Thymus Dependence of Compound 48/80-Induced Mucosal Mast Cell Proliferation

Aldenborg, Frank

doi:10.1159/000234438

Cited by 11 publications

(8 citation statements)

References 20 publications

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“…We have no satisfactory explanation of this phenomenon as yet. The increse in MC number observed in the present experiment may be ascribed to the mucosal mast cell type (MMC) since it is known that in rat peripheral tissues cornpond 48/80 induces proliferation of the MMC [13][14][15]. Although proliferative responses in gastrointestinal mucosa were observed after repeated treatment with compound 48/80, our result may suggest that in brain proliferation may occur 3 h after treatment.…”

Section: Discussionsupporting

confidence: 41%

The intracerebroventricularly administered mast cells degranulator compound 48/80 increases the pituitary-adrenocortical activity in rats

Gàdek-Michalska

Chłap²,

Turoń

et al. 1991

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The effect of brain mast cells degranulation by compound 48/80 on the pituitary-adrenocortical activity, measured indirectly through corticosterone secretion, and the involvement of a histaminergic mechanism in that stimulation was investigated in conscious rats. All the drugs were given intracerebroventricularly (icv), histamine antagonists 15 min prior to compound 48/80. Compound 48/80 induced a significant dose- and time-related increase in the serum corticosterone levels. That increase, measured 1 h after administration of compound 48/80, was moderately diminished by icv pretreatment of rats with mepyramine and cimetidine, histamine H1- and H2-receptor antagonists. Three hours after administration of compound 48/80 mast cells of the thalamus and the hypothalamus were completely degranulated. At the same time the thalamus and the whole brain histamine levels were substantially higher than in the saline-treated control rats. The above results suggest that histamine liberated from the brain mast cells and central histamine receptors play a moderate role in increasing the pituitary-adrenocortical activity by compound 48/80.

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Section: Discussionsupporting

confidence: 41%

The intracerebroventricularly administered mast cells degranulator compound 48/80 increases the pituitary-adrenocortical activity in rats

Gàdek-Michalska

Chłap²,

Turoń

et al. 1991

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“…It thus seems that the subepidermal mast cell represents a second example of a mast cell with a defined anatomical location which exhibits a distinct proteoglycan expression. Like the MMC, the subepidermal mast cell appears to have a low histamine content (Aldenborg & Enerback, 1985) and responds poorly to Compound 48/80 (Aldenborg, 1987). These findings are of interest in relation to recent evidence concerning the derivation and differentiation of the mast cells.…”

Section: Discussionmentioning

confidence: 88%

Histochemical heterogeneity of dermal mast cells in athymic and normal rats

Aldenborg

Enerbäck

1988

Histochem J

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Mucosal mast cells (MMC) and connective tissue mast cells (CTMC) of the rat contain different proteoglycans, which can be distinguished using histochemical methods. The chondroitin sulphate proteoglycan of the MMC, unlike the heparin of the CTMC, does not show fluorescent berberine binding, is susceptible to aldehyde fixatives and stains preferentially with Alcian Blue in a staining sequence with Safranin. The majority of the dermal mast cells are typical CTMC and are located in the deep part of the dermis. Subepidermal mast cells are comparatively few in normal rats but numerous in athymic rats and mice. These cells differ from other dermal mast cells in that they stain preferentially with Alcian Blue and they appear to contain little histamine. We examined some of the histochemical properties of the skin mast cells of female PVG-rnu/rnu rats and their heterozygous littermates aged from 5 to 29 weeks. The thiazine dye-binding of the subepidermal mast cells was partially blocked by formaldehyde fixation and only about half of them showed a weakly fluorescent berberine binding. The critical electrolyte concentration of the Alcian Blue staining of the subepidermal mast cells was between that of CTMC and MMC. Deaminative cleavage with nitrous acid abolished the staining of all skin mast cells, while that of the MMC was unaffected. There were no statistically significant differences in the staining patterns of the dermal mast cells between different ages or groups of rat. These results indicate that the subepidermal mast cells contain a heparin proteoglycan which is, however, different from that of the typical CTMC of other sites. They thus appear to represent a second example of a mast cell within a defined anatomical location exhibiting a distinct proteoglycan expression.

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“…In contrast, there was no evidence in the present study that compound 48/80 induced a mucosal MC hyperplasia, nor was there any significant increase in the RMCP II content in gut tissue. Others [12] failed to find mucosal MC proliferation in neonatal rats [25] or in adult athymic nude rats following compound 48/80 treatment. The reasons for these discrepancies are not clear.…”

Section: Discussionmentioning

confidence: 99%

“…Alternatively, the different results could be related to the use of different strains of ani mals. The response of mucosal MC is known to vary in dif ferent rat strains [12], suggesting that genetic factors could play a role. However, the BN strain used in the present in vestigation was the same as that used where mucosal hy perplasia was stimulated by auto-anti-lgE induction [9,10].…”

Section: Discussionmentioning

confidence: 99%

“…A large fraction o f these ABMC, which were localized almost entirely in the subepidermal region, was found to be resistant to the action of compound 48/80 [12], a potent rat MC secretagogue [13], The current paper investigates the histochcmical and functional properties of ABMC found at non-mucosal sites following chronic treatment with com pound 48/80 and also monitors the recovery of MC function which was affected by compound 48/80 treatment.…”

Section: Introductionmentioning

confidence: 99%

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Mast Cell Recovery following Chronic Treatment with Compound 48/80

Jaffery

Coleman

Huntley

et al. 1994

Int Arch Allergy Immunol

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Histochemical and functional properties of mast cells (MC) in Brown Norway rats recovering from chronic treatment with the MC secretagogue compound 48/ 80 were examined. In the skin, treatment for 5 days with compound 48/80 resulted in a marked decrease in MC subpopulations defined by differential alcian blue/safranin staining. Both safranin-positive connective tissue MC and alcian blue staining MC were reduced in number. This was accompanied by significant decreases in skin histamine and rat MC serine protease I contents and a loss of specific IgE-mediated passive cutaneous anaphylaxis (PCA) activity. The PCA reaction did not return to normal before 2 months after stopping treatment and only when the numbers of safranin-positive connective tissue MC and skin histamine content reached pretreatment levels. The subepidermal alcian blue staining MC not eliminated by the compound 48/80 treatment were formalin resistant (unlike alcian blue staining mucosal MC of the intestine) and apparently played no role in the PCA response. MC numbers, histamine levels, and rat MC serine protease 1 content of the tongue were similarly decreased by compound 48/80. In contrast, mucosal MC of the gut were unaffected by the secretagogue treatment.

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Thymus Dependence of Compound 48/80-Induced Mucosal Mast Cell Proliferation

Cited by 11 publications

References 20 publications

The intracerebroventricularly administered mast cells degranulator compound 48/80 increases the pituitary-adrenocortical activity in rats

The intracerebroventricularly administered mast cells degranulator compound 48/80 increases the pituitary-adrenocortical activity in rats

Histochemical heterogeneity of dermal mast cells in athymic and normal rats

Mast Cell Recovery following Chronic Treatment with Compound 48/80

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