosterone levels, was observed. Histopathological and ultrastructural alterations induced by MTX or AZA included reduced spermatozoa, sloughing, marked reduction of spermatogenic cells, and pyknosis of some nuclei. Significant oxidative stress manifested as reduced glutathione (GSH) levels and catalase (CAT) and superoxide dismutase (SOD) activities, as well as increased malondialdehyde (MDA) levels. GSE administration showed an ameliorative effect on testosterone levels and histopathological and ultrastructural changes. GSE treatment also suppressed the increases in MDA levels and the decreases in GSH levels and CAT and SOD activities. In conclusion, these findings confirm that GSE is an effective antioxidant that protects testis from histopathological and ultrastructural damage induced by MTX and AZA. Therefore, GSE is a promising candidate for future use to minimize and alleviate MTX and AZA risks. Graphical abstract

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Section: Discussionsupporting

confidence: 64%

RETRACTED ARTICLE: Impact of gervital against histopathological, ultrastructural, and biochemical alterations caused by methotrexate or azathioprine in albino rat testis

Abdul-Hamid

Abdel-Reheim

Hegazy

et al. 2022

Environ Sci Pollut Res

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show abstract

“…The current AZA-treated group revealed degenerated seminiferous tubules, marked decrease in the spermatogenic cells, pyknotic nuclei, and vacuolated cytoplasm. The cytoplasmic vacuolation may be due to Sertoli cell damage, where Sertoli cells control the spermatogenic process as explained by recent research byAbdelbaky et al (2020). The present ultrastructural study of the MTX group revealed several extreme changes in spermatogonia and Sertoli cells Richburg (2000),.…”

supporting

confidence: 64%

Impact of Gervital Against Histopathological, Ultrastructural, and Biochemical Alterations Caused by Methotrexate or Azathioprine in Albino Rats Testis

Abdul-Hamid

Abdel-Reheim

Hegazy

et al. 2022

Preprint

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Methotrexate (MTX) and azathioprine (AZA) are chemotherapeutic, immunosuppressive, cytotoxic drugs with reported adverse impacts such as oxidative damage to testis. This study aims to scrutinize the potential effect of grape seed extract (GSE; gervital) to prevent testicular damage propelled by MTX and AZA. Rats were separated into six groups: Group I, normal control group; Group II, GSE (150 mg/kg/day); Group III, MTX (8 mg/kg/week). Group IV, AZA (15 mg/kg/day). Group V, GSE (150 mg/kg/day) + MTX (8 mg/kg /week); Group VI, GSE (150 mg/kg/day) + AZA (15 mg/kg/day). All rats were sacrificed, blood samples were obtained for testosterone analysis and testis was removed for histological, ultrastructural studies and oxidation measurements. A reduction in body and testes relative weight, along with a significant decrease in testosterone was observed. Histopathological, ultrastructural alterations induced by MTX or AZA include scanty spermatozoa, sloughing, marked reduction of spermatogonia cells, and pyknosis of some nuclei. Significant oxidative stress (OS) manifested by reduced glutathione (GSH) level, catalase (CAT) and superoxide dismutase (SOD) as well as increase in malondialdehyde (MDA) levels. GSE administration showed an ameliorative effect on testosterone, histopathological and ultrastructural changes. GSE treatment also suppressed the increases in MDA levels and the decrease in GSH level, CAT, and SOD activities. Conclusion, our findings confirmed that GSE is an effective antioxidant that prevent and protect testis from the histopathological and ultrastructural damages induced by MTX and AZA. So, GSE is a promising candidate for future use to minimize and alleviate MTX and AZA risks

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“…23 Researchers displayed beneficial effects of TQ to protect cells and tissues from standard chemotherapy-induced damages in in vivo animal models and in vitro human studies. 9,22,[24][25][26][27] Therefore, based on all this evidence, in the current study, the possible protective effect of TQ in two different dosages on BL-induced toxicity on male reproductive parameters was investigated.…”

Section: Introductionmentioning

confidence: 99%

“…23 Researchers displayed beneficial effects of TQ to protect cells and tissues from standard chemotherapy-induced damages in in vivo animal models and in vitro human studies. 9,22,24–27…”

Section: Introductionmentioning

confidence: 99%

Thymoquinone ameliorates bleomycin-induced reproductive toxicity in male Balb/c mice

et al. 2021

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Bleomycin (BL) is a powerful chemotherapy drug that has devastating effects on spermatogenic function and may make cancer survivors at risk of infertility. Protective effects of thymoquinone (TQ), a phytochemical compound with antioxidant and anticancer influences, were investigated on sperm parameters, testicular structures, and sexual hormones in BL-treated mice. Forty-eight adult male Balb/c mice were randomly divided into six groups. Control group received normal saline; BL group received 10 mg/kg BL; TQ7.5 group received 7.5 mg/kg TQ; TQ15 group received 15 mg/kg TQ; BL+TQ7.5 group received 10 mg/kg BL and 7.5 mg/kg TQ; BL + TQ15 group received 10 mg/kg BL and 15 mg/kg TQ. BL was intraperitoneally used every day through 35 days, and TQ was intraperitoneally injected 3 days before administration of BL and continued twice per week for 35 days. Results showed that BL significantly decreased count, viability, morphology, maturity, and progressive movement of sperm, testosterone, seminiferous tubule diameters, the ratio of testis weight to body weight, number of spermatogonia, spermatocytes, spermatids, and Sertoli cells per tubule, and expression of Bcl2l1 and Bcl2l1/Bax ratio, and increased the non-progressive movement and immotile sperm, intermediate and immature sperm, LH, FSH, and malondialdehyde levels, and tunica albuginea thickness compared to the control group ( p < .05). TQ at a level of 7.5 mg/kg ameliorated BL-induced toxicity on measured parameters and returned most of them to the level of the control group. These data suggested TQ in a dose-dependent manner may have positive effects on BL-induced toxicity of the testis in mice model.

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Thymoquinone Attenuates 6-Mercaptopurine Induced Testicular Toxicity in Albino Rats: Possible Mechanisms are Involved

Cited by 7 publications

References 21 publications

RETRACTED ARTICLE: Impact of gervital against histopathological, ultrastructural, and biochemical alterations caused by methotrexate or azathioprine in albino rat testis

RETRACTED ARTICLE: Impact of gervital against histopathological, ultrastructural, and biochemical alterations caused by methotrexate or azathioprine in albino rat testis

Impact of Gervital Against Histopathological, Ultrastructural, and Biochemical Alterations Caused by Methotrexate or Azathioprine in Albino Rats Testis

Thymoquinone ameliorates bleomycin-induced reproductive toxicity in male Balb/c mice

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